Identification of ERBB Pathway-Activated Cells in Triple-Negative Breast Cancer
Intratumor heterogeneity within a single tumor mass is one of the hallmarks of malignancy and has been reported in various tumor types. The molecular characterization of intratumor heterogeneity in breast cancer is a significant challenge for effective treatment. Using single-cell RNA sequencing (RN...
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| Language: | English |
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BioMed Central
2019-03-01
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| Series: | Genomics & Informatics |
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| Online Access: | http://genominfo.org/upload/pdf/gi-2019-17-1-e3.pdf |
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| _version_ | 1832570982816022528 |
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| author | Soo Young Cho |
| author_facet | Soo Young Cho |
| author_sort | Soo Young Cho |
| collection | DOAJ |
| description | Intratumor heterogeneity within a single tumor mass is one of the hallmarks of malignancy and has been reported in various tumor types. The molecular characterization of intratumor heterogeneity in breast cancer is a significant challenge for effective treatment. Using single-cell RNA sequencing (RNA-seq) data from a public resource, an ERBB pathway activated triple-negative cell population was identified. The differential expression of three subtyping marker genes (ERBB2, ESR1, and PGR) was not changed in the bulk RNA-seq data, but the single-cell transcriptomes showed intratumor heterogeneity. This result shows that ERBB signaling is activated using an indirect route and that the molecular subtype is changed on a single-cell level. Our data propose a different view on breast cancer subtypes, clarifying much confusion in this field and contributing to precision medicine. |
| format | Article |
| id | doaj-art-b68a0d0fd3894c39b135928aa1dd89b2 |
| institution | Kabale University |
| issn | 2234-0742 |
| language | English |
| publishDate | 2019-03-01 |
| publisher | BioMed Central |
| record_format | Article |
| series | Genomics & Informatics |
| spelling | doaj-art-b68a0d0fd3894c39b135928aa1dd89b22025-02-02T13:28:54ZengBioMed CentralGenomics & Informatics2234-07422019-03-0117110.5808/GI.2019.17.1.e3546Identification of ERBB Pathway-Activated Cells in Triple-Negative Breast CancerSoo Young ChoIntratumor heterogeneity within a single tumor mass is one of the hallmarks of malignancy and has been reported in various tumor types. The molecular characterization of intratumor heterogeneity in breast cancer is a significant challenge for effective treatment. Using single-cell RNA sequencing (RNA-seq) data from a public resource, an ERBB pathway activated triple-negative cell population was identified. The differential expression of three subtyping marker genes (ERBB2, ESR1, and PGR) was not changed in the bulk RNA-seq data, but the single-cell transcriptomes showed intratumor heterogeneity. This result shows that ERBB signaling is activated using an indirect route and that the molecular subtype is changed on a single-cell level. Our data propose a different view on breast cancer subtypes, clarifying much confusion in this field and contributing to precision medicine.http://genominfo.org/upload/pdf/gi-2019-17-1-e3.pdfbreast neoplasmsERBB signaling pathwayintratumor heterogeneitymolecular subtypingsingle-cell RNA-seq |
| spellingShingle | Soo Young Cho Identification of ERBB Pathway-Activated Cells in Triple-Negative Breast Cancer Genomics & Informatics breast neoplasms ERBB signaling pathway intratumor heterogeneity molecular subtyping single-cell RNA-seq |
| title | Identification of ERBB Pathway-Activated Cells in Triple-Negative Breast Cancer |
| title_full | Identification of ERBB Pathway-Activated Cells in Triple-Negative Breast Cancer |
| title_fullStr | Identification of ERBB Pathway-Activated Cells in Triple-Negative Breast Cancer |
| title_full_unstemmed | Identification of ERBB Pathway-Activated Cells in Triple-Negative Breast Cancer |
| title_short | Identification of ERBB Pathway-Activated Cells in Triple-Negative Breast Cancer |
| title_sort | identification of erbb pathway activated cells in triple negative breast cancer |
| topic | breast neoplasms ERBB signaling pathway intratumor heterogeneity molecular subtyping single-cell RNA-seq |
| url | http://genominfo.org/upload/pdf/gi-2019-17-1-e3.pdf |
| work_keys_str_mv | AT sooyoungcho identificationoferbbpathwayactivatedcellsintriplenegativebreastcancer |