The effect of priming with gibberellic acid on improving the germination of seeds obtained from self-pollinated of different varieties of Petunia hybrida

Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in th...

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Main Authors: Leyla Cheheltanan, Ali Tehranifar, Mahmoud Shoor, Seyyed Hossein Neamati, Saeed Khosravi
Format: Article
Language:fas
Published: Seed and Plant Certification and Registration Institute 2025-02-01
Series:علوم و فناوری بذر ایران
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Online Access:https://ijsst.areeo.ac.ir/article_132542_8e2720ff006ece19a6854260292a517e.pdf
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author Leyla Cheheltanan
Ali Tehranifar
Mahmoud Shoor
Seyyed Hossein Neamati
Saeed Khosravi
author_facet Leyla Cheheltanan
Ali Tehranifar
Mahmoud Shoor
Seyyed Hossein Neamati
Saeed Khosravi
author_sort Leyla Cheheltanan
collection DOAJ
description Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in the improves of rapid and continuous seed germination and subsequent plant growth. Therefore, this research aimed to investigate the effect of different concentrations of gibberellic acid on the improvement of seed germination resulting from self-pollination in various varieties of Petunia hybrida in a factorial experiment with three replications as a completely randomized design. The treatments consisted of four levels of gibberellic acid (0, 50, 100, and 150 mg/L) and seeds obtained from self-pollination of Iranian Petunia, Tango Blue, Tritunia Pink Morn, and Tritunia White. The results indicated that seed priming with gibberellic acid, especially at a concentration of 100 mg/L, led to a significant increase in the activity of α-amylase and β-amylase by 6.56% and 1.57%, respectively. This increase resulted in 8.54% rise in soluble sugars content, providing energy that significantly enhanced germination percentage, germination speed, average time required for germination, seed vigor index, germination energy, and the fresh and dry weight of plumule and radicle. Moreover, among different varieties, seeds obtained from self-pollination of Iranian petunia demonstrated a higher germination percentage and germination speed compared to seeds from other varieties, indicating a superior capability in maintaining vigor potential, germination percentage, and germination speed.
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institution Kabale University
issn 2322-2646
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publisher Seed and Plant Certification and Registration Institute
record_format Article
series علوم و فناوری بذر ایران
spelling doaj-art-b67f78b027e34f80bc3dc891b741429a2025-08-20T03:30:49ZfasSeed and Plant Certification and Registration Instituteعلوم و فناوری بذر ایران2322-26462588-46382025-02-0114111610.22092/ijsst.2024.364475.1510132542The effect of priming with gibberellic acid on improving the germination of seeds obtained from self-pollinated of different varieties of Petunia hybridaLeyla Cheheltanan0Ali Tehranifar1Mahmoud Shoor2Seyyed Hossein Neamati3Saeed Khosravi4Ph.D., Department of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.Professor, Department of Horticultural Science and landscape, Faculty of Agriculture, Ferdowsi University of Mashhad,, Mashhad, Iran.Associate Professor, Department of Horticultural Science and Landscape, Faculty of Agriculture,, Ferdowsi University of Mashhad, Mashhad, Iran.Assistant Professor, Department of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.Ph.D. Student, Department of Horticultural Science and Landscape, Ferdowsi University of Mashhad, Mashhad, Iran.Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in the improves of rapid and continuous seed germination and subsequent plant growth. Therefore, this research aimed to investigate the effect of different concentrations of gibberellic acid on the improvement of seed germination resulting from self-pollination in various varieties of Petunia hybrida in a factorial experiment with three replications as a completely randomized design. The treatments consisted of four levels of gibberellic acid (0, 50, 100, and 150 mg/L) and seeds obtained from self-pollination of Iranian Petunia, Tango Blue, Tritunia Pink Morn, and Tritunia White. The results indicated that seed priming with gibberellic acid, especially at a concentration of 100 mg/L, led to a significant increase in the activity of α-amylase and β-amylase by 6.56% and 1.57%, respectively. This increase resulted in 8.54% rise in soluble sugars content, providing energy that significantly enhanced germination percentage, germination speed, average time required for germination, seed vigor index, germination energy, and the fresh and dry weight of plumule and radicle. Moreover, among different varieties, seeds obtained from self-pollination of Iranian petunia demonstrated a higher germination percentage and germination speed compared to seeds from other varieties, indicating a superior capability in maintaining vigor potential, germination percentage, and germination speed.https://ijsst.areeo.ac.ir/article_132542_8e2720ff006ece19a6854260292a517e.pdfα-amylaseβ-amylasegermination percentagesoluble sugars content
spellingShingle Leyla Cheheltanan
Ali Tehranifar
Mahmoud Shoor
Seyyed Hossein Neamati
Saeed Khosravi
The effect of priming with gibberellic acid on improving the germination of seeds obtained from self-pollinated of different varieties of Petunia hybrida
علوم و فناوری بذر ایران
α-amylase
β-amylase
germination percentage
soluble sugars content
title The effect of priming with gibberellic acid on improving the germination of seeds obtained from self-pollinated of different varieties of Petunia hybrida
title_full The effect of priming with gibberellic acid on improving the germination of seeds obtained from self-pollinated of different varieties of Petunia hybrida
title_fullStr The effect of priming with gibberellic acid on improving the germination of seeds obtained from self-pollinated of different varieties of Petunia hybrida
title_full_unstemmed The effect of priming with gibberellic acid on improving the germination of seeds obtained from self-pollinated of different varieties of Petunia hybrida
title_short The effect of priming with gibberellic acid on improving the germination of seeds obtained from self-pollinated of different varieties of Petunia hybrida
title_sort effect of priming with gibberellic acid on improving the germination of seeds obtained from self pollinated of different varieties of petunia hybrida
topic α-amylase
β-amylase
germination percentage
soluble sugars content
url https://ijsst.areeo.ac.ir/article_132542_8e2720ff006ece19a6854260292a517e.pdf
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