Packaged release and targeted delivery of cytokines by migrasomes in circulation
Abstract In dynamic systems like the circulatory system, establishing localized cytokine gradients is challenging. Upon lipopolysaccharide (LPS) stimulation, we observed that monocytes release numerous migrasomes enriched with inflammatory cytokines, such as TNF-α and IL-6. These cytokines are trans...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2024-12-01
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| Series: | Cell Discovery |
| Online Access: | https://doi.org/10.1038/s41421-024-00749-x |
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| _version_ | 1846121875607912448 |
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| author | Haifeng Jiao Xiaopeng Li Ying Li Ziyi Guo Yuzhuo Yang Yiqun Luo Xiaoyu Hu Li Yu |
| author_facet | Haifeng Jiao Xiaopeng Li Ying Li Ziyi Guo Yuzhuo Yang Yiqun Luo Xiaoyu Hu Li Yu |
| author_sort | Haifeng Jiao |
| collection | DOAJ |
| description | Abstract In dynamic systems like the circulatory system, establishing localized cytokine gradients is challenging. Upon lipopolysaccharide (LPS) stimulation, we observed that monocytes release numerous migrasomes enriched with inflammatory cytokines, such as TNF-α and IL-6. These cytokines are transported into migrasomes via secretory carriers, leading to their immediate exocytosis or eventual release from detached migrasomes. We successfully isolated TNF-α and IL-6-enriched, monocyte-derived migrasomes from the blood of LPS-treated mice. Total secretion analysis revealed a substantial amount of TNF-α and IL-6 released in a migrasome-packaged form. Thus, detached, monocyte-derived migrasomes represent a type of extracellular vesicle highly enriched with cytokines. Physiologically, these cytokine-laden migrasomes rapidly accumulate at local sites of inflammation, effectively creating a concentrated source of cytokines. Our research uncovers novel mechanisms for cytokine release and delivery, providing new insights into immune response modulation. |
| format | Article |
| id | doaj-art-b68f94aec8f24a05b4fa8e712db5de32 |
| institution | Kabale University |
| issn | 2056-5968 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Discovery |
| spelling | doaj-art-b68f94aec8f24a05b4fa8e712db5de322024-12-15T12:06:10ZengNature Publishing GroupCell Discovery2056-59682024-12-0110111210.1038/s41421-024-00749-xPackaged release and targeted delivery of cytokines by migrasomes in circulationHaifeng Jiao0Xiaopeng Li1Ying Li2Ziyi Guo3Yuzhuo Yang4Yiqun Luo5Xiaoyu Hu6Li Yu7State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Centre for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua UniversityState Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Centre for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua UniversityState Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Centre for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua UniversityInstitute for Immunology, Tsinghua University-Peking University Joint Centre for Life Sciences, School of Medicine, Tsinghua UniversityInstitute for Immunology, Tsinghua University-Peking University Joint Centre for Life Sciences, School of Medicine, Tsinghua UniversityState Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Centre for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua UniversityInstitute for Immunology, Tsinghua University-Peking University Joint Centre for Life Sciences, School of Medicine, Tsinghua UniversityState Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Centre for Life Sciences, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua UniversityAbstract In dynamic systems like the circulatory system, establishing localized cytokine gradients is challenging. Upon lipopolysaccharide (LPS) stimulation, we observed that monocytes release numerous migrasomes enriched with inflammatory cytokines, such as TNF-α and IL-6. These cytokines are transported into migrasomes via secretory carriers, leading to their immediate exocytosis or eventual release from detached migrasomes. We successfully isolated TNF-α and IL-6-enriched, monocyte-derived migrasomes from the blood of LPS-treated mice. Total secretion analysis revealed a substantial amount of TNF-α and IL-6 released in a migrasome-packaged form. Thus, detached, monocyte-derived migrasomes represent a type of extracellular vesicle highly enriched with cytokines. Physiologically, these cytokine-laden migrasomes rapidly accumulate at local sites of inflammation, effectively creating a concentrated source of cytokines. Our research uncovers novel mechanisms for cytokine release and delivery, providing new insights into immune response modulation.https://doi.org/10.1038/s41421-024-00749-x |
| spellingShingle | Haifeng Jiao Xiaopeng Li Ying Li Ziyi Guo Yuzhuo Yang Yiqun Luo Xiaoyu Hu Li Yu Packaged release and targeted delivery of cytokines by migrasomes in circulation Cell Discovery |
| title | Packaged release and targeted delivery of cytokines by migrasomes in circulation |
| title_full | Packaged release and targeted delivery of cytokines by migrasomes in circulation |
| title_fullStr | Packaged release and targeted delivery of cytokines by migrasomes in circulation |
| title_full_unstemmed | Packaged release and targeted delivery of cytokines by migrasomes in circulation |
| title_short | Packaged release and targeted delivery of cytokines by migrasomes in circulation |
| title_sort | packaged release and targeted delivery of cytokines by migrasomes in circulation |
| url | https://doi.org/10.1038/s41421-024-00749-x |
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