From 19th-century mysteries to modern insights: untangling Aplectana membranosa from Brazilian anurans

Aplectana membranosa is a cosmocercid nematode that shows affinity with various amphibian and reptile hosts, being considered a generalist species. To date, no studies have investigated the influence of host and locality in the morphological variation of this species. Thus, we analysed morphological...

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Main Authors: Ana Nunes Santos, Evelyn Lebrego Cardoso, Lorena Freitas Souza Tavares-Costa, Rayline Thaimenne Alves Figueredo, Gabriel Lima Rebêlo, Maria Isabel Müller, Edna P. Alcantara, Edson A. Adriano, Drausio Honorio Morais, Simone Mousinho Freire, Jeannie Nascimento Dos Santos, Francisco Tiago de Vasconcelos Melo
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Language:English
Published: Cambridge University Press
Series:Parasitology
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Online Access:https://www.cambridge.org/core/product/identifier/S0031182025000204/type/journal_article
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author Ana Nunes Santos
Evelyn Lebrego Cardoso
Lorena Freitas Souza Tavares-Costa
Rayline Thaimenne Alves Figueredo
Gabriel Lima Rebêlo
Maria Isabel Müller
Edna P. Alcantara
Edson A. Adriano
Drausio Honorio Morais
Simone Mousinho Freire
Jeannie Nascimento Dos Santos
Francisco Tiago de Vasconcelos Melo
author_facet Ana Nunes Santos
Evelyn Lebrego Cardoso
Lorena Freitas Souza Tavares-Costa
Rayline Thaimenne Alves Figueredo
Gabriel Lima Rebêlo
Maria Isabel Müller
Edna P. Alcantara
Edson A. Adriano
Drausio Honorio Morais
Simone Mousinho Freire
Jeannie Nascimento Dos Santos
Francisco Tiago de Vasconcelos Melo
author_sort Ana Nunes Santos
collection DOAJ
description Aplectana membranosa is a cosmocercid nematode that shows affinity with various amphibian and reptile hosts, being considered a generalist species. To date, no studies have investigated the influence of host and locality in the morphological variation of this species. Thus, we analysed morphological and morphometric characters of 260 specimens of A. membranosa collected from 9 host species and 7 different localities. To complement the metric studies, we conducted phylogenetic analyses using the ribosomal genes 28S and internal transcript spacer 1 (ITS1) to determine the phylogenetic position of the species and its divergence. In the present study, it was possible to observe the cloacal papillae pattern of the species through scanning electron microscopy, and we found no morphological variation in the specimens of A. membranosa from various hosts in different localities in Brazil. The study showed low variation in all data. However, despite the low variation, we found that external environmental conditions, such as climate and latitude, influence its variation. Molecular analyses highlighted that the separation of Cosmocercidae members may be related to geographic distribution and population genetic divergence. Thus, the results illustrated in this study reiterate the importance of using integrative data to better elucidate the family’s taxonomic and evolutionary history.
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spelling doaj-art-b6c051840ba74b10b7761d99c8ff49182025-08-20T03:01:51ZengCambridge University PressParasitology0031-18201469-816112410.1017/S0031182025000204From 19th-century mysteries to modern insights: untangling Aplectana membranosa from Brazilian anuransAna Nunes Santos0https://orcid.org/0000-0001-6552-2756Evelyn Lebrego Cardoso1https://orcid.org/0000-0002-7155-4559Lorena Freitas Souza Tavares-Costa2https://orcid.org/0000-0002-7518-628XRayline Thaimenne Alves Figueredo3https://orcid.org/0000-0003-4100-9039Gabriel Lima Rebêlo4https://orcid.org/0000-0003-2131-2268Maria Isabel Müller5https://orcid.org/0000-0003-3407-8335Edna P. Alcantara6https://orcid.org/0000-0002-7814-531XEdson A. Adriano7https://orcid.org/0000-0002-6903-9531Drausio Honorio Morais8https://orcid.org/0000-0002-9866-6008Simone Mousinho Freire9https://orcid.org/0000-0001-6417-3144Jeannie Nascimento Dos Santos10https://orcid.org/0000-0002-6612-6410Francisco Tiago de Vasconcelos Melo11https://orcid.org/0000-0001-8935-2923Laboratório de Biologia Celular e Helmintologia ‘Profa Dra Reinalda Marisa Lanfredi’ Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, BrasilLaboratório de Biologia Celular e Helmintologia ‘Profa Dra Reinalda Marisa Lanfredi’ Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, BrasilLaboratório de Biologia Celular e Helmintologia ‘Profa Dra Reinalda Marisa Lanfredi’ Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, BrasilDepartamento de Biologia Animal, Universidade Estadual de Campinas-UNICAMP, Campinas, SP, BrasilLaboratório de Biologia Celular e Helmintologia ‘Profa Dra Reinalda Marisa Lanfredi’ Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, BrasilInstituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, SP, Brasil Oregon State University, Department of Microbiology, Corvallis, OR, USAInstituto de Ciências Agrárias, Universidade Federal de Uberlândia (UFU), Monte Carmelo, MG, BrasilDepartamento de Biologia Animal, Universidade Estadual de Campinas-UNICAMP, Campinas, SP, Brasil Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, SP, BrasilInstituto de Ciências Agrárias, Universidade Federal de Uberlândia (UFU), Monte Carmelo, MG, BrasilDepartamento de Biologia, Laboratório de Zoologia e Biologia Parasitária, Universidade Estadual do Piauí, Teresina, PI, BrasilLaboratório de Biologia Celular e Helmintologia ‘Profa Dra Reinalda Marisa Lanfredi’ Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, BrasilLaboratório de Biologia Celular e Helmintologia ‘Profa Dra Reinalda Marisa Lanfredi’ Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, BrasilAplectana membranosa is a cosmocercid nematode that shows affinity with various amphibian and reptile hosts, being considered a generalist species. To date, no studies have investigated the influence of host and locality in the morphological variation of this species. Thus, we analysed morphological and morphometric characters of 260 specimens of A. membranosa collected from 9 host species and 7 different localities. To complement the metric studies, we conducted phylogenetic analyses using the ribosomal genes 28S and internal transcript spacer 1 (ITS1) to determine the phylogenetic position of the species and its divergence. In the present study, it was possible to observe the cloacal papillae pattern of the species through scanning electron microscopy, and we found no morphological variation in the specimens of A. membranosa from various hosts in different localities in Brazil. The study showed low variation in all data. However, despite the low variation, we found that external environmental conditions, such as climate and latitude, influence its variation. Molecular analyses highlighted that the separation of Cosmocercidae members may be related to geographic distribution and population genetic divergence. Thus, the results illustrated in this study reiterate the importance of using integrative data to better elucidate the family’s taxonomic and evolutionary history.https://www.cambridge.org/core/product/identifier/S0031182025000204/type/journal_articleAplectana membranosaintegrative taxonomymetric variationpopulation divergence
spellingShingle Ana Nunes Santos
Evelyn Lebrego Cardoso
Lorena Freitas Souza Tavares-Costa
Rayline Thaimenne Alves Figueredo
Gabriel Lima Rebêlo
Maria Isabel Müller
Edna P. Alcantara
Edson A. Adriano
Drausio Honorio Morais
Simone Mousinho Freire
Jeannie Nascimento Dos Santos
Francisco Tiago de Vasconcelos Melo
From 19th-century mysteries to modern insights: untangling Aplectana membranosa from Brazilian anurans
Parasitology
Aplectana membranosa
integrative taxonomy
metric variation
population divergence
title From 19th-century mysteries to modern insights: untangling Aplectana membranosa from Brazilian anurans
title_full From 19th-century mysteries to modern insights: untangling Aplectana membranosa from Brazilian anurans
title_fullStr From 19th-century mysteries to modern insights: untangling Aplectana membranosa from Brazilian anurans
title_full_unstemmed From 19th-century mysteries to modern insights: untangling Aplectana membranosa from Brazilian anurans
title_short From 19th-century mysteries to modern insights: untangling Aplectana membranosa from Brazilian anurans
title_sort from 19th century mysteries to modern insights untangling aplectana membranosa from brazilian anurans
topic Aplectana membranosa
integrative taxonomy
metric variation
population divergence
url https://www.cambridge.org/core/product/identifier/S0031182025000204/type/journal_article
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