<p><strong>Effectiveness of </strong><strong><em>Calotropis procera</em></strong><strong> (Apocynaceae) leaf extract and its fractionations against </strong><strong><em>Tetranychus urticae</em></strong><strong> Koch </strong><strong>(</strong><strong>Acari</strong><strong>: Tetranychidae)</strong></p>
In this study, toxicity of crude leaf extract of Calotropis procera (Aiton) (Apocynaceae) was assessed against Tetranychus urticae Koch (Acari: Tetranychidae) in laboratory conditions using the spraying method. The LC50 values after 24 hours of treatment were calculated as 3608.73 and 2277.02 mg/L...
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Acarological Society of Iran
2024-04-01
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| Series: | Persian Journal of Acarology |
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| Online Access: | https://www.biotaxa.org/pja/article/view/84600 |
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| author | Sherin Hassan Mohammed Safar Makram A. Sayed Doaa F. El Sherif |
| author_facet | Sherin Hassan Mohammed Safar Makram A. Sayed Doaa F. El Sherif |
| author_sort | Sherin Hassan Mohammed Safar |
| collection | DOAJ |
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In this study, toxicity of crude leaf extract of Calotropis procera (Aiton) (Apocynaceae) was assessed against Tetranychus urticae Koch (Acari: Tetranychidae) in laboratory conditions using the spraying method. The LC50 values after 24 hours of treatment were calculated as 3608.73 and 2277.02 mg/L for female and male mites respectively. These amounts after 48 hours of treatment were 2456.98 and 1671.2 mg/L. Results showed that the incubation period, total immature and life cycle durations of T. urticae were prolonged under the influence of the C. procera extract. The fecundity and hatchability percentages were significantly lower (18.2 eggs/females and 94.38%) compared to control (44.73 eggs/females and 99.47%), respectively. The reduction rate of T. urticae at different stages after 1, 3, 7, and 14 days of spraying showed that C. procera had a positive impact on mite population reduction that recorded 94.84 and 100% for females and males 14 days post-treatment. The methanolic extract of C. procera leaves was separated into 18 fractions. The 10th fraction exhibited 67, and 73% mortality, 48 and 72 hours post-treatment, respectively. Seventeen substances were identified in this fraction by using the GC-MS. These findings suggest that the leaf extract of C. procera which contains different novel compounds has the potential to be used for controlling T. urticae in the future.
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| format | Article |
| id | doaj-art-b6e4c84a314c4dabbdc7c7a4a9556a47 |
| institution | Kabale University |
| issn | 2251-8169 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Acarological Society of Iran |
| record_format | Article |
| series | Persian Journal of Acarology |
| spelling | doaj-art-b6e4c84a314c4dabbdc7c7a4a9556a472025-08-20T03:48:23ZengAcarological Society of IranPersian Journal of Acarology2251-81692024-04-0113210.22073/pja.v13i2.84600<p><strong>Effectiveness of </strong><strong><em>Calotropis procera</em></strong><strong> (Apocynaceae) leaf extract and its fractionations against </strong><strong><em>Tetranychus urticae</em></strong><strong> Koch </strong><strong>(</strong><strong>Acari</strong><strong>: Tetranychidae)</strong></p>Sherin Hassan Mohammed Safar0Makram A. Sayed1Doaa F. El Sherif2Department of Plant Protection, Faculty of Agriculture, Fayoum University, Fayoum 63514, EgyptDepartment of Plant Protection, Faculty of Agriculture, Fayoum University, Fayoum 63514, EgyptDepartment of Plant Protection, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt In this study, toxicity of crude leaf extract of Calotropis procera (Aiton) (Apocynaceae) was assessed against Tetranychus urticae Koch (Acari: Tetranychidae) in laboratory conditions using the spraying method. The LC50 values after 24 hours of treatment were calculated as 3608.73 and 2277.02 mg/L for female and male mites respectively. These amounts after 48 hours of treatment were 2456.98 and 1671.2 mg/L. Results showed that the incubation period, total immature and life cycle durations of T. urticae were prolonged under the influence of the C. procera extract. The fecundity and hatchability percentages were significantly lower (18.2 eggs/females and 94.38%) compared to control (44.73 eggs/females and 99.47%), respectively. The reduction rate of T. urticae at different stages after 1, 3, 7, and 14 days of spraying showed that C. procera had a positive impact on mite population reduction that recorded 94.84 and 100% for females and males 14 days post-treatment. The methanolic extract of C. procera leaves was separated into 18 fractions. The 10th fraction exhibited 67, and 73% mortality, 48 and 72 hours post-treatment, respectively. Seventeen substances were identified in this fraction by using the GC-MS. These findings suggest that the leaf extract of C. procera which contains different novel compounds has the potential to be used for controlling T. urticae in the future. https://www.biotaxa.org/pja/article/view/84600Apple of Sodombotanical acaricideGC-MS analysisidentification componentstwo-spotted spider mite |
| spellingShingle | Sherin Hassan Mohammed Safar Makram A. Sayed Doaa F. El Sherif <p><strong>Effectiveness of </strong><strong><em>Calotropis procera</em></strong><strong> (Apocynaceae) leaf extract and its fractionations against </strong><strong><em>Tetranychus urticae</em></strong><strong> Koch </strong><strong>(</strong><strong>Acari</strong><strong>: Tetranychidae)</strong></p> Persian Journal of Acarology Apple of Sodom botanical acaricide GC-MS analysis identification components two-spotted spider mite |
| title | <p><strong>Effectiveness of </strong><strong><em>Calotropis procera</em></strong><strong> (Apocynaceae) leaf extract and its fractionations against </strong><strong><em>Tetranychus urticae</em></strong><strong> Koch </strong><strong>(</strong><strong>Acari</strong><strong>: Tetranychidae)</strong></p> |
| title_full | <p><strong>Effectiveness of </strong><strong><em>Calotropis procera</em></strong><strong> (Apocynaceae) leaf extract and its fractionations against </strong><strong><em>Tetranychus urticae</em></strong><strong> Koch </strong><strong>(</strong><strong>Acari</strong><strong>: Tetranychidae)</strong></p> |
| title_fullStr | <p><strong>Effectiveness of </strong><strong><em>Calotropis procera</em></strong><strong> (Apocynaceae) leaf extract and its fractionations against </strong><strong><em>Tetranychus urticae</em></strong><strong> Koch </strong><strong>(</strong><strong>Acari</strong><strong>: Tetranychidae)</strong></p> |
| title_full_unstemmed | <p><strong>Effectiveness of </strong><strong><em>Calotropis procera</em></strong><strong> (Apocynaceae) leaf extract and its fractionations against </strong><strong><em>Tetranychus urticae</em></strong><strong> Koch </strong><strong>(</strong><strong>Acari</strong><strong>: Tetranychidae)</strong></p> |
| title_short | <p><strong>Effectiveness of </strong><strong><em>Calotropis procera</em></strong><strong> (Apocynaceae) leaf extract and its fractionations against </strong><strong><em>Tetranychus urticae</em></strong><strong> Koch </strong><strong>(</strong><strong>Acari</strong><strong>: Tetranychidae)</strong></p> |
| title_sort | p strong effectiveness of nbsp strong strong em calotropis procera em strong strong apocynaceae leaf extract and its fractionations against strong strong em tetranychus urticae em strong strong koch strong strong strong strong acari strong strong tetranychidae strong p |
| topic | Apple of Sodom botanical acaricide GC-MS analysis identification components two-spotted spider mite |
| url | https://www.biotaxa.org/pja/article/view/84600 |
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