Profile of molecular markers of Sulfadoxine-Pyrimethamine-resistant Plasmodium falciparum in individuals living in southern area of Brazzaville, Republic of Congo
Background: Although the seasonal and perennial malaria chemopreventions are not implemented in the Republic of Congo, resistance to Sulfadoxine-pyrimethamine (SP) threatens the intermittent preventive treatment during pregnancy (IPTp-SP) and others treatments using the drug. The objective of this s...
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Elsevier
2024-12-01
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| Series: | International Journal for Parasitology: Drugs and Drug Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211320724000502 |
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| author | Jean Claude Djontu Marcel Tapsou Baina Jacque Dollon Mbama Ntabi Abel Lissom Dieu Merci Umuhoza Naura veil Assioro Doulamo Christevy Jeanney Vouvoungui Reauchelvy Kamal Boumpoutou Alain Maxime Mouanga Etienne Nguimbi Francine Ntoumi |
| author_facet | Jean Claude Djontu Marcel Tapsou Baina Jacque Dollon Mbama Ntabi Abel Lissom Dieu Merci Umuhoza Naura veil Assioro Doulamo Christevy Jeanney Vouvoungui Reauchelvy Kamal Boumpoutou Alain Maxime Mouanga Etienne Nguimbi Francine Ntoumi |
| author_sort | Jean Claude Djontu |
| collection | DOAJ |
| description | Background: Although the seasonal and perennial malaria chemopreventions are not implemented in the Republic of Congo, resistance to Sulfadoxine-pyrimethamine (SP) threatens the intermittent preventive treatment during pregnancy (IPTp-SP) and others treatments using the drug. The objective of this study was to determine the prevalence of molecular markers of P.falciparum resistance to SP in individuals with microscopic malaria infection in the south of Brazzaville. Methods: Two parallel surveys (health facilities and community-based cross sectional studies) were carried out in urban and rural areas in southern Brazzaville. Between March and October 2021, blood samples were collected from 328 P. falciparum microscopic positive individuals (1–83 years old, and sex ratio female/male of 1.1) to characterize dhfr and dhps genes involved in the P.falciparum resistance to SP. Restriction Fragment Length Polymorphism PCR was used for the detection of mutations within these parasite genes. Results: High prevalence of mutations was reported within Pfdhfr gene: N51I; 328/328 (100%) ratio (prevalence) [95 CI uncertainty], C59R; 317/328 (96.6 %) [94.1–98.1%], S108N; 326/326 (100%), N164L; 3/326 (0.9%) [0.3–2.7%], and Pfdhps gene: A437G; 292/327 (89.3%) [85.5–92.2%], K540E; 140/327(42.8 %) [37.6–48.2%], A581G; 136/325 (41.8%) [36.6–42.3%]. The quintuple mutant (N51I + C59R + S108N + A437G + K540E) and sextuple mutant haplotypes (N51I + C59R + S108N + A437G + K540E + A581G) were reported for 11/144 (7.6%) [4.3–13.2%] and 5/144 (3.4%) [1.5–7.9%]) of the participants respectively. The K540E and A437G mutants were more prevalent in the rural community; 81/139 (58.3%) [50.0–66.1%] and 135/139 (97.1%) [92.8–98.9%] respectively) than in the urban community; 21/50 (46.3%) [33.7–59.4%] and 47/54(87.0%) [75.6–93.6%] (p = 0.004 and p˂0.0001 respectively) Conclusion: These results indicate high prevalence of SP resistance mutations within the dhfr and dhps genes of P. falciparum isolates circulating in study sites, which may limit the efficacy of treatments using SP in these settings. |
| format | Article |
| id | doaj-art-b715de4d52484037aae54e1c4f05db37 |
| institution | DOAJ |
| issn | 2211-3207 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Drugs and Drug Resistance |
| spelling | doaj-art-b715de4d52484037aae54e1c4f05db372025-08-20T02:50:26ZengElsevierInternational Journal for Parasitology: Drugs and Drug Resistance2211-32072024-12-012610056910.1016/j.ijpddr.2024.100569Profile of molecular markers of Sulfadoxine-Pyrimethamine-resistant Plasmodium falciparum in individuals living in southern area of Brazzaville, Republic of CongoJean Claude Djontu0Marcel Tapsou Baina1Jacque Dollon Mbama Ntabi2Abel Lissom3Dieu Merci Umuhoza4Naura veil Assioro Doulamo5Christevy Jeanney Vouvoungui6Reauchelvy Kamal Boumpoutou7Alain Maxime Mouanga8Etienne Nguimbi9Francine Ntoumi10Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Corresponding author. Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo.Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, CongoFondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, CongoFondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Department of Zoology, Faculty of Science, University of Bamenda, Bamenda, CameroonFondation Congolaise pour la Recherche Médicale, Brazzaville, CongoFondation Congolaise pour la Recherche Médicale, Brazzaville, CongoFondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, CongoFondation Congolaise pour la Recherche Médicale, Brazzaville, CongoFondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Faculté des Sciences de la Santé, Université Marien Ngouabi, Brazzaville, CongoFaculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, CongoFondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Institute for Tropical Medicine, University of Tübingen, Tübingen, Germany; Corresponding author. Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo.Background: Although the seasonal and perennial malaria chemopreventions are not implemented in the Republic of Congo, resistance to Sulfadoxine-pyrimethamine (SP) threatens the intermittent preventive treatment during pregnancy (IPTp-SP) and others treatments using the drug. The objective of this study was to determine the prevalence of molecular markers of P.falciparum resistance to SP in individuals with microscopic malaria infection in the south of Brazzaville. Methods: Two parallel surveys (health facilities and community-based cross sectional studies) were carried out in urban and rural areas in southern Brazzaville. Between March and October 2021, blood samples were collected from 328 P. falciparum microscopic positive individuals (1–83 years old, and sex ratio female/male of 1.1) to characterize dhfr and dhps genes involved in the P.falciparum resistance to SP. Restriction Fragment Length Polymorphism PCR was used for the detection of mutations within these parasite genes. Results: High prevalence of mutations was reported within Pfdhfr gene: N51I; 328/328 (100%) ratio (prevalence) [95 CI uncertainty], C59R; 317/328 (96.6 %) [94.1–98.1%], S108N; 326/326 (100%), N164L; 3/326 (0.9%) [0.3–2.7%], and Pfdhps gene: A437G; 292/327 (89.3%) [85.5–92.2%], K540E; 140/327(42.8 %) [37.6–48.2%], A581G; 136/325 (41.8%) [36.6–42.3%]. The quintuple mutant (N51I + C59R + S108N + A437G + K540E) and sextuple mutant haplotypes (N51I + C59R + S108N + A437G + K540E + A581G) were reported for 11/144 (7.6%) [4.3–13.2%] and 5/144 (3.4%) [1.5–7.9%]) of the participants respectively. The K540E and A437G mutants were more prevalent in the rural community; 81/139 (58.3%) [50.0–66.1%] and 135/139 (97.1%) [92.8–98.9%] respectively) than in the urban community; 21/50 (46.3%) [33.7–59.4%] and 47/54(87.0%) [75.6–93.6%] (p = 0.004 and p˂0.0001 respectively) Conclusion: These results indicate high prevalence of SP resistance mutations within the dhfr and dhps genes of P. falciparum isolates circulating in study sites, which may limit the efficacy of treatments using SP in these settings.http://www.sciencedirect.com/science/article/pii/S2211320724000502Plasmodium falciparumSulfadoxine-pyrimethamineMutationRepublic of Congo |
| spellingShingle | Jean Claude Djontu Marcel Tapsou Baina Jacque Dollon Mbama Ntabi Abel Lissom Dieu Merci Umuhoza Naura veil Assioro Doulamo Christevy Jeanney Vouvoungui Reauchelvy Kamal Boumpoutou Alain Maxime Mouanga Etienne Nguimbi Francine Ntoumi Profile of molecular markers of Sulfadoxine-Pyrimethamine-resistant Plasmodium falciparum in individuals living in southern area of Brazzaville, Republic of Congo International Journal for Parasitology: Drugs and Drug Resistance Plasmodium falciparum Sulfadoxine-pyrimethamine Mutation Republic of Congo |
| title | Profile of molecular markers of Sulfadoxine-Pyrimethamine-resistant Plasmodium falciparum in individuals living in southern area of Brazzaville, Republic of Congo |
| title_full | Profile of molecular markers of Sulfadoxine-Pyrimethamine-resistant Plasmodium falciparum in individuals living in southern area of Brazzaville, Republic of Congo |
| title_fullStr | Profile of molecular markers of Sulfadoxine-Pyrimethamine-resistant Plasmodium falciparum in individuals living in southern area of Brazzaville, Republic of Congo |
| title_full_unstemmed | Profile of molecular markers of Sulfadoxine-Pyrimethamine-resistant Plasmodium falciparum in individuals living in southern area of Brazzaville, Republic of Congo |
| title_short | Profile of molecular markers of Sulfadoxine-Pyrimethamine-resistant Plasmodium falciparum in individuals living in southern area of Brazzaville, Republic of Congo |
| title_sort | profile of molecular markers of sulfadoxine pyrimethamine resistant plasmodium falciparum in individuals living in southern area of brazzaville republic of congo |
| topic | Plasmodium falciparum Sulfadoxine-pyrimethamine Mutation Republic of Congo |
| url | http://www.sciencedirect.com/science/article/pii/S2211320724000502 |
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