High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb
Plant roots can be manipulated by Agrobacterium rhizogenes to stimulate the production of heterologous proteins for pharmaceutical applications as green cell-factories. During the present study, four bacterial strains (A4, ATCC15834, ATCC11325 and A13) in combination with three co-cultivation media...
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2016-10-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/12681 |
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| author | Sara Kabirnataj Ghorbanali Nematzadeh Jafar Zolala Ahmad Farhad Talebi |
| author_facet | Sara Kabirnataj Ghorbanali Nematzadeh Jafar Zolala Ahmad Farhad Talebi |
| author_sort | Sara Kabirnataj |
| collection | DOAJ |
| description | Plant roots can be manipulated by Agrobacterium rhizogenes to stimulate the production of heterologous proteins for pharmaceutical applications as green cell-factories. During the present study, four bacterial strains (A4, ATCC15834, ATCC11325 and A13) in combination with three co-cultivation media (MS, B5, LS) were examined to establish an efficient and reliable transformation system for chicory (Cichorium intybus L.) using A. rhizogenes. The maximum chicory hairy roots induction was achieved using A13 strain. The observation confirmed that MS medium was more effective on hairy root growth. Dried biomass accumulation of hairy roots infected by A13 strain was 1.10 g l-1 in MS medium which was significantly higher than those grown in LS and B5 medium (0.88 and 0.72 g l-1, respectively). Beta-glucuronidase (GUS) gene was introduced by A13 strain carrying the pCAMBIA1304 binary vector. The results showed that the highest frequency of transformation (63.15 %) was achieved using A13 strain and MS cultivation medium. Detection of GUS and hptII genes by PCR and GUS histochemical localization confirmed the integrative transformation in hairy roots. In conclusion, the whole process was successfully optimized as a pre-step to manipulate the chicory hairy root cells to improve the unique potential of secondary metabolite production. |
| format | Article |
| id | doaj-art-b6d8d6c8dd2d43148eae38d8f17440b8 |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 2016-10-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-b6d8d6c8dd2d43148eae38d8f17440b82025-08-20T02:57:36ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412016-10-01107232133410.14720/aas.2016.107.2.0619073High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herbSara Kabirnataj0Ghorbanali Nematzadeh1Jafar Zolala2Ahmad Farhad Talebi3Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, IranGenetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, IranDepartment of Plant Biotechnology, Faculty of Agriculture, Shahid Bahonar University of Kerman, IranDepartment of Genetics, Faculty of Microbial Biotechnology, Semnan University, IranPlant roots can be manipulated by Agrobacterium rhizogenes to stimulate the production of heterologous proteins for pharmaceutical applications as green cell-factories. During the present study, four bacterial strains (A4, ATCC15834, ATCC11325 and A13) in combination with three co-cultivation media (MS, B5, LS) were examined to establish an efficient and reliable transformation system for chicory (Cichorium intybus L.) using A. rhizogenes. The maximum chicory hairy roots induction was achieved using A13 strain. The observation confirmed that MS medium was more effective on hairy root growth. Dried biomass accumulation of hairy roots infected by A13 strain was 1.10 g l-1 in MS medium which was significantly higher than those grown in LS and B5 medium (0.88 and 0.72 g l-1, respectively). Beta-glucuronidase (GUS) gene was introduced by A13 strain carrying the pCAMBIA1304 binary vector. The results showed that the highest frequency of transformation (63.15 %) was achieved using A13 strain and MS cultivation medium. Detection of GUS and hptII genes by PCR and GUS histochemical localization confirmed the integrative transformation in hairy roots. In conclusion, the whole process was successfully optimized as a pre-step to manipulate the chicory hairy root cells to improve the unique potential of secondary metabolite production.https://journals.uni-lj.si/aas/article/view/12681chicorya. rhizogeneshairy rootgusa13 |
| spellingShingle | Sara Kabirnataj Ghorbanali Nematzadeh Jafar Zolala Ahmad Farhad Talebi High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb Acta Agriculturae Slovenica chicory a. rhizogenes hairy root gus a13 |
| title | High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb |
| title_full | High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb |
| title_fullStr | High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb |
| title_full_unstemmed | High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb |
| title_short | High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb |
| title_sort | high efficient transgenic hairy roots induction in chicory re dawn of a traditional herb |
| topic | chicory a. rhizogenes hairy root gus a13 |
| url | https://journals.uni-lj.si/aas/article/view/12681 |
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