Destabilized green fluorescent protein detects rapid removal of transcription blocks after genotoxic exposure

High stabilities of reporter proteins and their messenger RNAs (mRNAs) interfere with the detection of rapid transient changes in gene expression, such as transcriptional blocks posed by genotoxic DNA lesions. We have modified a green fluorescent protein (GFP) gene within the episomal pMARS vector b...

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Bibliographic Details
Main Authors: Nataliya Kitsera, Andriy Khobta, Bernd Epe
Format: Article
Language:English
Published: Taylor & Francis Group 2007-08-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/000112479
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