A Specific and Sensitive Immuno-Terahertz Method to Detect Pathogenic Microorganisms
Terahertz (THz)-based sensing technology shows promise for the rapid and efficient detection of unlabeled biomolecules and pathogens, attributed to their significant absorption in the THz range. However, its application has been limited by low sensitivity and specificity. Addressing this, we creativ...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2025-01-01
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| Series: | Advanced Devices & Instrumentation |
| Online Access: | https://spj.science.org/doi/10.34133/adi.0105 |
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| author | Xuehao Wang Ye Tian Fenli Liu Lintao Huang Fulai Zhang Xupeng Fu Yu Li Qingliang Feng Wen Zeng Junli Liang Donghao Cheng Yadong Xu Jingyao Dai Chaojun Song Airong Qian |
| author_facet | Xuehao Wang Ye Tian Fenli Liu Lintao Huang Fulai Zhang Xupeng Fu Yu Li Qingliang Feng Wen Zeng Junli Liang Donghao Cheng Yadong Xu Jingyao Dai Chaojun Song Airong Qian |
| author_sort | Xuehao Wang |
| collection | DOAJ |
| description | Terahertz (THz)-based sensing technology shows promise for the rapid and efficient detection of unlabeled biomolecules and pathogens, attributed to their significant absorption in the THz range. However, its application has been limited by low sensitivity and specificity. Addressing this, we creatively constructed an advanced detection technique named immuno-THz technology, integrating monoclonal antibody (mAb)-based specific molecular recognition with THz sensing. Detection of a variety of pathogenic microorganisms was highly sensitive and specific in our proof-of-concept assay, achieving a limit of detection of 74.57 colony-forming units/ml for Escherichia coli O157:H7, 0.05495 μg/ml for HAdV-B55 Hexon protein, and 0.00479 μg/ml for Staphylococcal enterotoxin B in phosphate-buffered saline solution. The immuno-THz technique substantially enhances detection sensitivity, marking a 100-fold improvement over traditional THz spectroscopy methods, particularly in detecting E. coli O157:H7. The advanced sensitivity and specificity of the immuno-THz technique will offer an improved approach for the detection of pathogenic microorganisms, be applied in a wide range of applications, and impact on food safety and public health and national defense security. |
| format | Article |
| id | doaj-art-b71eb1fbbf274e5496f2fe0bb915ad10 |
| institution | DOAJ |
| issn | 2767-9713 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Advanced Devices & Instrumentation |
| spelling | doaj-art-b71eb1fbbf274e5496f2fe0bb915ad102025-08-20T03:02:21ZengAmerican Association for the Advancement of Science (AAAS)Advanced Devices & Instrumentation2767-97132025-01-01610.34133/adi.0105A Specific and Sensitive Immuno-Terahertz Method to Detect Pathogenic MicroorganismsXuehao Wang0Ye Tian1Fenli Liu2Lintao Huang3Fulai Zhang4Xupeng Fu5Yu Li6Qingliang Feng7Wen Zeng8Junli Liang9Donghao Cheng10Yadong Xu11Jingyao Dai12Chaojun Song13Airong Qian14Lab for Bone Metabolism, Xi’an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences and Biotechnology, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.Lab for Bone Metabolism, Xi’an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences and Biotechnology, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.Interdisciplinary Research Center of Biology & Catalysis, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.Lab for Bone Metabolism, Xi’an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences and Biotechnology, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.Lab for Bone Metabolism, Xi’an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences and Biotechnology, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.Lab for Bone Metabolism, Xi’an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences and Biotechnology, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.Lab for Bone Metabolism, Xi’an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences and Biotechnology, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.Key Laboratory of Special Functional and Smart Polymer Materials of Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.Ministry of Education Key Laboratory of Micro and Nano Systems for Aerospace, School of Mechanical Engineering, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.School of Electronics and Information, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.China Academy of Civil Aviation Science and Technology, Beijing 100028, China.Key Laboratory of Radiation Detection Materials and Devices, Ministry of Industry and Information Technology, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.Air Force Medical University, Air Force Medical Center, PLA, Xi’an, Shaanxi 710072, China.Lab for Bone Metabolism, Xi’an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences and Biotechnology, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.Lab for Bone Metabolism, Xi’an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences and Biotechnology, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, China.Terahertz (THz)-based sensing technology shows promise for the rapid and efficient detection of unlabeled biomolecules and pathogens, attributed to their significant absorption in the THz range. However, its application has been limited by low sensitivity and specificity. Addressing this, we creatively constructed an advanced detection technique named immuno-THz technology, integrating monoclonal antibody (mAb)-based specific molecular recognition with THz sensing. Detection of a variety of pathogenic microorganisms was highly sensitive and specific in our proof-of-concept assay, achieving a limit of detection of 74.57 colony-forming units/ml for Escherichia coli O157:H7, 0.05495 μg/ml for HAdV-B55 Hexon protein, and 0.00479 μg/ml for Staphylococcal enterotoxin B in phosphate-buffered saline solution. The immuno-THz technique substantially enhances detection sensitivity, marking a 100-fold improvement over traditional THz spectroscopy methods, particularly in detecting E. coli O157:H7. The advanced sensitivity and specificity of the immuno-THz technique will offer an improved approach for the detection of pathogenic microorganisms, be applied in a wide range of applications, and impact on food safety and public health and national defense security.https://spj.science.org/doi/10.34133/adi.0105 |
| spellingShingle | Xuehao Wang Ye Tian Fenli Liu Lintao Huang Fulai Zhang Xupeng Fu Yu Li Qingliang Feng Wen Zeng Junli Liang Donghao Cheng Yadong Xu Jingyao Dai Chaojun Song Airong Qian A Specific and Sensitive Immuno-Terahertz Method to Detect Pathogenic Microorganisms Advanced Devices & Instrumentation |
| title | A Specific and Sensitive Immuno-Terahertz Method to Detect Pathogenic Microorganisms |
| title_full | A Specific and Sensitive Immuno-Terahertz Method to Detect Pathogenic Microorganisms |
| title_fullStr | A Specific and Sensitive Immuno-Terahertz Method to Detect Pathogenic Microorganisms |
| title_full_unstemmed | A Specific and Sensitive Immuno-Terahertz Method to Detect Pathogenic Microorganisms |
| title_short | A Specific and Sensitive Immuno-Terahertz Method to Detect Pathogenic Microorganisms |
| title_sort | specific and sensitive immuno terahertz method to detect pathogenic microorganisms |
| url | https://spj.science.org/doi/10.34133/adi.0105 |
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