One-step Cre-loxP organism creation by TAx9

One-step Cre-loxP organism creation by TAx9

Abstract The creation of organisms with Cre-loxP conditional gene recombination systems often faces challenges, particularly when creating the initial (F0) generation with both a Cre recombinase and a DNA site flanked by loxP elements (floxed site). The primary reason is that it is difficult to synt...

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Bibliographic Details
Main Authors: Martin Miguel Casco-Robles, Tomoki Echigoya, Takeaki Shimazaki, Yuri Murakami, Masaya Hirano, Fumiaki Maruo, Seiya Mizuno, Satoru Takahashi, Chikafumi Chiba
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-07759-9
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