Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum
[Objective] The aim of this study was to explore the effect of Acer truncatum fallen leaves in Beijing on the accumulation of medicinal active ingredients of Platycodon grandiflorum and to reveal the molecular mechanism of active ingredient synthesis in P . grandiflorum roots through transcriptome...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Science Press
2024-12-01
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| Series: | Xibei zhiwu xuebao |
| Subjects: | |
| Online Access: | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240342?st=article_issue |
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| Summary: | [Objective] The aim of this study was to explore the effect of Acer truncatum fallen leaves in
Beijing on the accumulation of medicinal active ingredients of Platycodon grandiflorum and to reveal the
molecular mechanism of active ingredient synthesis in P . grandiflorum roots through transcriptome sequencing.
[Methods] We performed transcriptome sequencing using Illumina high throughput sequencing
technology. Gene expression changes in the synthesis pathways of terpenes, flavonoids, and phenylpropanoid
secondary metabolites were analyzed. [Results] A total of 15 727 SSR loci were detected. 753 differentially
expressed genes (DEGs) were annotated into three major categories of the GO classification,
and 314 DEGs were classified into 105 metabolic pathways in the KEGG classification. Compared with the
control group, there were 17, 7, and 26 DEGs involved in the biosynthesis of terpenes, flavonoids, and
phenylpropanoids, respectively. [Conclusion] We found that DEGs in the terpenes and phenylpropanoid
synthesis pathways were mainly up-regulated, while DEGs in the flavonoid synthesis pathway were generally
down-regulated. It is speculated that the synthesis of terpenes and phenylpropanoids is increased,
while the synthesis of flavonoids is decreased. We obtained the transcriptome information of P . grandiflorum
roots and preliminarily predicted the regulatory pathways for the synthesis of terpenes, flavonoids,
and phenylpropanoids in P . grandiflorum . |
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| ISSN: | 1000-4025 |