Identification, Molecular Characterization, and Biology of a Novel <i>Quadrivirus</i> Infecting the Plant Fungus <i>Allocryptovalsa sichuanensis</i>
A novel double-stranded RNA (dsRNA) virus was isolated and described from strain ZZZ210557 of plant endophyte <i>Allocryptovalsa sichuanensis</i>. The dsRNA virus contains four dsRNA segments, dsRNA1 to dsRNA4, with a size range of 3.8 to 5.1 kbp. Each possesses a single large ORF and is...
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| author | Yuxu Xie Xianhong Wang Xiuyuan Sun Fanxing Ren Meng Zhang Chao Xu Qingzhou Ma Yuehua Geng Rui Zang Yashuang Guo |
| author_facet | Yuxu Xie Xianhong Wang Xiuyuan Sun Fanxing Ren Meng Zhang Chao Xu Qingzhou Ma Yuehua Geng Rui Zang Yashuang Guo |
| author_sort | Yuxu Xie |
| collection | DOAJ |
| description | A novel double-stranded RNA (dsRNA) virus was isolated and described from strain ZZZ210557 of plant endophyte <i>Allocryptovalsa sichuanensis</i>. The dsRNA virus contains four dsRNA segments, dsRNA1 to dsRNA4, with a size range of 3.8 to 5.1 kbp. Each possesses a single large ORF and is encapsulated in isometric particles approximately 42–47 nm in diameter. Notably, the dsRNA3 encoded sequence revealed modest similarities to the amino acid sequences of RdRps predicted from the nucleotide sequences of known and suspected members of the family <i>Quadriviridae</i>. Phylogenetic analysis of the putative RdRp with the corresponding proteins of other quadriviruses revealed that the dsRNA virus is a new member belonging to the family <i>Quadriviridae</i>, tentatively named <i>Allocryptovalsa sichuanensis</i> quadrivirus 1 (AsQV1). All four segments of AsQV1 could be successfully cured through ribavirin treatment, whereas it likely has no apparent impact on the morphologies or virulence of the host fungus. This study is the first report of a quadrivirus isolated from the fungus <i>A. sichuanensis</i>, and our results enhance the diversity of the quadrivirus. |
| format | Article |
| id | doaj-art-b6fc32e9eb384360a9cde3602fdebb58 |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-b6fc32e9eb384360a9cde3602fdebb582025-08-20T02:45:42ZengMDPI AGViruses1999-49152025-02-0117227510.3390/v17020275Identification, Molecular Characterization, and Biology of a Novel <i>Quadrivirus</i> Infecting the Plant Fungus <i>Allocryptovalsa sichuanensis</i>Yuxu Xie0Xianhong Wang1Xiuyuan Sun2Fanxing Ren3Meng Zhang4Chao Xu5Qingzhou Ma6Yuehua Geng7Rui Zang8Yashuang Guo9College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaKey Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaA novel double-stranded RNA (dsRNA) virus was isolated and described from strain ZZZ210557 of plant endophyte <i>Allocryptovalsa sichuanensis</i>. The dsRNA virus contains four dsRNA segments, dsRNA1 to dsRNA4, with a size range of 3.8 to 5.1 kbp. Each possesses a single large ORF and is encapsulated in isometric particles approximately 42–47 nm in diameter. Notably, the dsRNA3 encoded sequence revealed modest similarities to the amino acid sequences of RdRps predicted from the nucleotide sequences of known and suspected members of the family <i>Quadriviridae</i>. Phylogenetic analysis of the putative RdRp with the corresponding proteins of other quadriviruses revealed that the dsRNA virus is a new member belonging to the family <i>Quadriviridae</i>, tentatively named <i>Allocryptovalsa sichuanensis</i> quadrivirus 1 (AsQV1). All four segments of AsQV1 could be successfully cured through ribavirin treatment, whereas it likely has no apparent impact on the morphologies or virulence of the host fungus. This study is the first report of a quadrivirus isolated from the fungus <i>A. sichuanensis</i>, and our results enhance the diversity of the quadrivirus.https://www.mdpi.com/1999-4915/17/2/275<i>Allocryptovalsa sichuanensis</i><i>Allocryptovalsa sichuanensis</i> quadrivirus 1dsRNA mycoviruses<i>Quadriviridae</i> |
| spellingShingle | Yuxu Xie Xianhong Wang Xiuyuan Sun Fanxing Ren Meng Zhang Chao Xu Qingzhou Ma Yuehua Geng Rui Zang Yashuang Guo Identification, Molecular Characterization, and Biology of a Novel <i>Quadrivirus</i> Infecting the Plant Fungus <i>Allocryptovalsa sichuanensis</i> Viruses <i>Allocryptovalsa sichuanensis</i> <i>Allocryptovalsa sichuanensis</i> quadrivirus 1 dsRNA mycoviruses <i>Quadriviridae</i> |
| title | Identification, Molecular Characterization, and Biology of a Novel <i>Quadrivirus</i> Infecting the Plant Fungus <i>Allocryptovalsa sichuanensis</i> |
| title_full | Identification, Molecular Characterization, and Biology of a Novel <i>Quadrivirus</i> Infecting the Plant Fungus <i>Allocryptovalsa sichuanensis</i> |
| title_fullStr | Identification, Molecular Characterization, and Biology of a Novel <i>Quadrivirus</i> Infecting the Plant Fungus <i>Allocryptovalsa sichuanensis</i> |
| title_full_unstemmed | Identification, Molecular Characterization, and Biology of a Novel <i>Quadrivirus</i> Infecting the Plant Fungus <i>Allocryptovalsa sichuanensis</i> |
| title_short | Identification, Molecular Characterization, and Biology of a Novel <i>Quadrivirus</i> Infecting the Plant Fungus <i>Allocryptovalsa sichuanensis</i> |
| title_sort | identification molecular characterization and biology of a novel i quadrivirus i infecting the plant fungus i allocryptovalsa sichuanensis i |
| topic | <i>Allocryptovalsa sichuanensis</i> <i>Allocryptovalsa sichuanensis</i> quadrivirus 1 dsRNA mycoviruses <i>Quadriviridae</i> |
| url | https://www.mdpi.com/1999-4915/17/2/275 |
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