Prevalence of Mycobacterium tuberculosis in Patients Suffering from Chronic Respiratory Diseases in a Tertiary Care Hospital Using Gene Xpert as the Diagnostic Tool

Background: Chronic respiratory diseases constitute a grave problem throughout the world and particularly in the middle- and low-income countries. The burden of these diseases leads to poor quality of life and disability of affected individuals leading to premature deaths and a great economic loss t...

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Main Authors: Baishali Das, Dina Raja
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2022-01-01
Series:Assam Journal of Internal Medicine
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Online Access:https://journals.lww.com/10.4103/ajoim.ajoim_3_21
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author Baishali Das
Dina Raja
author_facet Baishali Das
Dina Raja
author_sort Baishali Das
collection DOAJ
description Background: Chronic respiratory diseases constitute a grave problem throughout the world and particularly in the middle- and low-income countries. The burden of these diseases leads to poor quality of life and disability of affected individuals leading to premature deaths and a great economic loss to their families and society. Tuberculosis remains one of the deadliest communicable diseases. Though culture is the gold standard for the diagnosis of tuberculosis, the long period of time required for a positive result leads to the transmission of the disease in the community. Objective: The study was conducted to study the prevalence of Mycobacterium tuberculosis in patients suffering from chronic respiratory diseases. Materials and Methods: Bronchoalveolar lavage fluid samples of patients with chronic respiratory diseases undergoing bronchoscopy were collected under aseptic conditions after obtaining approval from the Institutional Ethics Committee. Each sample was subjected to Ziehl Neelsen stain and Cartridge Based Nucleic Acid Amplification Test (CBNAAT) on Xpert MTB/RIF manufactured by Cepheid (Sunnyvale, CA). Results: Eighteen of 110 cases (16.36%) showed the presence of M. tuberculosis in the samples, of which rifampicin resistance was detected in 2 (11.11%) cases. Conclusions: M. tuberculosis remains a common underlying pathogen in the cases of chronic respiratory diseases.
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spelling doaj-art-b67f69362ff748c1b602d31ff550e5122025-08-20T03:16:46ZengWolters Kluwer Medknow PublicationsAssam Journal of Internal Medicine2278-82392022-01-01121101310.4103/ajoim.ajoim_3_21Prevalence of Mycobacterium tuberculosis in Patients Suffering from Chronic Respiratory Diseases in a Tertiary Care Hospital Using Gene Xpert as the Diagnostic ToolBaishali DasDina RajaBackground: Chronic respiratory diseases constitute a grave problem throughout the world and particularly in the middle- and low-income countries. The burden of these diseases leads to poor quality of life and disability of affected individuals leading to premature deaths and a great economic loss to their families and society. Tuberculosis remains one of the deadliest communicable diseases. Though culture is the gold standard for the diagnosis of tuberculosis, the long period of time required for a positive result leads to the transmission of the disease in the community. Objective: The study was conducted to study the prevalence of Mycobacterium tuberculosis in patients suffering from chronic respiratory diseases. Materials and Methods: Bronchoalveolar lavage fluid samples of patients with chronic respiratory diseases undergoing bronchoscopy were collected under aseptic conditions after obtaining approval from the Institutional Ethics Committee. Each sample was subjected to Ziehl Neelsen stain and Cartridge Based Nucleic Acid Amplification Test (CBNAAT) on Xpert MTB/RIF manufactured by Cepheid (Sunnyvale, CA). Results: Eighteen of 110 cases (16.36%) showed the presence of M. tuberculosis in the samples, of which rifampicin resistance was detected in 2 (11.11%) cases. Conclusions: M. tuberculosis remains a common underlying pathogen in the cases of chronic respiratory diseases.https://journals.lww.com/10.4103/ajoim.ajoim_3_21chronic respiratory diseasesgene xpertmycobacterium tuberculosis
spellingShingle Baishali Das
Dina Raja
Prevalence of Mycobacterium tuberculosis in Patients Suffering from Chronic Respiratory Diseases in a Tertiary Care Hospital Using Gene Xpert as the Diagnostic Tool
Assam Journal of Internal Medicine
chronic respiratory diseases
gene xpert
mycobacterium tuberculosis
title Prevalence of Mycobacterium tuberculosis in Patients Suffering from Chronic Respiratory Diseases in a Tertiary Care Hospital Using Gene Xpert as the Diagnostic Tool
title_full Prevalence of Mycobacterium tuberculosis in Patients Suffering from Chronic Respiratory Diseases in a Tertiary Care Hospital Using Gene Xpert as the Diagnostic Tool
title_fullStr Prevalence of Mycobacterium tuberculosis in Patients Suffering from Chronic Respiratory Diseases in a Tertiary Care Hospital Using Gene Xpert as the Diagnostic Tool
title_full_unstemmed Prevalence of Mycobacterium tuberculosis in Patients Suffering from Chronic Respiratory Diseases in a Tertiary Care Hospital Using Gene Xpert as the Diagnostic Tool
title_short Prevalence of Mycobacterium tuberculosis in Patients Suffering from Chronic Respiratory Diseases in a Tertiary Care Hospital Using Gene Xpert as the Diagnostic Tool
title_sort prevalence of mycobacterium tuberculosis in patients suffering from chronic respiratory diseases in a tertiary care hospital using gene xpert as the diagnostic tool
topic chronic respiratory diseases
gene xpert
mycobacterium tuberculosis
url https://journals.lww.com/10.4103/ajoim.ajoim_3_21
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AT dinaraja prevalenceofmycobacteriumtuberculosisinpatientssufferingfromchronicrespiratorydiseasesinatertiarycarehospitalusinggenexpertasthediagnostictool