Cardioprotective Effect of Peperomia pellucida against Doxorubicin-Induced Cardiotoxicity in Wistar Rats via Modulation of Electrocardiographic and Cardiac Biomarkers

Objectives: This study assessed the electrocardiographic pattern and cardiac inflammatory response of doxorubicin-induced myocardial injury in Wistar rats treated with Peperomia pellucida ethanol extract.Methods: Female Wistar rats (190-200 g) were assigned into five groups of seven rats each. The G...

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Main Authors: Efiok Aniekan Archibong, Justin Atiang Beshel, Idara Asuquo Okon, Glory Aidam Ikum, Stella Chiamaka Anaba, Daniel Udofia Owu
Format: Article
Language:English
Published: Korean Pharmacopuncture Institute 2024-12-01
Series:Journal of Pharmacopuncture
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Online Access:http://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2024.27.4.297
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author Efiok Aniekan Archibong
Justin Atiang Beshel
Idara Asuquo Okon
Glory Aidam Ikum
Stella Chiamaka Anaba
Daniel Udofia Owu
author_facet Efiok Aniekan Archibong
Justin Atiang Beshel
Idara Asuquo Okon
Glory Aidam Ikum
Stella Chiamaka Anaba
Daniel Udofia Owu
author_sort Efiok Aniekan Archibong
collection DOAJ
description Objectives: This study assessed the electrocardiographic pattern and cardiac inflammatory response of doxorubicin-induced myocardial injury in Wistar rats treated with Peperomia pellucida ethanol extract.Methods: Female Wistar rats (190-200 g) were assigned into five groups of seven rats each. The Group 1 (Control group) was given rat chow and drinking water while the Group 2 (doxorubicin group) received intraperitoneal administration of doxorubicin (2 mg/kg) once weekly for three weeks. The Group 3 (Peperomia pellucida group) received 200 mg/ kg of ethanolic extract of Peperomia pellucida daily. Group 4 (Doxorubicin + P. pellucida group) received doxorubicin in addition to Peperomia pellucida. Group 5 (Captopril (50 mg/kg) was administered to another group in addition to P. pellucida while the doxorubicin + captopril group was administered captopril in addition to doxorubicin. Electrical recording and cardiac markers were evaluated.Results: The results revealed a significant (p < 0.01) elevation of T-wave and altered electrocardiographic parameters in the doxorubicin group than the control, P. pellucida, and other experimental groups. The heart rate, cardiac troponin level, lactate dehydrogenase, creatine kinase, angiotensin-converting enzyme activities, and inflammatory biomarkers were significantly (p < 0.01) higher while nitric oxide level was significantly (p < 0.05) reduced in the doxorubicin-only group compared to the control. Cardiac cell hypertrophy and inflammatory cell infiltration were observed due to doxorubicin administration. Treatment with P. pellucida extract and captopril reversed these trends and improved the antioxidants and inflammatory activities.Conclusion: Peperomia pellucida extract improves electrocardiographic pattern, has cardioprotective ability, and prevents doxorubicin-induced myocardial injury probably due to its phytochemical constituents and anti-inflammatory properties.
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spelling doaj-art-b661494150df4cb7a226f066efcf305e2024-12-18T02:47:23ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662024-12-0127429730710.3831/KPI.2024.27.4.297KPI.2024.27.4.297Cardioprotective Effect of Peperomia pellucida against Doxorubicin-Induced Cardiotoxicity in Wistar Rats via Modulation of Electrocardiographic and Cardiac BiomarkersEfiok Aniekan Archibong0Justin Atiang Beshel1Idara Asuquo Okon2Glory Aidam Ikum3Stella Chiamaka Anaba4Daniel Udofia Owu5Department of Physiology, College of Medical Sciences, University of Calabar, Calabar, NigeriaDepartment of Physiology, College of Medical Sciences, University of Calabar, Calabar, NigeriaDepartment of Physiology, Faculty of Biomedical Sciences, Kampala International University, Western Campus, Bushenyi, UgandaDepartment of Physiology, College of Medical Sciences, University of Calabar, Calabar, NigeriaDepartment of Physiology, College of Medical Sciences, University of Calabar, Calabar, NigeriaDepartment of Physiology, Faculty of Biomedical Sciences, Kampala International University, Western Campus, Bushenyi, UgandaObjectives: This study assessed the electrocardiographic pattern and cardiac inflammatory response of doxorubicin-induced myocardial injury in Wistar rats treated with Peperomia pellucida ethanol extract.Methods: Female Wistar rats (190-200 g) were assigned into five groups of seven rats each. The Group 1 (Control group) was given rat chow and drinking water while the Group 2 (doxorubicin group) received intraperitoneal administration of doxorubicin (2 mg/kg) once weekly for three weeks. The Group 3 (Peperomia pellucida group) received 200 mg/ kg of ethanolic extract of Peperomia pellucida daily. Group 4 (Doxorubicin + P. pellucida group) received doxorubicin in addition to Peperomia pellucida. Group 5 (Captopril (50 mg/kg) was administered to another group in addition to P. pellucida while the doxorubicin + captopril group was administered captopril in addition to doxorubicin. Electrical recording and cardiac markers were evaluated.Results: The results revealed a significant (p < 0.01) elevation of T-wave and altered electrocardiographic parameters in the doxorubicin group than the control, P. pellucida, and other experimental groups. The heart rate, cardiac troponin level, lactate dehydrogenase, creatine kinase, angiotensin-converting enzyme activities, and inflammatory biomarkers were significantly (p < 0.01) higher while nitric oxide level was significantly (p < 0.05) reduced in the doxorubicin-only group compared to the control. Cardiac cell hypertrophy and inflammatory cell infiltration were observed due to doxorubicin administration. Treatment with P. pellucida extract and captopril reversed these trends and improved the antioxidants and inflammatory activities.Conclusion: Peperomia pellucida extract improves electrocardiographic pattern, has cardioprotective ability, and prevents doxorubicin-induced myocardial injury probably due to its phytochemical constituents and anti-inflammatory properties.http://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2024.27.4.297captoprilcardiac troponincardiotoxicityelectrocardiographinflammationpeperomia pellucida
spellingShingle Efiok Aniekan Archibong
Justin Atiang Beshel
Idara Asuquo Okon
Glory Aidam Ikum
Stella Chiamaka Anaba
Daniel Udofia Owu
Cardioprotective Effect of Peperomia pellucida against Doxorubicin-Induced Cardiotoxicity in Wistar Rats via Modulation of Electrocardiographic and Cardiac Biomarkers
Journal of Pharmacopuncture
captopril
cardiac troponin
cardiotoxicity
electrocardiograph
inflammation
peperomia pellucida
title Cardioprotective Effect of Peperomia pellucida against Doxorubicin-Induced Cardiotoxicity in Wistar Rats via Modulation of Electrocardiographic and Cardiac Biomarkers
title_full Cardioprotective Effect of Peperomia pellucida against Doxorubicin-Induced Cardiotoxicity in Wistar Rats via Modulation of Electrocardiographic and Cardiac Biomarkers
title_fullStr Cardioprotective Effect of Peperomia pellucida against Doxorubicin-Induced Cardiotoxicity in Wistar Rats via Modulation of Electrocardiographic and Cardiac Biomarkers
title_full_unstemmed Cardioprotective Effect of Peperomia pellucida against Doxorubicin-Induced Cardiotoxicity in Wistar Rats via Modulation of Electrocardiographic and Cardiac Biomarkers
title_short Cardioprotective Effect of Peperomia pellucida against Doxorubicin-Induced Cardiotoxicity in Wistar Rats via Modulation of Electrocardiographic and Cardiac Biomarkers
title_sort cardioprotective effect of peperomia pellucida against doxorubicin induced cardiotoxicity in wistar rats via modulation of electrocardiographic and cardiac biomarkers
topic captopril
cardiac troponin
cardiotoxicity
electrocardiograph
inflammation
peperomia pellucida
url http://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2024.27.4.297
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