Protocol for mapping 2′-O-methylation using nanopore direct RNA-seq data with NanoNm

Protocol for mapping 2′-O-methylation using nanopore direct RNA-seq data with NanoNm

Summary: 2'-O-methylation (Nm) is pivotal in rRNA and the internal site of mRNA. Here, we present an integrated bioinformatics protocol for the identification of 2′-O-methylation using nanopore direct RNA sequencing (RNA-seq) data. We describe steps for software installation, data collection, a...

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Bibliographic Details
Main Authors: Yanqiang Li, Jiayi Chen, Yunxia Wang, Dean Li, Jiahan Li, Yang Yi, Lili Zhang, Qi Cao, Kaifu Chen
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Bioinformatics
Genomics
RNA-seq
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725004095
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