Bta-Let-7d Modulation of Oxidative Stress Induced by Potassium Permanganate in Bovine Endometrial Cells via IGF1R/PI3K/AKT Signaling Pathway
Oxidative stress is a significant factor affecting reproductive efficiency in dairy cows, contributing to conditions such as endometritis that impair fertility and milk production. This study investigates the molecular mechanisms by which bta-let-7d modulates the oxidative stress responses induced b...
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MDPI AG
2025-04-01
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| Series: | Antioxidants |
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| author | Wenjing Liu Talha Umar Wen Feng Bohan Zhang Jinxin Zhang Han Zhou Nuoer Chen Ganzhen Deng Siyu Xiao |
| author_facet | Wenjing Liu Talha Umar Wen Feng Bohan Zhang Jinxin Zhang Han Zhou Nuoer Chen Ganzhen Deng Siyu Xiao |
| author_sort | Wenjing Liu |
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| description | Oxidative stress is a significant factor affecting reproductive efficiency in dairy cows, contributing to conditions such as endometritis that impair fertility and milk production. This study investigates the molecular mechanisms by which bta-let-7d modulates the oxidative stress responses induced by potassium permanganate (KMnO<sub>4</sub>) in bovine endometrial epithelial cells (BEECs). Using KMnO<sub>4</sub> to induce oxidative stress, we observed significant increases in reactive oxygen species (ROS) and malondialdehyde (MDA) levels, accompanied by decreased activities of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD). Quantitative PCR and Western blot analyses indicated a negative correlation between IGF1R and bta-let-7d expression in oxidative-stress-affected tissues, suggesting opposing roles in managing stress responses. Following KMnO<sub>4</sub> treatment, there was marked downregulation of anti-apoptotic genes and an upregulation of pro-apoptotic markers, alongside diminished antioxidant capacity. Mechanistically, bta-let-7d targets IGF1R, leading to the suppression of the PI3K/AKT signaling pathway and exacerbating oxidative damage. In vivo experiments further confirmed the impact of KMnO<sub>4</sub> exposure on IGF1R expression. These findings provide novel insights into the mechanisms by which KMnO<sub>4</sub> induces oxidative stress and apoptosis in bovine uterus. They highlight the potential for therapeutic strategies targeting the bta-let-7d/IGF1R axis to enhance reproductive health management in dairy cows, offering a promising avenue for mitigating oxidative-stress-related reproductive disorders. |
| format | Article |
| id | doaj-art-b690e4a7654b42ee810ac5874ecdde65 |
| institution | OA Journals |
| issn | 2076-3921 |
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| publishDate | 2025-04-01 |
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| series | Antioxidants |
| spelling | doaj-art-b690e4a7654b42ee810ac5874ecdde652025-08-20T02:24:43ZengMDPI AGAntioxidants2076-39212025-04-0114444410.3390/antiox14040444Bta-Let-7d Modulation of Oxidative Stress Induced by Potassium Permanganate in Bovine Endometrial Cells via IGF1R/PI3K/AKT Signaling PathwayWenjing Liu0Talha Umar1Wen Feng2Bohan Zhang3Jinxin Zhang4Han Zhou5Nuoer Chen6Ganzhen Deng7Siyu Xiao8Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaOxidative stress is a significant factor affecting reproductive efficiency in dairy cows, contributing to conditions such as endometritis that impair fertility and milk production. This study investigates the molecular mechanisms by which bta-let-7d modulates the oxidative stress responses induced by potassium permanganate (KMnO<sub>4</sub>) in bovine endometrial epithelial cells (BEECs). Using KMnO<sub>4</sub> to induce oxidative stress, we observed significant increases in reactive oxygen species (ROS) and malondialdehyde (MDA) levels, accompanied by decreased activities of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD). Quantitative PCR and Western blot analyses indicated a negative correlation between IGF1R and bta-let-7d expression in oxidative-stress-affected tissues, suggesting opposing roles in managing stress responses. Following KMnO<sub>4</sub> treatment, there was marked downregulation of anti-apoptotic genes and an upregulation of pro-apoptotic markers, alongside diminished antioxidant capacity. Mechanistically, bta-let-7d targets IGF1R, leading to the suppression of the PI3K/AKT signaling pathway and exacerbating oxidative damage. In vivo experiments further confirmed the impact of KMnO<sub>4</sub> exposure on IGF1R expression. These findings provide novel insights into the mechanisms by which KMnO<sub>4</sub> induces oxidative stress and apoptosis in bovine uterus. They highlight the potential for therapeutic strategies targeting the bta-let-7d/IGF1R axis to enhance reproductive health management in dairy cows, offering a promising avenue for mitigating oxidative-stress-related reproductive disorders.https://www.mdpi.com/2076-3921/14/4/444potassium permanganatebta-let-7dIGF1Roxidative stressdairy cows |
| spellingShingle | Wenjing Liu Talha Umar Wen Feng Bohan Zhang Jinxin Zhang Han Zhou Nuoer Chen Ganzhen Deng Siyu Xiao Bta-Let-7d Modulation of Oxidative Stress Induced by Potassium Permanganate in Bovine Endometrial Cells via IGF1R/PI3K/AKT Signaling Pathway Antioxidants potassium permanganate bta-let-7d IGF1R oxidative stress dairy cows |
| title | Bta-Let-7d Modulation of Oxidative Stress Induced by Potassium Permanganate in Bovine Endometrial Cells via IGF1R/PI3K/AKT Signaling Pathway |
| title_full | Bta-Let-7d Modulation of Oxidative Stress Induced by Potassium Permanganate in Bovine Endometrial Cells via IGF1R/PI3K/AKT Signaling Pathway |
| title_fullStr | Bta-Let-7d Modulation of Oxidative Stress Induced by Potassium Permanganate in Bovine Endometrial Cells via IGF1R/PI3K/AKT Signaling Pathway |
| title_full_unstemmed | Bta-Let-7d Modulation of Oxidative Stress Induced by Potassium Permanganate in Bovine Endometrial Cells via IGF1R/PI3K/AKT Signaling Pathway |
| title_short | Bta-Let-7d Modulation of Oxidative Stress Induced by Potassium Permanganate in Bovine Endometrial Cells via IGF1R/PI3K/AKT Signaling Pathway |
| title_sort | bta let 7d modulation of oxidative stress induced by potassium permanganate in bovine endometrial cells via igf1r pi3k akt signaling pathway |
| topic | potassium permanganate bta-let-7d IGF1R oxidative stress dairy cows |
| url | https://www.mdpi.com/2076-3921/14/4/444 |
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