Health Status and Disinfection Prior to Grafting Affect the Phenolic Profile of Grapevine Hetero-Grafts and Grafting Yield
Grapevine trunk disease (GTD) is a major threat to grapevine propagation, severely affecting the growth and development of young vines. As one of the most destructive plant diseases in the world, GTD spreads easily through propagation material and threatens the sustainability of vineyards. While eff...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/3/444 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849717643862343680 |
|---|---|
| author | Saša Krošelj Maja Mikulic-Petkovsek Domen Kjuder Anja Pavlin Matevž Likar Andreja Škvarč Katerina Biniari Denis Rusjan |
| author_facet | Saša Krošelj Maja Mikulic-Petkovsek Domen Kjuder Anja Pavlin Matevž Likar Andreja Škvarč Katerina Biniari Denis Rusjan |
| author_sort | Saša Krošelj |
| collection | DOAJ |
| description | Grapevine trunk disease (GTD) is a major threat to grapevine propagation, severely affecting the growth and development of young vines. As one of the most destructive plant diseases in the world, GTD spreads easily through propagation material and threatens the sustainability of vineyards. While effective, biologically friendly treatments remain unavailable. This study investigated the graft yield, the growth potential of grapevine hetero-grafts, and phenolic responses focusing on (i) GTD scion health status (healthy—HLT; asymptomatic—ASYM; symptomatic—SYM) and (ii) disinfection methods. Grafting with HLT scions achieved the highest yield rates, particularly with Serenade<sup>®</sup> ASO (75%) and BioAction ES (79%), while infected scions showed lower yields. The growth potential of the scions was not affected by the disinfection method or the health status of the scions. Phenolic composition varied between scions, graft callus, rootstock canes, and roots, with scion health status strongly influencing most metabolites. Higher levels of flavanols were observed in HLT scions treated with BioAction ES and Serenade<sup>®</sup> ASO, with these treatments resulting in 1.6 and 1.5 times higher procyanidin dimer levels, respectively, compared to Beltanol. Flavanols and stilbenes were lower in the callus tissue of grafts with healthy scions compared to infected scions. Rootstock also showed higher levels of catechin and procyanidin dimers in grafts with HLT scions. These results indicate that the health status of scion GTD and the disinfection methods significantly influence the graft yield and phenolic composition, providing valuable insights for GTD management. |
| format | Article |
| id | doaj-art-b694b6dd2fa54ea1b312b30b0a43dcf5 |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-b694b6dd2fa54ea1b312b30b0a43dcf52025-08-20T03:12:35ZengMDPI AGPlants2223-77472025-02-0114344410.3390/plants14030444Health Status and Disinfection Prior to Grafting Affect the Phenolic Profile of Grapevine Hetero-Grafts and Grafting YieldSaša Krošelj0Maja Mikulic-Petkovsek1Domen Kjuder2Anja Pavlin3Matevž Likar4Andreja Škvarč5Katerina Biniari6Denis Rusjan7Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaDepartment of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaDepartment of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaDepartment of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaDepartment of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, SI-1000 Ljubljana, SloveniaChamber of Agriculture and Forestry of Slovenia, Agriculture and Forestry Institute Nova Gorica, SI-5101 Nova Gorica, SloveniaLaboratory of Viticulture, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceDepartment of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, SloveniaGrapevine trunk disease (GTD) is a major threat to grapevine propagation, severely affecting the growth and development of young vines. As one of the most destructive plant diseases in the world, GTD spreads easily through propagation material and threatens the sustainability of vineyards. While effective, biologically friendly treatments remain unavailable. This study investigated the graft yield, the growth potential of grapevine hetero-grafts, and phenolic responses focusing on (i) GTD scion health status (healthy—HLT; asymptomatic—ASYM; symptomatic—SYM) and (ii) disinfection methods. Grafting with HLT scions achieved the highest yield rates, particularly with Serenade<sup>®</sup> ASO (75%) and BioAction ES (79%), while infected scions showed lower yields. The growth potential of the scions was not affected by the disinfection method or the health status of the scions. Phenolic composition varied between scions, graft callus, rootstock canes, and roots, with scion health status strongly influencing most metabolites. Higher levels of flavanols were observed in HLT scions treated with BioAction ES and Serenade<sup>®</sup> ASO, with these treatments resulting in 1.6 and 1.5 times higher procyanidin dimer levels, respectively, compared to Beltanol. Flavanols and stilbenes were lower in the callus tissue of grafts with healthy scions compared to infected scions. Rootstock also showed higher levels of catechin and procyanidin dimers in grafts with HLT scions. These results indicate that the health status of scion GTD and the disinfection methods significantly influence the graft yield and phenolic composition, providing valuable insights for GTD management.https://www.mdpi.com/2223-7747/14/3/444grapevine trunk diseasesEscanurserysecondary metabolitesflavanolsstilbenes |
| spellingShingle | Saša Krošelj Maja Mikulic-Petkovsek Domen Kjuder Anja Pavlin Matevž Likar Andreja Škvarč Katerina Biniari Denis Rusjan Health Status and Disinfection Prior to Grafting Affect the Phenolic Profile of Grapevine Hetero-Grafts and Grafting Yield Plants grapevine trunk diseases Esca nursery secondary metabolites flavanols stilbenes |
| title | Health Status and Disinfection Prior to Grafting Affect the Phenolic Profile of Grapevine Hetero-Grafts and Grafting Yield |
| title_full | Health Status and Disinfection Prior to Grafting Affect the Phenolic Profile of Grapevine Hetero-Grafts and Grafting Yield |
| title_fullStr | Health Status and Disinfection Prior to Grafting Affect the Phenolic Profile of Grapevine Hetero-Grafts and Grafting Yield |
| title_full_unstemmed | Health Status and Disinfection Prior to Grafting Affect the Phenolic Profile of Grapevine Hetero-Grafts and Grafting Yield |
| title_short | Health Status and Disinfection Prior to Grafting Affect the Phenolic Profile of Grapevine Hetero-Grafts and Grafting Yield |
| title_sort | health status and disinfection prior to grafting affect the phenolic profile of grapevine hetero grafts and grafting yield |
| topic | grapevine trunk diseases Esca nursery secondary metabolites flavanols stilbenes |
| url | https://www.mdpi.com/2223-7747/14/3/444 |
| work_keys_str_mv | AT sasakroselj healthstatusanddisinfectionpriortograftingaffectthephenolicprofileofgrapevineheterograftsandgraftingyield AT majamikulicpetkovsek healthstatusanddisinfectionpriortograftingaffectthephenolicprofileofgrapevineheterograftsandgraftingyield AT domenkjuder healthstatusanddisinfectionpriortograftingaffectthephenolicprofileofgrapevineheterograftsandgraftingyield AT anjapavlin healthstatusanddisinfectionpriortograftingaffectthephenolicprofileofgrapevineheterograftsandgraftingyield AT matevzlikar healthstatusanddisinfectionpriortograftingaffectthephenolicprofileofgrapevineheterograftsandgraftingyield AT andrejaskvarc healthstatusanddisinfectionpriortograftingaffectthephenolicprofileofgrapevineheterograftsandgraftingyield AT katerinabiniari healthstatusanddisinfectionpriortograftingaffectthephenolicprofileofgrapevineheterograftsandgraftingyield AT denisrusjan healthstatusanddisinfectionpriortograftingaffectthephenolicprofileofgrapevineheterograftsandgraftingyield |