Callus induction and high-frequency plant regeneration for stem apex of Polygonum cuspidatum
The regeneration system of stem apex of Polygonum cuspidatum in vitro was studied with stem apex as explants. The effects of seedling age, inoculation method, different plant growth regulators, AgNO 3 and sucrose etc on callus induction, bud differentiation and rooting of stem apex of Polygonum cusp...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2008-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.05.007 |
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| Summary: | The regeneration system of stem apex of Polygonum cuspidatum in vitro was studied with stem apex as explants. The effects of seedling age, inoculation method, different plant growth regulators, AgNO 3 and sucrose etc on callus induction, bud differentiation and rooting of stem apex of Polygonum cuspidatum were investigated. The results show the optimal medium for callus induction was MS + sucrose 28 g·L<sup>-1</sup> + agar 5.5 g·L<sup>-1</sup> + 2, 4-D 1.5 mg·L<sup>-1</sup> + 6-BA 1.0 mg·L<sup>-1</sup>, and the induction rate was 100%; there was no significant difference on callus induction ability of stem apex from 3 to 7 days of seedling age, callus average induction rate was up to 95%. With the seedling age increasing, callus induction ability reduced quickly, and induction rate was only 55.6% on 12 days. Callus induction rate was higher on stem apex inoculated with their abaxial ends up than down or placed horizontally. The best medium for bud differentiation was MS + AgNO<sub>3</sub> 4.0 mg·L<sup>-1</sup> + sucrose 30 g·L<sup>-1</sup> + agar 6.0 g·L<sup>-1</sup> + NAA 0.5 mg·L<sup>-1</sup> + TDZ 0.8 mg·L<sup>-1</sup>, and the bud differentiation rate and multiplying coefficient were 83.9% and 7.63, respectively. The rooting medium was 1/2 MS + sucrose 26 g·L<sup>-1</sup> + agar 6.5 g·L<sup>-1</sup> + activated carbon 3% + IBA 0.2 mg·L<sup>-1</sup> + NAA 0.3 mg·L<sup>-1</sup>, and the rooting rate was 89.6%. In root induction culture, the rate of root formation with membrane filter was higher (100%) than that with common membrane. |
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| ISSN: | 1008-9209 2097-5155 |