A chromosome-scale genome assembly of the leaf roller, Eumorphobotys obscuralis (Lepidoptera: Pyralidae)

Abstract The leaf roller, Eumorphobotys obscuralis, is a highly destructive pest that causes severe defoliation, ultimately reducing plant vigor, leading to culm death, and resulting in significant economic and ecological losses. Despite its substantial impact, effective prevention and control strat...

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Main Authors: Shu-Zhen Wang, Jun-Ran Zhao, Yu-Mei Dong, Wei Zhang, Yue Ying, Jin-Ping Shu, Jin-Ming Zhang, Peng-Jun Zhang
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05411-z
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author Shu-Zhen Wang
Jun-Ran Zhao
Yu-Mei Dong
Wei Zhang
Yue Ying
Jin-Ping Shu
Jin-Ming Zhang
Peng-Jun Zhang
author_facet Shu-Zhen Wang
Jun-Ran Zhao
Yu-Mei Dong
Wei Zhang
Yue Ying
Jin-Ping Shu
Jin-Ming Zhang
Peng-Jun Zhang
author_sort Shu-Zhen Wang
collection DOAJ
description Abstract The leaf roller, Eumorphobotys obscuralis, is a highly destructive pest that causes severe defoliation, ultimately reducing plant vigor, leading to culm death, and resulting in significant economic and ecological losses. Despite its substantial impact, effective prevention and control strategies remain lacking, further exacerbating the damage caused by this species. To address the critical gap in genomic resources, we generated a high-quality chromosome-scale genome assembly for E. obscuralis using a combination of Illumina short reads, PacBio HiFi long reads, and Hi-C chromatin interaction data. The final assembly spans 701.9 Mb across 31 chromosomes, including the single sex chromosome Z. Key assembly metrics include a contig N50 of 1.73 Mb and a scaffold N50 of 23.70 Mb, with an estimated genome completeness of 96.7%. Genome annotation identified 15,855 protein-coding genes and revealed that repetitive sequences account for 58.9% of the genome. This genomic resource lays a solid foundation for exploring the genetic mechanisms underlying the pest’s biology and adaptation, and serves as a valuable reference for the development of targeted pest management strategies.
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issn 2052-4463
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publishDate 2025-06-01
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spelling doaj-art-b6daa1b10ca64cb09c18f440b579ce212025-08-20T02:35:39ZengNature PortfolioScientific Data2052-44632025-06-011211710.1038/s41597-025-05411-zA chromosome-scale genome assembly of the leaf roller, Eumorphobotys obscuralis (Lepidoptera: Pyralidae)Shu-Zhen Wang0Jun-Ran Zhao1Yu-Mei Dong2Wei Zhang3Yue Ying4Jin-Ping Shu5Jin-Ming Zhang6Peng-Jun Zhang7College of Life and Environmental Sciences, Hangzhou Normal UniversityCollege of Life and Environmental Sciences, Hangzhou Normal UniversityCollege of Life and Environmental Sciences, Hangzhou Normal UniversityResearch Institute of Subtropical Forestry, Chinese Academy of ForestryResearch Institute of Subtropical Forestry, Chinese Academy of ForestryResearch Institute of Subtropical Forestry, Chinese Academy of ForestryInstitute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural SciencesCollege of Life and Environmental Sciences, Hangzhou Normal UniversityAbstract The leaf roller, Eumorphobotys obscuralis, is a highly destructive pest that causes severe defoliation, ultimately reducing plant vigor, leading to culm death, and resulting in significant economic and ecological losses. Despite its substantial impact, effective prevention and control strategies remain lacking, further exacerbating the damage caused by this species. To address the critical gap in genomic resources, we generated a high-quality chromosome-scale genome assembly for E. obscuralis using a combination of Illumina short reads, PacBio HiFi long reads, and Hi-C chromatin interaction data. The final assembly spans 701.9 Mb across 31 chromosomes, including the single sex chromosome Z. Key assembly metrics include a contig N50 of 1.73 Mb and a scaffold N50 of 23.70 Mb, with an estimated genome completeness of 96.7%. Genome annotation identified 15,855 protein-coding genes and revealed that repetitive sequences account for 58.9% of the genome. This genomic resource lays a solid foundation for exploring the genetic mechanisms underlying the pest’s biology and adaptation, and serves as a valuable reference for the development of targeted pest management strategies.https://doi.org/10.1038/s41597-025-05411-z
spellingShingle Shu-Zhen Wang
Jun-Ran Zhao
Yu-Mei Dong
Wei Zhang
Yue Ying
Jin-Ping Shu
Jin-Ming Zhang
Peng-Jun Zhang
A chromosome-scale genome assembly of the leaf roller, Eumorphobotys obscuralis (Lepidoptera: Pyralidae)
Scientific Data
title A chromosome-scale genome assembly of the leaf roller, Eumorphobotys obscuralis (Lepidoptera: Pyralidae)
title_full A chromosome-scale genome assembly of the leaf roller, Eumorphobotys obscuralis (Lepidoptera: Pyralidae)
title_fullStr A chromosome-scale genome assembly of the leaf roller, Eumorphobotys obscuralis (Lepidoptera: Pyralidae)
title_full_unstemmed A chromosome-scale genome assembly of the leaf roller, Eumorphobotys obscuralis (Lepidoptera: Pyralidae)
title_short A chromosome-scale genome assembly of the leaf roller, Eumorphobotys obscuralis (Lepidoptera: Pyralidae)
title_sort chromosome scale genome assembly of the leaf roller eumorphobotys obscuralis lepidoptera pyralidae
url https://doi.org/10.1038/s41597-025-05411-z
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