Identification, Molecular Characterization, and Biology of a Novel <i>Quadrivirus</i> Infecting the Plant Fungus <i>Allocryptovalsa sichuanensis</i>
A novel double-stranded RNA (dsRNA) virus was isolated and described from strain ZZZ210557 of plant endophyte <i>Allocryptovalsa sichuanensis</i>. The dsRNA virus contains four dsRNA segments, dsRNA1 to dsRNA4, with a size range of 3.8 to 5.1 kbp. Each possesses a single large ORF and is...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
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| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/17/2/275 |
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| Summary: | A novel double-stranded RNA (dsRNA) virus was isolated and described from strain ZZZ210557 of plant endophyte <i>Allocryptovalsa sichuanensis</i>. The dsRNA virus contains four dsRNA segments, dsRNA1 to dsRNA4, with a size range of 3.8 to 5.1 kbp. Each possesses a single large ORF and is encapsulated in isometric particles approximately 42–47 nm in diameter. Notably, the dsRNA3 encoded sequence revealed modest similarities to the amino acid sequences of RdRps predicted from the nucleotide sequences of known and suspected members of the family <i>Quadriviridae</i>. Phylogenetic analysis of the putative RdRp with the corresponding proteins of other quadriviruses revealed that the dsRNA virus is a new member belonging to the family <i>Quadriviridae</i>, tentatively named <i>Allocryptovalsa sichuanensis</i> quadrivirus 1 (AsQV1). All four segments of AsQV1 could be successfully cured through ribavirin treatment, whereas it likely has no apparent impact on the morphologies or virulence of the host fungus. This study is the first report of a quadrivirus isolated from the fungus <i>A. sichuanensis</i>, and our results enhance the diversity of the quadrivirus. |
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| ISSN: | 1999-4915 |