Genome-wide association analysis and gene mining of flavonoids in Xanthoceras sorbifolia
Abstract Xanthoceras sorbifolia is a unique woody oilseed tree in China, and its leaves are rich in flavonoids, which are involved in plant growth, development and defense. However, the mining of flavonoid synthesis-related genes in Xanthoceras sorbifolia leaves is lacking. In this study, 226 leaves...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-00514-4 |
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| author | Yuxue Huo Lei Wang Lu Lu Dan Wu Li Liu Xiaoman Xie Yongjun Zhao |
| author_facet | Yuxue Huo Lei Wang Lu Lu Dan Wu Li Liu Xiaoman Xie Yongjun Zhao |
| author_sort | Yuxue Huo |
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| description | Abstract Xanthoceras sorbifolia is a unique woody oilseed tree in China, and its leaves are rich in flavonoids, which are involved in plant growth, development and defense. However, the mining of flavonoid synthesis-related genes in Xanthoceras sorbifolia leaves is lacking. In this study, 226 leaves of Xanthoceras sorbifolia from eight provinces in the key distribution areas were measured for flavonoid content, and the differences in flavonoid content of Xanthoceras sorbifolia were analysed to screen out excellent seed sources and six excellent single plants with higher flavonoid content. Genome-wide association analysis (GWAS) was used to identify genes controlling the synthesis of flavonoids, and 62 significant Single nucleotide polymorphism (SNP) sites were identified, which were closely associated with 8 traits, and a total of 11 genes coding for proteins. We found that these genes mainly encode proteins such as WPP domain-associated protein (WAP) (Fragment), Protein pleiotropic regulatory locus 1 (PRL1) and Phosphomevalonate kinase, peroxisomal (PMK), etc. We found that these proteins may directly or indirectly affect the synthesis of flavonoids, which will provide a data base for molecular breeding and genetic improvement of Xanthoceras sorbifolia. |
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| id | doaj-art-b6be7f1674e047ad950baf05db629413 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-b6be7f1674e047ad950baf05db6294132025-08-20T04:01:24ZengNature PortfolioScientific Reports2045-23222025-07-0115111410.1038/s41598-025-00514-4Genome-wide association analysis and gene mining of flavonoids in Xanthoceras sorbifoliaYuxue Huo0Lei Wang1Lu Lu2Dan Wu3Li Liu4Xiaoman Xie5Yongjun Zhao6Key Laboratory of National Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources, Shandong Provincial Center of Forest and Grass Germplasm ResourcesKey Laboratory of National Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources, Shandong Provincial Center of Forest and Grass Germplasm ResourcesKey Laboratory of National Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources, Shandong Provincial Center of Forest and Grass Germplasm ResourcesKey Laboratory of National Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources, Shandong Provincial Center of Forest and Grass Germplasm ResourcesKey Laboratory of National Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources, Shandong Provincial Center of Forest and Grass Germplasm ResourcesKey Laboratory of National Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources, Shandong Provincial Center of Forest and Grass Germplasm ResourcesKey Laboratory of National Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources, Shandong Provincial Center of Forest and Grass Germplasm ResourcesAbstract Xanthoceras sorbifolia is a unique woody oilseed tree in China, and its leaves are rich in flavonoids, which are involved in plant growth, development and defense. However, the mining of flavonoid synthesis-related genes in Xanthoceras sorbifolia leaves is lacking. In this study, 226 leaves of Xanthoceras sorbifolia from eight provinces in the key distribution areas were measured for flavonoid content, and the differences in flavonoid content of Xanthoceras sorbifolia were analysed to screen out excellent seed sources and six excellent single plants with higher flavonoid content. Genome-wide association analysis (GWAS) was used to identify genes controlling the synthesis of flavonoids, and 62 significant Single nucleotide polymorphism (SNP) sites were identified, which were closely associated with 8 traits, and a total of 11 genes coding for proteins. We found that these genes mainly encode proteins such as WPP domain-associated protein (WAP) (Fragment), Protein pleiotropic regulatory locus 1 (PRL1) and Phosphomevalonate kinase, peroxisomal (PMK), etc. We found that these proteins may directly or indirectly affect the synthesis of flavonoids, which will provide a data base for molecular breeding and genetic improvement of Xanthoceras sorbifolia.https://doi.org/10.1038/s41598-025-00514-4Xanthoceras sorbifoliaFlavonoidsWhole genome resequencingSNP screeningGWAS |
| spellingShingle | Yuxue Huo Lei Wang Lu Lu Dan Wu Li Liu Xiaoman Xie Yongjun Zhao Genome-wide association analysis and gene mining of flavonoids in Xanthoceras sorbifolia Scientific Reports Xanthoceras sorbifolia Flavonoids Whole genome resequencing SNP screening GWAS |
| title | Genome-wide association analysis and gene mining of flavonoids in Xanthoceras sorbifolia |
| title_full | Genome-wide association analysis and gene mining of flavonoids in Xanthoceras sorbifolia |
| title_fullStr | Genome-wide association analysis and gene mining of flavonoids in Xanthoceras sorbifolia |
| title_full_unstemmed | Genome-wide association analysis and gene mining of flavonoids in Xanthoceras sorbifolia |
| title_short | Genome-wide association analysis and gene mining of flavonoids in Xanthoceras sorbifolia |
| title_sort | genome wide association analysis and gene mining of flavonoids in xanthoceras sorbifolia |
| topic | Xanthoceras sorbifolia Flavonoids Whole genome resequencing SNP screening GWAS |
| url | https://doi.org/10.1038/s41598-025-00514-4 |
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