Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum
[Objective] The aim of this study was to explore the effect of Acer truncatum fallen leaves in Beijing on the accumulation of medicinal active ingredients of Platycodon grandiflorum and to reveal the molecular mechanism of active ingredient synthesis in P . grandiflorum roots through transcriptome...
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| Format: | Article |
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Science Press
2024-12-01
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| Series: | Xibei zhiwu xuebao |
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| Online Access: | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240342?st=article_issue |
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| author | FENG Chaoqun RUAN Kunfei SHI Shaotong LI Sen CHENG Shaoying LIU Zhonghua |
| author_facet | FENG Chaoqun RUAN Kunfei SHI Shaotong LI Sen CHENG Shaoying LIU Zhonghua |
| author_sort | FENG Chaoqun |
| collection | DOAJ |
| description | [Objective] The aim of this study was to explore the effect of Acer truncatum fallen leaves in
Beijing on the accumulation of medicinal active ingredients of Platycodon grandiflorum and to reveal the
molecular mechanism of active ingredient synthesis in P . grandiflorum roots through transcriptome sequencing.
[Methods] We performed transcriptome sequencing using Illumina high throughput sequencing
technology. Gene expression changes in the synthesis pathways of terpenes, flavonoids, and phenylpropanoid
secondary metabolites were analyzed. [Results] A total of 15 727 SSR loci were detected. 753 differentially
expressed genes (DEGs) were annotated into three major categories of the GO classification,
and 314 DEGs were classified into 105 metabolic pathways in the KEGG classification. Compared with the
control group, there were 17, 7, and 26 DEGs involved in the biosynthesis of terpenes, flavonoids, and
phenylpropanoids, respectively. [Conclusion] We found that DEGs in the terpenes and phenylpropanoid
synthesis pathways were mainly up-regulated, while DEGs in the flavonoid synthesis pathway were generally
down-regulated. It is speculated that the synthesis of terpenes and phenylpropanoids is increased,
while the synthesis of flavonoids is decreased. We obtained the transcriptome information of P . grandiflorum
roots and preliminarily predicted the regulatory pathways for the synthesis of terpenes, flavonoids,
and phenylpropanoids in P . grandiflorum . |
| format | Article |
| id | doaj-art-b71e2049f94f42a8bb9773b63ff220c8 |
| institution | Kabale University |
| issn | 1000-4025 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | Science Press |
| record_format | Article |
| series | Xibei zhiwu xuebao |
| spelling | doaj-art-b71e2049f94f42a8bb9773b63ff220c82025-02-10T12:37:52ZzhoScience PressXibei zhiwu xuebao1000-40252024-12-0144121900191410.7606/j.issn.1000-4025.20240342Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatumFENG Chaoqun0RUAN Kunfei1SHI Shaotong2LI Sen3CHENG Shaoying4LIU Zhonghua5College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China; National Engineering Research Center of Forest Breeding and Ecological Restoration, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China; National Engineering Research Center of Forest Breeding and Ecological Restoration, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China; National Engineering Research Center of Forest Breeding and Ecological Restoration, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China; National Engineering Research Center of Forest Breeding and Ecological Restoration, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China; National Engineering Research Center of Forest Breeding and Ecological Restoration, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China; National Engineering Research Center of Forest Breeding and Ecological Restoration, Beijing 100083, China[Objective] The aim of this study was to explore the effect of Acer truncatum fallen leaves in Beijing on the accumulation of medicinal active ingredients of Platycodon grandiflorum and to reveal the molecular mechanism of active ingredient synthesis in P . grandiflorum roots through transcriptome sequencing. [Methods] We performed transcriptome sequencing using Illumina high throughput sequencing technology. Gene expression changes in the synthesis pathways of terpenes, flavonoids, and phenylpropanoid secondary metabolites were analyzed. [Results] A total of 15 727 SSR loci were detected. 753 differentially expressed genes (DEGs) were annotated into three major categories of the GO classification, and 314 DEGs were classified into 105 metabolic pathways in the KEGG classification. Compared with the control group, there were 17, 7, and 26 DEGs involved in the biosynthesis of terpenes, flavonoids, and phenylpropanoids, respectively. [Conclusion] We found that DEGs in the terpenes and phenylpropanoid synthesis pathways were mainly up-regulated, while DEGs in the flavonoid synthesis pathway were generally down-regulated. It is speculated that the synthesis of terpenes and phenylpropanoids is increased, while the synthesis of flavonoids is decreased. We obtained the transcriptome information of P . grandiflorum roots and preliminarily predicted the regulatory pathways for the synthesis of terpenes, flavonoids, and phenylpropanoids in P . grandiflorum .http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240342?st=article_issueplatycodon grandiflorumacer truncatumtranscriptomedifferentially expressed genes (deg)secondary metabolitessynthetic regulation |
| spellingShingle | FENG Chaoqun RUAN Kunfei SHI Shaotong LI Sen CHENG Shaoying LIU Zhonghua Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum Xibei zhiwu xuebao platycodon grandiflorum acer truncatum transcriptome differentially expressed genes (deg) secondary metabolites synthetic regulation |
| title | Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum |
| title_full | Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum |
| title_fullStr | Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum |
| title_full_unstemmed | Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum |
| title_short | Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum |
| title_sort | transcriptomic analysis of the root system of platycodon grandiflorum in response to the fallen leaves of acer truncatum |
| topic | platycodon grandiflorum acer truncatum transcriptome differentially expressed genes (deg) secondary metabolites synthetic regulation |
| url | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240342?st=article_issue |
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