Protocol for mapping 2′-O-methylation using nanopore direct RNA-seq data with NanoNm
Summary: 2'-O-methylation (Nm) is pivotal in rRNA and the internal site of mRNA. Here, we present an integrated bioinformatics protocol for the identification of 2′-O-methylation using nanopore direct RNA sequencing (RNA-seq) data. We describe steps for software installation, data collection, a...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725004095 |
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