Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography.
Contrast-enhanced micro-computed tomography (CEμCT) with phosphotungstic acid (PTA) has shown potential for detecting collagen distribution of articular cartilage. However, the selectivity of the PTA staining to articular cartilage constituents remains to be elucidated. The aim of this study was to...
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Public Library of Science (PLoS)
2017-01-01
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| author | Sakari S Karhula Mikko A Finnilä Mikko J Lammi Janne H Ylärinne Sami Kauppinen Lassi Rieppo Kenneth P H Pritzker Heikki J Nieminen Simo Saarakkala |
| author_facet | Sakari S Karhula Mikko A Finnilä Mikko J Lammi Janne H Ylärinne Sami Kauppinen Lassi Rieppo Kenneth P H Pritzker Heikki J Nieminen Simo Saarakkala |
| author_sort | Sakari S Karhula |
| collection | DOAJ |
| description | Contrast-enhanced micro-computed tomography (CEμCT) with phosphotungstic acid (PTA) has shown potential for detecting collagen distribution of articular cartilage. However, the selectivity of the PTA staining to articular cartilage constituents remains to be elucidated. The aim of this study was to investigate the dependence of PTA for the collagen content in bovine articular cartilage. Adjacent bovine articular cartilage samples were treated with chondroitinase ABC and collagenase to degrade the proteoglycan and the collagen constituents in articular cartilage, respectively. Enzymatically degraded samples were compared to the untreated samples using CEμCT and reference methods, such as Fourier-transform infrared imaging. Decrease in the X-ray attenuation of PTA in articular cartilage and collagen content was observed in cartilage depth of 0-13% and deeper in tissue after collagen degradation. Increase in the X-ray attenuation of PTA was observed in the cartilage depth of 13-39% after proteoglycan degradation. The X-ray attenuation of PTA-labelled articular cartilage in CEμCT is associated mainly with collagen content but the proteoglycans have a minor effect on the X-ray attenuation of the PTA-labelled articular cartilage. In conclusion, the PTA labeling provides a feasible CEμCT method for 3D characterization of articular cartilage. |
| format | Article |
| id | doaj-art-b6e7484e706343a8bdfaffe542b9b69e |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b6e7484e706343a8bdfaffe542b9b69e2025-08-20T03:24:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01121e017107510.1371/journal.pone.0171075Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography.Sakari S KarhulaMikko A FinniläMikko J LammiJanne H YlärinneSami KauppinenLassi RieppoKenneth P H PritzkerHeikki J NieminenSimo SaarakkalaContrast-enhanced micro-computed tomography (CEμCT) with phosphotungstic acid (PTA) has shown potential for detecting collagen distribution of articular cartilage. However, the selectivity of the PTA staining to articular cartilage constituents remains to be elucidated. The aim of this study was to investigate the dependence of PTA for the collagen content in bovine articular cartilage. Adjacent bovine articular cartilage samples were treated with chondroitinase ABC and collagenase to degrade the proteoglycan and the collagen constituents in articular cartilage, respectively. Enzymatically degraded samples were compared to the untreated samples using CEμCT and reference methods, such as Fourier-transform infrared imaging. Decrease in the X-ray attenuation of PTA in articular cartilage and collagen content was observed in cartilage depth of 0-13% and deeper in tissue after collagen degradation. Increase in the X-ray attenuation of PTA was observed in the cartilage depth of 13-39% after proteoglycan degradation. The X-ray attenuation of PTA-labelled articular cartilage in CEμCT is associated mainly with collagen content but the proteoglycans have a minor effect on the X-ray attenuation of the PTA-labelled articular cartilage. In conclusion, the PTA labeling provides a feasible CEμCT method for 3D characterization of articular cartilage.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171075&type=printable |
| spellingShingle | Sakari S Karhula Mikko A Finnilä Mikko J Lammi Janne H Ylärinne Sami Kauppinen Lassi Rieppo Kenneth P H Pritzker Heikki J Nieminen Simo Saarakkala Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography. PLoS ONE |
| title | Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography. |
| title_full | Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography. |
| title_fullStr | Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography. |
| title_full_unstemmed | Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography. |
| title_short | Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography. |
| title_sort | effects of articular cartilage constituents on phosphotungstic acid enhanced micro computed tomography |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171075&type=printable |
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