Vaccinomics-driven selection and validation of protective salivary antigens from the argasid tick Ornithodoros moubata
Ornithodoros moubata serves as primary vector of African swine fever and tick-borne human relapsing fever in Africa. Developing an effective vaccine targeting this argasid tick would significantly enhance disease control measures. To identify potential vaccine targets, the recently characterised sia...
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| Format: | Article |
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Elsevier
2025-05-01
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| Series: | Ticks and Tick-Borne Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1877959X25000470 |
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| author | Ana Laura Cano-Argüelles Ana Oleaga María González-Sánchez Rocío Vizcaíno-Marín Ricardo Pérez-Sánchez |
| author_facet | Ana Laura Cano-Argüelles Ana Oleaga María González-Sánchez Rocío Vizcaíno-Marín Ricardo Pérez-Sánchez |
| author_sort | Ana Laura Cano-Argüelles |
| collection | DOAJ |
| description | Ornithodoros moubata serves as primary vector of African swine fever and tick-borne human relapsing fever in Africa. Developing an effective vaccine targeting this argasid tick would significantly enhance disease control measures. To identify potential vaccine targets, the recently characterised sialome of O. moubata was analysed using a vaccinomics approach. This led to the identification of a set of salivary secreted proteins predicted to be antigenic and implicated in the regulation of blood-feeding and host immune defences. The objective of this study was to evaluate the protective potential of seven of these proteins, namely Complement inhibitor (OmCI), Cyclophilin (OmCPH), Hypothetical protein 275 (OmH275), Peroxiredoxin (OmPXR), Calreticulin (OmCLR), Neprilysin (OmNEP), and Superoxide dismutase (OmSOD). These candidates were produced as recombinant proteins, formulated with Montanide adjuvant, and administered individually to different groups of rabbits. Adult and nymphal-3 specimens of O. moubata and Ornithodoros erraticus (the Mediterranean vector of ASF and TBRF) were allowed to feed on the vaccinated rabbits, and the ticks’ feeding performance, survival, and reproduction rates were assessed. OmH275, OmPXR, OmCPH, and OmCLR conferred 20 %–32 % protection against O. moubata and/or O. erraticus, whereas OmCI, OmNEP, and OmSOD afforded 2 %–17 % protection against one or both tick species. Consequently, OmH275, OmPXR, OmCPH, and OmCLR were deemed suitable candidates for inclusion in the development of anti-Ornithodoros cocktail vaccines, while OmCI, OmNEP, and OmSOD were considered less promising for tick vaccine development. These findings validate the vaccinomics pipeline, identifying four of seven candidates (57 %) as viable antigens for Ornithodoros tick vaccines. |
| format | Article |
| id | doaj-art-b6fee4e3f3b04190aec6161d56018df0 |
| institution | DOAJ |
| issn | 1877-9603 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ticks and Tick-Borne Diseases |
| spelling | doaj-art-b6fee4e3f3b04190aec6161d56018df02025-08-20T03:19:56ZengElsevierTicks and Tick-Borne Diseases1877-96032025-05-0116310248310.1016/j.ttbdis.2025.102483Vaccinomics-driven selection and validation of protective salivary antigens from the argasid tick Ornithodoros moubataAna Laura Cano-Argüelles0Ana Oleaga1María González-Sánchez2Rocío Vizcaíno-Marín3Ricardo Pérez-Sánchez4Parasitología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, SpainParasitología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, SpainParasitología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, SpainParasitología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, SpainCorresponding author.; Parasitología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA, CSIC), Cordel de Merinas, 40-52, 37008 Salamanca, SpainOrnithodoros moubata serves as primary vector of African swine fever and tick-borne human relapsing fever in Africa. Developing an effective vaccine targeting this argasid tick would significantly enhance disease control measures. To identify potential vaccine targets, the recently characterised sialome of O. moubata was analysed using a vaccinomics approach. This led to the identification of a set of salivary secreted proteins predicted to be antigenic and implicated in the regulation of blood-feeding and host immune defences. The objective of this study was to evaluate the protective potential of seven of these proteins, namely Complement inhibitor (OmCI), Cyclophilin (OmCPH), Hypothetical protein 275 (OmH275), Peroxiredoxin (OmPXR), Calreticulin (OmCLR), Neprilysin (OmNEP), and Superoxide dismutase (OmSOD). These candidates were produced as recombinant proteins, formulated with Montanide adjuvant, and administered individually to different groups of rabbits. Adult and nymphal-3 specimens of O. moubata and Ornithodoros erraticus (the Mediterranean vector of ASF and TBRF) were allowed to feed on the vaccinated rabbits, and the ticks’ feeding performance, survival, and reproduction rates were assessed. OmH275, OmPXR, OmCPH, and OmCLR conferred 20 %–32 % protection against O. moubata and/or O. erraticus, whereas OmCI, OmNEP, and OmSOD afforded 2 %–17 % protection against one or both tick species. Consequently, OmH275, OmPXR, OmCPH, and OmCLR were deemed suitable candidates for inclusion in the development of anti-Ornithodoros cocktail vaccines, while OmCI, OmNEP, and OmSOD were considered less promising for tick vaccine development. These findings validate the vaccinomics pipeline, identifying four of seven candidates (57 %) as viable antigens for Ornithodoros tick vaccines.http://www.sciencedirect.com/science/article/pii/S1877959X25000470Ornithodoros moubata vaccineProtective salivary antigensCyclophilinHypothetical protein 275PeroxiredoxinCalreticulin |
| spellingShingle | Ana Laura Cano-Argüelles Ana Oleaga María González-Sánchez Rocío Vizcaíno-Marín Ricardo Pérez-Sánchez Vaccinomics-driven selection and validation of protective salivary antigens from the argasid tick Ornithodoros moubata Ticks and Tick-Borne Diseases Ornithodoros moubata vaccine Protective salivary antigens Cyclophilin Hypothetical protein 275 Peroxiredoxin Calreticulin |
| title | Vaccinomics-driven selection and validation of protective salivary antigens from the argasid tick Ornithodoros moubata |
| title_full | Vaccinomics-driven selection and validation of protective salivary antigens from the argasid tick Ornithodoros moubata |
| title_fullStr | Vaccinomics-driven selection and validation of protective salivary antigens from the argasid tick Ornithodoros moubata |
| title_full_unstemmed | Vaccinomics-driven selection and validation of protective salivary antigens from the argasid tick Ornithodoros moubata |
| title_short | Vaccinomics-driven selection and validation of protective salivary antigens from the argasid tick Ornithodoros moubata |
| title_sort | vaccinomics driven selection and validation of protective salivary antigens from the argasid tick ornithodoros moubata |
| topic | Ornithodoros moubata vaccine Protective salivary antigens Cyclophilin Hypothetical protein 275 Peroxiredoxin Calreticulin |
| url | http://www.sciencedirect.com/science/article/pii/S1877959X25000470 |
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