Speciation of Potentially Carcinogenic Trace Nickel(II) Ion Levels in Human Saliva: A Sequential Metabolomics-Facilitated High-Field <sup>1</sup>H NMR Investigation
<b>Introduction/Objectives:</b> Since the biological activities and toxicities of ‘foreign’ and/or excess levels of metal ions are predominantly determined by their precise molecular nature, here we have employed high-resolution <sup>1</sup>H NMR analysis to explore the ‘spec...
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| author | Kayleigh Hunwin Georgina Page Mark Edgar Mohammed Bhogadia Martin Grootveld |
| author_facet | Kayleigh Hunwin Georgina Page Mark Edgar Mohammed Bhogadia Martin Grootveld |
| author_sort | Kayleigh Hunwin |
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| description | <b>Introduction/Objectives:</b> Since the biological activities and toxicities of ‘foreign’ and/or excess levels of metal ions are predominantly determined by their precise molecular nature, here we have employed high-resolution <sup>1</sup>H NMR analysis to explore the ‘speciation’ of paramagnetic Ni(II) ions in human saliva, a potentially rich source of biomolecular Ni(II)-complexants/chelators. These studies are of relevance to the <i>in vivo</i> corrosion of nickel-containing metal alloy dental prostheses (NiC-MADPs) in addition to the dietary or adverse toxicological intake of Ni(II) ions by humans. <b>Methods:</b> Unstimulated whole-mouth human saliva samples were obtained from n = 12 pre-fasted (≥8 h) healthy participants, and clear whole-mouth salivary supernatants (WMSSs) were obtained from these via centrifugation. Microlitre aliquots of stock aqueous Ni(II) solutions were sequentially titrated into WMSS samples via micropipette. Any possible added concentration-dependent Ni(II)-mediated pH changes therein were experimentally controlled. <sup>1</sup>H NMR spectra were acquired on a JEOL JNM-ECZ600R/S1 spectrometer. <b>Results:</b> Univariate and multivariate (MV) metabolomics and MV clustering analyses were conducted in a sequential stepwise manner in order to follow the differential effects of increasing concentrations of added Ni(II). The results acquired showed that important Ni(II)-responsive biomolecules could be clustered into distinguishable patterns on the basis of added concentration-dependent responses of their resonance intensities and line widths. At low added concentrations (71 µmol/L), low-WMSS-level N-donor amino acids (especially histidine) and amines with relatively high stability constants for this paramagnetic metal ion were the most responsive (severe resonance broadenings were observed). However, at higher Ni(II) concentrations (140–670 µmol/L), weaker carboxylate O-donor ligands such as lactate, formate, succinate, and acetate were featured as major Ni(II) ligands, a consequence of their much higher WMSS concentrations, which were sufficient for them to compete for these higher Ni(II) availabilities. From these experiments, the metabolites most affected were found to be histidine ≈ methylamines > taurine ≈ lactate ≈ succinate > formate > acetate ≈ ethanol ≈ glycine ≈ N-acetylneuraminate, although they predominantly comprised carboxylato oxygen donor ligands/chelators at the higher added Ni(II) levels. Removal of the interfering effects arising from the differential biomolecular compositions of the WMSS samples collected from different participants and those from the effects exerted by a first-order interaction effect substantially enhanced the statistical significance of the differences observed between the added Ni(II) levels. The addition of EDTA to Ni(II)-treated WMSS samples successfully reversed these resonance modifications, an observation confirming the transfer of Ni(II) from the above endogenous complexants to this exogenous chelator to form the highly stable diamagnetic octahedral [Ni(II)-EDTA] complex (K<sub>stab</sub> = 1.0 × 10<sup>19</sup> M<sup>−1</sup>). <b>Conclusions:</b> The results acquired demonstrated the value of linking advanced experimental design and multivariate metabolomics/statistical analysis techniques to <sup>1</sup>H NMR analysis for such speciation studies. These provided valuable molecular information regarding the identities of Ni(II) complexes in human saliva, which is relevant to trace metal ion speciation and toxicology, the <i>in vivo</i> corrosion of NiC-MADPs, and the molecular fate of ingested Ni(II) ions in this biofluid. The carcinogenic potential of these low-molecular-mass Ni(II) complexes is discussed. |
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| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-b6fd197733444902a20dfe73117a4ad62025-01-24T13:41:07ZengMDPI AGMetabolites2218-19892024-12-01151410.3390/metabo15010004Speciation of Potentially Carcinogenic Trace Nickel(II) Ion Levels in Human Saliva: A Sequential Metabolomics-Facilitated High-Field <sup>1</sup>H NMR InvestigationKayleigh Hunwin0Georgina Page1Mark Edgar2Mohammed Bhogadia3Martin Grootveld4Leicester School of Pharmacy, De Montfort University, Leicester LE1 9BH, UKLeicester School of Pharmacy, De Montfort University, Leicester LE1 9BH, UKLeicester School of Pharmacy, De Montfort University, Leicester LE1 9BH, UKLeicester School of Pharmacy, De Montfort University, Leicester LE1 9BH, UKLeicester School of Pharmacy, De Montfort University, Leicester LE1 9BH, UK<b>Introduction/Objectives:</b> Since the biological activities and toxicities of ‘foreign’ and/or excess levels of metal ions are predominantly determined by their precise molecular nature, here we have employed high-resolution <sup>1</sup>H NMR analysis to explore the ‘speciation’ of paramagnetic Ni(II) ions in human saliva, a potentially rich source of biomolecular Ni(II)-complexants/chelators. These studies are of relevance to the <i>in vivo</i> corrosion of nickel-containing metal alloy dental prostheses (NiC-MADPs) in addition to the dietary or adverse toxicological intake of Ni(II) ions by humans. <b>Methods:</b> Unstimulated whole-mouth human saliva samples were obtained from n = 12 pre-fasted (≥8 h) healthy participants, and clear whole-mouth salivary supernatants (WMSSs) were obtained from these via centrifugation. Microlitre aliquots of stock aqueous Ni(II) solutions were sequentially titrated into WMSS samples via micropipette. Any possible added concentration-dependent Ni(II)-mediated pH changes therein were experimentally controlled. <sup>1</sup>H NMR spectra were acquired on a JEOL JNM-ECZ600R/S1 spectrometer. <b>Results:</b> Univariate and multivariate (MV) metabolomics and MV clustering analyses were conducted in a sequential stepwise manner in order to follow the differential effects of increasing concentrations of added Ni(II). The results acquired showed that important Ni(II)-responsive biomolecules could be clustered into distinguishable patterns on the basis of added concentration-dependent responses of their resonance intensities and line widths. At low added concentrations (71 µmol/L), low-WMSS-level N-donor amino acids (especially histidine) and amines with relatively high stability constants for this paramagnetic metal ion were the most responsive (severe resonance broadenings were observed). However, at higher Ni(II) concentrations (140–670 µmol/L), weaker carboxylate O-donor ligands such as lactate, formate, succinate, and acetate were featured as major Ni(II) ligands, a consequence of their much higher WMSS concentrations, which were sufficient for them to compete for these higher Ni(II) availabilities. From these experiments, the metabolites most affected were found to be histidine ≈ methylamines > taurine ≈ lactate ≈ succinate > formate > acetate ≈ ethanol ≈ glycine ≈ N-acetylneuraminate, although they predominantly comprised carboxylato oxygen donor ligands/chelators at the higher added Ni(II) levels. Removal of the interfering effects arising from the differential biomolecular compositions of the WMSS samples collected from different participants and those from the effects exerted by a first-order interaction effect substantially enhanced the statistical significance of the differences observed between the added Ni(II) levels. The addition of EDTA to Ni(II)-treated WMSS samples successfully reversed these resonance modifications, an observation confirming the transfer of Ni(II) from the above endogenous complexants to this exogenous chelator to form the highly stable diamagnetic octahedral [Ni(II)-EDTA] complex (K<sub>stab</sub> = 1.0 × 10<sup>19</sup> M<sup>−1</sup>). <b>Conclusions:</b> The results acquired demonstrated the value of linking advanced experimental design and multivariate metabolomics/statistical analysis techniques to <sup>1</sup>H NMR analysis for such speciation studies. These provided valuable molecular information regarding the identities of Ni(II) complexes in human saliva, which is relevant to trace metal ion speciation and toxicology, the <i>in vivo</i> corrosion of NiC-MADPs, and the molecular fate of ingested Ni(II) ions in this biofluid. The carcinogenic potential of these low-molecular-mass Ni(II) complexes is discussed.https://www.mdpi.com/2218-1989/15/1/4speciationnickel(II)nickel(II) complexants and chelatorsNMR analysishuman salivadental implant |
| spellingShingle | Kayleigh Hunwin Georgina Page Mark Edgar Mohammed Bhogadia Martin Grootveld Speciation of Potentially Carcinogenic Trace Nickel(II) Ion Levels in Human Saliva: A Sequential Metabolomics-Facilitated High-Field <sup>1</sup>H NMR Investigation Metabolites speciation nickel(II) nickel(II) complexants and chelators NMR analysis human saliva dental implant |
| title | Speciation of Potentially Carcinogenic Trace Nickel(II) Ion Levels in Human Saliva: A Sequential Metabolomics-Facilitated High-Field <sup>1</sup>H NMR Investigation |
| title_full | Speciation of Potentially Carcinogenic Trace Nickel(II) Ion Levels in Human Saliva: A Sequential Metabolomics-Facilitated High-Field <sup>1</sup>H NMR Investigation |
| title_fullStr | Speciation of Potentially Carcinogenic Trace Nickel(II) Ion Levels in Human Saliva: A Sequential Metabolomics-Facilitated High-Field <sup>1</sup>H NMR Investigation |
| title_full_unstemmed | Speciation of Potentially Carcinogenic Trace Nickel(II) Ion Levels in Human Saliva: A Sequential Metabolomics-Facilitated High-Field <sup>1</sup>H NMR Investigation |
| title_short | Speciation of Potentially Carcinogenic Trace Nickel(II) Ion Levels in Human Saliva: A Sequential Metabolomics-Facilitated High-Field <sup>1</sup>H NMR Investigation |
| title_sort | speciation of potentially carcinogenic trace nickel ii ion levels in human saliva a sequential metabolomics facilitated high field sup 1 sup h nmr investigation |
| topic | speciation nickel(II) nickel(II) complexants and chelators NMR analysis human saliva dental implant |
| url | https://www.mdpi.com/2218-1989/15/1/4 |
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