Biochar Alteration of the Sorption of Substrates and Products in Soil Enzyme Assays
Pine wood and barley straw biochar amendments to Kettering and Cameroon sandy silt loam soils (15, 30, or 150 mg biochar g−1 soil) caused significant reductions (up to 80%, P<0.05) in concentrations of substrate and extractable product in soil dehydrogenase and phosphomonoesterase enzyme assays....
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Applied and Environmental Soil Science |
| Online Access: | http://dx.doi.org/10.1155/2013/968682 |
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| _version_ | 1849686193493508096 |
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| author | Mark Swaine Rachel Obrike Joanna M. Clark Liz J. Shaw |
| author_facet | Mark Swaine Rachel Obrike Joanna M. Clark Liz J. Shaw |
| author_sort | Mark Swaine |
| collection | DOAJ |
| description | Pine wood and barley straw biochar amendments to Kettering and Cameroon sandy silt loam soils (15, 30, or 150 mg biochar g−1 soil) caused significant reductions (up to 80%, P<0.05) in concentrations of substrate and extractable product in soil dehydrogenase and phosphomonoesterase enzyme assays. Likely this was caused by increased solid-phase sorption of the chemicals in the presence of the biochars under assay conditions. The relationship between assay chemical sorption and biochar concentration depended on the chemical, soil type, biochar type, and their interactions; hence, no uniform correction factor could be derived. This biochar impact on assay constituents will limit the identification of genuine biochar effects on soil enzymes. It is recommended that the assumption of saturating substrate concentrations be checked and that product standards be matrix-matched when conducting enzyme assays with biochar-amended soil. |
| format | Article |
| id | doaj-art-b6e839b9b0584a1db4e7c814d536a8e7 |
| institution | DOAJ |
| issn | 1687-7667 1687-7675 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applied and Environmental Soil Science |
| spelling | doaj-art-b6e839b9b0584a1db4e7c814d536a8e72025-08-20T03:22:48ZengWileyApplied and Environmental Soil Science1687-76671687-76752013-01-01201310.1155/2013/968682968682Biochar Alteration of the Sorption of Substrates and Products in Soil Enzyme AssaysMark Swaine0Rachel Obrike1Joanna M. Clark2Liz J. Shaw3Soil Research Centre, Department of Geography and Environmental Science, University of Reading, Reading RG6 6DW, UKSoil Research Centre, Department of Geography and Environmental Science, University of Reading, Reading RG6 6DW, UKSoil Research Centre, Department of Geography and Environmental Science, University of Reading, Reading RG6 6DW, UKSoil Research Centre, Department of Geography and Environmental Science, University of Reading, Reading RG6 6DW, UKPine wood and barley straw biochar amendments to Kettering and Cameroon sandy silt loam soils (15, 30, or 150 mg biochar g−1 soil) caused significant reductions (up to 80%, P<0.05) in concentrations of substrate and extractable product in soil dehydrogenase and phosphomonoesterase enzyme assays. Likely this was caused by increased solid-phase sorption of the chemicals in the presence of the biochars under assay conditions. The relationship between assay chemical sorption and biochar concentration depended on the chemical, soil type, biochar type, and their interactions; hence, no uniform correction factor could be derived. This biochar impact on assay constituents will limit the identification of genuine biochar effects on soil enzymes. It is recommended that the assumption of saturating substrate concentrations be checked and that product standards be matrix-matched when conducting enzyme assays with biochar-amended soil.http://dx.doi.org/10.1155/2013/968682 |
| spellingShingle | Mark Swaine Rachel Obrike Joanna M. Clark Liz J. Shaw Biochar Alteration of the Sorption of Substrates and Products in Soil Enzyme Assays Applied and Environmental Soil Science |
| title | Biochar Alteration of the Sorption of Substrates and Products in Soil Enzyme Assays |
| title_full | Biochar Alteration of the Sorption of Substrates and Products in Soil Enzyme Assays |
| title_fullStr | Biochar Alteration of the Sorption of Substrates and Products in Soil Enzyme Assays |
| title_full_unstemmed | Biochar Alteration of the Sorption of Substrates and Products in Soil Enzyme Assays |
| title_short | Biochar Alteration of the Sorption of Substrates and Products in Soil Enzyme Assays |
| title_sort | biochar alteration of the sorption of substrates and products in soil enzyme assays |
| url | http://dx.doi.org/10.1155/2013/968682 |
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