Generation of a homozygous DNMT3A knock-out hiPSC line for modeling of cardiovascular diseases associated with clonal hematopoiesis of indeterminate potential
Mutations associated with clonal hematopoiesis of indeterminate potential (CHIP) have been linked to cardiovascular disease (CVD), with DNA methyltransferase 3A (DNMT3A) being the most commonly mutated gene. (Jaiswal et al. 2017; Abplanalp et al. 2021) We generated a genome-edited human induced plur...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506125000571 |
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| author | Christos Triantafyllou Michael Peitz Bernd K. Fleischmann Sarah Rieck |
| author_facet | Christos Triantafyllou Michael Peitz Bernd K. Fleischmann Sarah Rieck |
| author_sort | Christos Triantafyllou |
| collection | DOAJ |
| description | Mutations associated with clonal hematopoiesis of indeterminate potential (CHIP) have been linked to cardiovascular disease (CVD), with DNA methyltransferase 3A (DNMT3A) being the most commonly mutated gene. (Jaiswal et al. 2017; Abplanalp et al. 2021) We generated a genome-edited human induced pluripotent stem cell (hiPSC) line with a homozygous knock-out (KO) of DNMT3A, to mimic loss-of-function mutations, and an isogenic mock-treated control line (mock ctrl). For quality control, we tested the pluripotency of these hiPSC lines and their ability to differentiate into the three germ layers. The generation of these cell lines enables the analysis of cellular pathomechanisms of DNMT3A-related, CHIP-associated CVDs. |
| format | Article |
| id | doaj-art-b666fd051e614d81a53cd05d0ed4d4fe |
| institution | OA Journals |
| issn | 1873-5061 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-b666fd051e614d81a53cd05d0ed4d4fe2025-08-20T02:33:12ZengElsevierStem Cell Research1873-50612025-08-018610370710.1016/j.scr.2025.103707Generation of a homozygous DNMT3A knock-out hiPSC line for modeling of cardiovascular diseases associated with clonal hematopoiesis of indeterminate potentialChristos Triantafyllou0Michael Peitz1Bernd K. Fleischmann2Sarah Rieck3Institute of Physiology I, University of Bonn, Medical Faculty, Bonn, GermanyCell Programming Core Facility, University of Bonn, Medical Faculty, Bonn, GermanyInstitute of Physiology I, University of Bonn, Medical Faculty, Bonn, GermanyInstitute of Physiology I, University of Bonn, Medical Faculty, Bonn, Germany; Corresponding author.Mutations associated with clonal hematopoiesis of indeterminate potential (CHIP) have been linked to cardiovascular disease (CVD), with DNA methyltransferase 3A (DNMT3A) being the most commonly mutated gene. (Jaiswal et al. 2017; Abplanalp et al. 2021) We generated a genome-edited human induced pluripotent stem cell (hiPSC) line with a homozygous knock-out (KO) of DNMT3A, to mimic loss-of-function mutations, and an isogenic mock-treated control line (mock ctrl). For quality control, we tested the pluripotency of these hiPSC lines and their ability to differentiate into the three germ layers. The generation of these cell lines enables the analysis of cellular pathomechanisms of DNMT3A-related, CHIP-associated CVDs.http://www.sciencedirect.com/science/article/pii/S1873506125000571 |
| spellingShingle | Christos Triantafyllou Michael Peitz Bernd K. Fleischmann Sarah Rieck Generation of a homozygous DNMT3A knock-out hiPSC line for modeling of cardiovascular diseases associated with clonal hematopoiesis of indeterminate potential Stem Cell Research |
| title | Generation of a homozygous DNMT3A knock-out hiPSC line for modeling of cardiovascular diseases associated with clonal hematopoiesis of indeterminate potential |
| title_full | Generation of a homozygous DNMT3A knock-out hiPSC line for modeling of cardiovascular diseases associated with clonal hematopoiesis of indeterminate potential |
| title_fullStr | Generation of a homozygous DNMT3A knock-out hiPSC line for modeling of cardiovascular diseases associated with clonal hematopoiesis of indeterminate potential |
| title_full_unstemmed | Generation of a homozygous DNMT3A knock-out hiPSC line for modeling of cardiovascular diseases associated with clonal hematopoiesis of indeterminate potential |
| title_short | Generation of a homozygous DNMT3A knock-out hiPSC line for modeling of cardiovascular diseases associated with clonal hematopoiesis of indeterminate potential |
| title_sort | generation of a homozygous dnmt3a knock out hipsc line for modeling of cardiovascular diseases associated with clonal hematopoiesis of indeterminate potential |
| url | http://www.sciencedirect.com/science/article/pii/S1873506125000571 |
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