Effectiveness of Red Onion Extract (Allium Cepa) on the Elongation of Papillae and Neutrophil Cell in the Gastroesophageal Junction (GEJ) of Sprague Dawley Strain Rats Gastroesophageal Reflux Disease (GERD) Model

Background: Gastroesophageal acid reflux disease (GERD) is a disorder caused by the reflux of stomach acid when the lower esophageal sphincter (LES) relaxes with a prevalence of around 1.03 billion worldwide. One of the supporting examinations carried out to diagnose GERD is a histopathological exam...

Full description

Saved in:
Bibliographic Details
Main Authors: Maria Bernadetta Nadya Blandina, Vetinly ., Tena Djuartina, Riki Tenggara, Iskandar R. Budianto
Format: Article
Language:English
Published: Interna Publishing 2025-04-01
Series:The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy
Subjects:
Online Access:https://ina-jghe.com/index.php/jghe/article/view/1052
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849309970941607936
author Maria Bernadetta Nadya Blandina
Vetinly .
Tena Djuartina
Riki Tenggara
Iskandar R. Budianto
author_facet Maria Bernadetta Nadya Blandina
Vetinly .
Tena Djuartina
Riki Tenggara
Iskandar R. Budianto
author_sort Maria Bernadetta Nadya Blandina
collection DOAJ
description Background: Gastroesophageal acid reflux disease (GERD) is a disorder caused by the reflux of stomach acid when the lower esophageal sphincter (LES) relaxes with a prevalence of around 1.03 billion worldwide. One of the supporting examinations carried out to diagnose GERD is a histopathological examination. This study aims to determine the effect of red onion extract on the papillae elongation and neutrophil cells of the gastroesophageal junction in Sprague Dawley rats. Methods: This experimental study was conducted on Sprague Dawley rats induced with 1 mL of HCl + 1 mL of ascorbic acid, administered into the gastric cardia of the rats, mixed with 1 mL of sodium thiocyanate (NaSCN) and 1.5 mL of sodium nitrite (NaNO₃) to develop a GERD model. The rats were then divided into ten groups with different treatments, including red onion extract (250, 500, 750 mg/kgBW) and PPI (pantoprazole). Results:  It was stated that there was a significant relationship between the effect of red onions and changes in papillae elongation in rats induced by GERD (P-value 0.05). The neutrophil cells showed no significance on the impact of red onion (p-value 0,05). Nor are the papillae elongation and the neutrophil cell changes dose-dependent. Conclusion: A significant reduction in the histopathological appearance of papillae elongation was seen in the majority of the population. However, no significant results were found for the different doses of shallot extract. Keywords: Gastroesophageal acid reflux disease, allium cepa, papillae elongation, red onion extract, neutrophil cells
format Article
id doaj-art-b6dfb6e86f9e4833a8a1265f01dbabf7
institution Kabale University
issn 1411-4801
2302-8181
language English
publishDate 2025-04-01
publisher Interna Publishing
record_format Article
series The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy
spelling doaj-art-b6dfb6e86f9e4833a8a1265f01dbabf72025-08-20T03:53:56ZengInterna PublishingThe Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy1411-48012302-81812025-04-01261364210.24871/261202536-42915Effectiveness of Red Onion Extract (Allium Cepa) on the Elongation of Papillae and Neutrophil Cell in the Gastroesophageal Junction (GEJ) of Sprague Dawley Strain Rats Gastroesophageal Reflux Disease (GERD) ModelMaria Bernadetta Nadya Blandina0Vetinly .1Tena Djuartina2Riki Tenggara3Iskandar R. Budianto4Faculty of Medicine and Health Sciences of Atma Jaya University, Jakarta, IndonesiaDepartment of public health and nutrition sciences of Atma Jaya University, Jakarta, IndonesiaAnatomy department of Atma Jaya University, Jakarta, IndonesiaInternal medicine department of Atma Jaya University, Jakarta, IndonesiaSurgery department of Atma Jaya university, Jakarta, IndonesiaBackground: Gastroesophageal acid reflux disease (GERD) is a disorder caused by the reflux of stomach acid when the lower esophageal sphincter (LES) relaxes with a prevalence of around 1.03 billion worldwide. One of the supporting examinations carried out to diagnose GERD is a histopathological examination. This study aims to determine the effect of red onion extract on the papillae elongation and neutrophil cells of the gastroesophageal junction in Sprague Dawley rats. Methods: This experimental study was conducted on Sprague Dawley rats induced with 1 mL of HCl + 1 mL of ascorbic acid, administered into the gastric cardia of the rats, mixed with 1 mL of sodium thiocyanate (NaSCN) and 1.5 mL of sodium nitrite (NaNO₃) to develop a GERD model. The rats were then divided into ten groups with different treatments, including red onion extract (250, 500, 750 mg/kgBW) and PPI (pantoprazole). Results:  It was stated that there was a significant relationship between the effect of red onions and changes in papillae elongation in rats induced by GERD (P-value 0.05). The neutrophil cells showed no significance on the impact of red onion (p-value 0,05). Nor are the papillae elongation and the neutrophil cell changes dose-dependent. Conclusion: A significant reduction in the histopathological appearance of papillae elongation was seen in the majority of the population. However, no significant results were found for the different doses of shallot extract. Keywords: Gastroesophageal acid reflux disease, allium cepa, papillae elongation, red onion extract, neutrophil cellshttps://ina-jghe.com/index.php/jghe/article/view/1052gastroesophageal acid reflux disease, allium cepa, papillae elongation, red onion extract, neutrophil cells
spellingShingle Maria Bernadetta Nadya Blandina
Vetinly .
Tena Djuartina
Riki Tenggara
Iskandar R. Budianto
Effectiveness of Red Onion Extract (Allium Cepa) on the Elongation of Papillae and Neutrophil Cell in the Gastroesophageal Junction (GEJ) of Sprague Dawley Strain Rats Gastroesophageal Reflux Disease (GERD) Model
The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy
gastroesophageal acid reflux disease, allium cepa, papillae elongation, red onion extract, neutrophil cells
title Effectiveness of Red Onion Extract (Allium Cepa) on the Elongation of Papillae and Neutrophil Cell in the Gastroesophageal Junction (GEJ) of Sprague Dawley Strain Rats Gastroesophageal Reflux Disease (GERD) Model
title_full Effectiveness of Red Onion Extract (Allium Cepa) on the Elongation of Papillae and Neutrophil Cell in the Gastroesophageal Junction (GEJ) of Sprague Dawley Strain Rats Gastroesophageal Reflux Disease (GERD) Model
title_fullStr Effectiveness of Red Onion Extract (Allium Cepa) on the Elongation of Papillae and Neutrophil Cell in the Gastroesophageal Junction (GEJ) of Sprague Dawley Strain Rats Gastroesophageal Reflux Disease (GERD) Model
title_full_unstemmed Effectiveness of Red Onion Extract (Allium Cepa) on the Elongation of Papillae and Neutrophil Cell in the Gastroesophageal Junction (GEJ) of Sprague Dawley Strain Rats Gastroesophageal Reflux Disease (GERD) Model
title_short Effectiveness of Red Onion Extract (Allium Cepa) on the Elongation of Papillae and Neutrophil Cell in the Gastroesophageal Junction (GEJ) of Sprague Dawley Strain Rats Gastroesophageal Reflux Disease (GERD) Model
title_sort effectiveness of red onion extract allium cepa on the elongation of papillae and neutrophil cell in the gastroesophageal junction gej of sprague dawley strain rats gastroesophageal reflux disease gerd model
topic gastroesophageal acid reflux disease, allium cepa, papillae elongation, red onion extract, neutrophil cells
url https://ina-jghe.com/index.php/jghe/article/view/1052
work_keys_str_mv AT mariabernadettanadyablandina effectivenessofredonionextractalliumcepaontheelongationofpapillaeandneutrophilcellinthegastroesophagealjunctiongejofspraguedawleystrainratsgastroesophagealrefluxdiseasegerdmodel
AT vetinly effectivenessofredonionextractalliumcepaontheelongationofpapillaeandneutrophilcellinthegastroesophagealjunctiongejofspraguedawleystrainratsgastroesophagealrefluxdiseasegerdmodel
AT tenadjuartina effectivenessofredonionextractalliumcepaontheelongationofpapillaeandneutrophilcellinthegastroesophagealjunctiongejofspraguedawleystrainratsgastroesophagealrefluxdiseasegerdmodel
AT rikitenggara effectivenessofredonionextractalliumcepaontheelongationofpapillaeandneutrophilcellinthegastroesophagealjunctiongejofspraguedawleystrainratsgastroesophagealrefluxdiseasegerdmodel
AT iskandarrbudianto effectivenessofredonionextractalliumcepaontheelongationofpapillaeandneutrophilcellinthegastroesophagealjunctiongejofspraguedawleystrainratsgastroesophagealrefluxdiseasegerdmodel