Aspergillus fumigatus ctf1–a novel zinc finger transcription factor involved in azole resistance
Elucidating the mechanisms underlying antifungal resistance in Aspergillus fumigatus, discovering new antifungal targets, and developing drugs to inhibit resistance are the key approaches to treating A. fumigatus infections. Here, we investigated the function of ctf1 (AFUA_1G03800), a gene encoding...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-02-01
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| Series: | Mycology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21501203.2024.2342521 |
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| author | Xiao Gong Heng Zhang Wenxu Cheng Zhangxuan He Tianyan Ma Tian Chen Yi Sun |
| author_facet | Xiao Gong Heng Zhang Wenxu Cheng Zhangxuan He Tianyan Ma Tian Chen Yi Sun |
| author_sort | Xiao Gong |
| collection | DOAJ |
| description | Elucidating the mechanisms underlying antifungal resistance in Aspergillus fumigatus, discovering new antifungal targets, and developing drugs to inhibit resistance are the key approaches to treating A. fumigatus infections. Here, we investigated the function of ctf1 (AFUA_1G03800), a gene encoding a C6 transcription factor. Homologous recombination replacement technology was employed to construct ctf1-knockout and revertant strains. Fungal morphological observations revealed that the growth of the knockout strain was slower, showing fewer conidia. The minimum inhibitory concentration of triazoles was determined by performing the E-test and by using the micro-liquid-based dilution method. The results indicated that ctf1 deletion decreased the susceptibility of A. fumigatus to voriconazole by 2-fold. The decreased antifungal sensitivity of Δctf1 can be attributed to the increased ergosterol content and the overexpression of mdr1, mdr2, and mdr4. Thus, our results on the function of ctf1 contribute to the elucidation of the mechanisms underlying A. fumigatus resistance and the factors associated with A. fumigatus virulence. |
| format | Article |
| id | doaj-art-b6e29671744340cfbea964c790d248fa |
| institution | Kabale University |
| issn | 2150-1203 2150-1211 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycology |
| spelling | doaj-art-b6e29671744340cfbea964c790d248fa2025-02-06T12:16:17ZengTaylor & Francis GroupMycology2150-12032150-12112025-02-0111410.1080/21501203.2024.2342521Aspergillus fumigatus ctf1–a novel zinc finger transcription factor involved in azole resistanceXiao Gong0Heng Zhang1Wenxu Cheng2Zhangxuan He3Tianyan Ma4Tian Chen5Yi Sun6Department of Dermatology, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou, ChinaDepartment of Dermatology, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou, ChinaDepartment of Otolaryngology, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou, ChinaMedical School, Yangtze University, Jingzhou, ChinaMedical School, Yangtze University, Jingzhou, ChinaMedical School, Yangtze University, Jingzhou, ChinaDepartment of Dermatology, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou, ChinaElucidating the mechanisms underlying antifungal resistance in Aspergillus fumigatus, discovering new antifungal targets, and developing drugs to inhibit resistance are the key approaches to treating A. fumigatus infections. Here, we investigated the function of ctf1 (AFUA_1G03800), a gene encoding a C6 transcription factor. Homologous recombination replacement technology was employed to construct ctf1-knockout and revertant strains. Fungal morphological observations revealed that the growth of the knockout strain was slower, showing fewer conidia. The minimum inhibitory concentration of triazoles was determined by performing the E-test and by using the micro-liquid-based dilution method. The results indicated that ctf1 deletion decreased the susceptibility of A. fumigatus to voriconazole by 2-fold. The decreased antifungal sensitivity of Δctf1 can be attributed to the increased ergosterol content and the overexpression of mdr1, mdr2, and mdr4. Thus, our results on the function of ctf1 contribute to the elucidation of the mechanisms underlying A. fumigatus resistance and the factors associated with A. fumigatus virulence.https://www.tandfonline.com/doi/10.1080/21501203.2024.2342521Aspergillus fumigatusgene knockoutctf1voriconazoledrug sensitivityAFUA_1G03800 |
| spellingShingle | Xiao Gong Heng Zhang Wenxu Cheng Zhangxuan He Tianyan Ma Tian Chen Yi Sun Aspergillus fumigatus ctf1–a novel zinc finger transcription factor involved in azole resistance Mycology Aspergillus fumigatus gene knockout ctf1 voriconazole drug sensitivity AFUA_1G03800 |
| title | Aspergillus fumigatus ctf1–a novel zinc finger transcription factor involved in azole resistance |
| title_full | Aspergillus fumigatus ctf1–a novel zinc finger transcription factor involved in azole resistance |
| title_fullStr | Aspergillus fumigatus ctf1–a novel zinc finger transcription factor involved in azole resistance |
| title_full_unstemmed | Aspergillus fumigatus ctf1–a novel zinc finger transcription factor involved in azole resistance |
| title_short | Aspergillus fumigatus ctf1–a novel zinc finger transcription factor involved in azole resistance |
| title_sort | aspergillus fumigatus ctf1 a novel zinc finger transcription factor involved in azole resistance |
| topic | Aspergillus fumigatus gene knockout ctf1 voriconazole drug sensitivity AFUA_1G03800 |
| url | https://www.tandfonline.com/doi/10.1080/21501203.2024.2342521 |
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