Research on Genotoxicity Evaluation of the Fungal Alpha-Amylase Enzyme on <i>Drosophila melanogaster</i>
Alpha-amylase is an extracellular enzyme abundantly produced from fungal sources. The catalytic activity of microbial enzymes is higher, more stable, and economical compared to plant and animal enzymes; they can be produced in large quantities in a short time and do not produce unwanted by-products....
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2025-02-01
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| author | Arzu Taşpınar Ünal Fahriye Zemheri Navruz Safiye Elif Korcan Sinan İnce Emine Uygur Göçer |
| author_facet | Arzu Taşpınar Ünal Fahriye Zemheri Navruz Safiye Elif Korcan Sinan İnce Emine Uygur Göçer |
| author_sort | Arzu Taşpınar Ünal |
| collection | DOAJ |
| description | Alpha-amylase is an extracellular enzyme abundantly produced from fungal sources. The catalytic activity of microbial enzymes is higher, more stable, and economical compared to plant and animal enzymes; they can be produced in large quantities in a short time and do not produce unwanted by-products. In this study, the genotoxic effect of different concentrations (25 mg/mL, 50 mg/mL, and 100 mg/mL) of a native fungal thermostable alpha-amylase enzyme, produced from the <i>Aspergillus niger</i> G2-1 isolate with an enzyme activity of 38.6 U/mg, was investigated on the <i>Drosophila melanogaster</i> model organism. The effect of the alpha-amylase enzyme added to the culture medium on the developmental performance of <i>D. melanogaster</i> was assessed through larval toxicity analysis, its effect on DNA damage through the comet assay, and its response to oxidative stress through various biochemical parameters. As a result, it was determined that low-dose alpha-amylase enzyme concentration (25 mg/mL) did not cause intracellular oxidative stress, did not cause genotoxicity, and did not adversely affect growth performance, although feeding with alpha-amylase at 50 mg/mL and 100 mg/mL concentrations caused a significant decrease in the survival rate of <i>D. melanogaster</i> larvae and an increase in DNA damage rate in imagos. However, oxidative stress parameters in adult <i>D. melanogaster</i> did not change after the same alpha-amylase application. |
| format | Article |
| id | doaj-art-b67c7ccd14184bfbbe9f36c793062220 |
| institution | OA Journals |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-b67c7ccd14184bfbbe9f36c7930622202025-08-20T02:11:18ZengMDPI AGBiology2079-77372025-02-0114321910.3390/biology14030219Research on Genotoxicity Evaluation of the Fungal Alpha-Amylase Enzyme on <i>Drosophila melanogaster</i>Arzu Taşpınar Ünal0Fahriye Zemheri Navruz1Safiye Elif Korcan2Sinan İnce3Emine Uygur Göçer4Department of Agricultural Biotechnology, Faculty of Agriculture, Iğdır University, Iğdır 76000, TürkiyeDepartment of Molecular Biology and Genetics, Faculty of Science, Bartın University, Bartın 74110, TürkiyeVocational School of Health Services, Uşak University, Uşak 64100, TürkiyeDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, TürkiyeDepartment of Agricultural Biotechnology, Faculty of Agriculture, Iğdır University, Iğdır 76000, TürkiyeAlpha-amylase is an extracellular enzyme abundantly produced from fungal sources. The catalytic activity of microbial enzymes is higher, more stable, and economical compared to plant and animal enzymes; they can be produced in large quantities in a short time and do not produce unwanted by-products. In this study, the genotoxic effect of different concentrations (25 mg/mL, 50 mg/mL, and 100 mg/mL) of a native fungal thermostable alpha-amylase enzyme, produced from the <i>Aspergillus niger</i> G2-1 isolate with an enzyme activity of 38.6 U/mg, was investigated on the <i>Drosophila melanogaster</i> model organism. The effect of the alpha-amylase enzyme added to the culture medium on the developmental performance of <i>D. melanogaster</i> was assessed through larval toxicity analysis, its effect on DNA damage through the comet assay, and its response to oxidative stress through various biochemical parameters. As a result, it was determined that low-dose alpha-amylase enzyme concentration (25 mg/mL) did not cause intracellular oxidative stress, did not cause genotoxicity, and did not adversely affect growth performance, although feeding with alpha-amylase at 50 mg/mL and 100 mg/mL concentrations caused a significant decrease in the survival rate of <i>D. melanogaster</i> larvae and an increase in DNA damage rate in imagos. However, oxidative stress parameters in adult <i>D. melanogaster</i> did not change after the same alpha-amylase application.https://www.mdpi.com/2079-7737/14/3/219thermostable alpha-amylase<i>Drosophila melanogaster</i>genotoxicity |
| spellingShingle | Arzu Taşpınar Ünal Fahriye Zemheri Navruz Safiye Elif Korcan Sinan İnce Emine Uygur Göçer Research on Genotoxicity Evaluation of the Fungal Alpha-Amylase Enzyme on <i>Drosophila melanogaster</i> Biology thermostable alpha-amylase <i>Drosophila melanogaster</i> genotoxicity |
| title | Research on Genotoxicity Evaluation of the Fungal Alpha-Amylase Enzyme on <i>Drosophila melanogaster</i> |
| title_full | Research on Genotoxicity Evaluation of the Fungal Alpha-Amylase Enzyme on <i>Drosophila melanogaster</i> |
| title_fullStr | Research on Genotoxicity Evaluation of the Fungal Alpha-Amylase Enzyme on <i>Drosophila melanogaster</i> |
| title_full_unstemmed | Research on Genotoxicity Evaluation of the Fungal Alpha-Amylase Enzyme on <i>Drosophila melanogaster</i> |
| title_short | Research on Genotoxicity Evaluation of the Fungal Alpha-Amylase Enzyme on <i>Drosophila melanogaster</i> |
| title_sort | research on genotoxicity evaluation of the fungal alpha amylase enzyme on i drosophila melanogaster i |
| topic | thermostable alpha-amylase <i>Drosophila melanogaster</i> genotoxicity |
| url | https://www.mdpi.com/2079-7737/14/3/219 |
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