RETRACTED: Mechanism of action of Asparagus officinalis extract against multiple myeloma using bioinformatics tools, in silico and in vitro study
Introduction: Asparagus (Asparagus officinalis) is a perennial flowering plant species. Its main components have tumor-prevention, immune system-enhancement, and anti-inflammation effects. Network pharmacology is a powerful approach that is being applied increasingly to research of herbal medicines....
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1076815/full |
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| author | Yanju Li Xu Yang Feiqing Wang Feiqing Wang Jianing Zhao Chike Zhang Dan Wu Bo Yang Rui Gao Peng Zhao Yun Zan Min Su Zhixu He Yang Liu Yang Liu Yang Liu Jishi Wang Dongxin Tang |
| author_facet | Yanju Li Xu Yang Feiqing Wang Feiqing Wang Jianing Zhao Chike Zhang Dan Wu Bo Yang Rui Gao Peng Zhao Yun Zan Min Su Zhixu He Yang Liu Yang Liu Yang Liu Jishi Wang Dongxin Tang |
| author_sort | Yanju Li |
| collection | DOAJ |
| description | Introduction: Asparagus (Asparagus officinalis) is a perennial flowering plant species. Its main components have tumor-prevention, immune system-enhancement, and anti-inflammation effects. Network pharmacology is a powerful approach that is being applied increasingly to research of herbal medicines. Herb identification, study of compound targets, network construction, and network analysis have been used to elucidate how herbal medicines work. However, the interaction of bioactive substances from asparagus with the targets involved in multiple myeloma (MM) has not been elucidated. We explored the mechanism of action of asparagus in MM through network pharmacology and experimental verification.Methods: The active ingredients and corresponding targets of asparagus were acquired from the Traditional Chinese Medicine System Pharmacology database, followed by identification of MM-related target genes using GeneCards and Online Mendelian Inheritance in Man databases, which were matched with the potential targets of asparagus. Potential targets were identified and a target network of traditional Chinese medicine was constructed. The STRING database and Cytoscape were utilized to create protein–protein interaction (PPI) networks and further screening of core targets.Results: The intersection of target genes and core target genes of the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) pathway was enriched, the top-five core target genes were selected, and the binding affinity of corresponding compounds to the top-five core targets was analyzed using molecular docking. Network pharmacology identified nine active components of asparagus from databases based on oral bioavailability and drug similarity, and predicted 157 potential targets related to asparagus. Enrichment analyses showed that “steroid receptor activity” and the “PI3K/AKT signaling pathway” were the most enriched biological process and signaling pathway, respectively. According to the top-10 core genes and targets of the PPI pathway, AKT1, interleukin (IL)-6, vascular endothelial growth factor (VEGF)A, MYC, and epidermal growth factor receptor (EGFR) were selected for molecular docking. The latter showed that five core targets of the PI3K/AKT signaling pathway could bind to quercetin, among which EGFR, IL-6, and MYC showed strong docking, and the diosgenin ligand could bind to VEGFA. Cell experiments showed that asparagus, through the PI3K/AKT/NF-κB pathway, inhibited the proliferation and migration of MM cells, and caused retardation and apoptosis of MM cells in the G0/G1 phase.Discussion: In this study, the anti-cancer activity of asparagus against MM was demonstrated using network pharmacology, and potential pharmacological mechanisms were inferred using in vitro experimental data. |
| format | Article |
| id | doaj-art-b65cc094f1e5430799bf2ed1df4788bb |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-b65cc094f1e5430799bf2ed1df4788bb2025-01-27T09:58:51ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-05-011410.3389/fphar.2023.10768151076815RETRACTED: Mechanism of action of Asparagus officinalis extract against multiple myeloma using bioinformatics tools, in silico and in vitro studyYanju Li0Xu Yang1Feiqing Wang2Feiqing Wang3Jianing Zhao4Chike Zhang5Dan Wu6Bo Yang7Rui Gao8Peng Zhao9Yun Zan10Min Su11Zhixu He12Yang Liu13Yang Liu14Yang Liu15Jishi Wang16Dongxin Tang17Department of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, ChinaClinical Medical Research Center, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaClinical Medical Research Center, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaAcademy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin City, ChinaClinical Medical Research Center, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaDepartment of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, ChinaClinical Medical Research Center, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaClinical Medical Research Center, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaDepartment of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, ChinaDepartment of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, ChinaDepartment of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, ChinaKey Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guizhou Medical University, Guiyang, Guizhou, ChinaKey Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guizhou Medical University, Guiyang, Guizhou, ChinaDepartment of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, ChinaClinical Medical Research Center, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaKey Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guizhou Medical University, Guiyang, Guizhou, ChinaDepartment of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, ChinaClinical Medical Research Center, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, ChinaIntroduction: Asparagus (Asparagus officinalis) is a perennial flowering plant species. Its main components have tumor-prevention, immune system-enhancement, and anti-inflammation effects. Network pharmacology is a powerful approach that is being applied increasingly to research of herbal medicines. Herb identification, study of compound targets, network construction, and network analysis have been used to elucidate how herbal medicines work. However, the interaction of bioactive substances from asparagus with the targets involved in multiple myeloma (MM) has not been elucidated. We explored the mechanism of action of asparagus in MM through network pharmacology and experimental verification.Methods: The active ingredients and corresponding targets of asparagus were acquired from the Traditional Chinese Medicine System Pharmacology database, followed by identification of MM-related target genes using GeneCards and Online Mendelian Inheritance in Man databases, which were matched with the potential targets of asparagus. Potential targets were identified and a target network of traditional Chinese medicine was constructed. The STRING database and Cytoscape were utilized to create protein–protein interaction (PPI) networks and further screening of core targets.Results: The intersection of target genes and core target genes of the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) pathway was enriched, the top-five core target genes were selected, and the binding affinity of corresponding compounds to the top-five core targets was analyzed using molecular docking. Network pharmacology identified nine active components of asparagus from databases based on oral bioavailability and drug similarity, and predicted 157 potential targets related to asparagus. Enrichment analyses showed that “steroid receptor activity” and the “PI3K/AKT signaling pathway” were the most enriched biological process and signaling pathway, respectively. According to the top-10 core genes and targets of the PPI pathway, AKT1, interleukin (IL)-6, vascular endothelial growth factor (VEGF)A, MYC, and epidermal growth factor receptor (EGFR) were selected for molecular docking. The latter showed that five core targets of the PI3K/AKT signaling pathway could bind to quercetin, among which EGFR, IL-6, and MYC showed strong docking, and the diosgenin ligand could bind to VEGFA. Cell experiments showed that asparagus, through the PI3K/AKT/NF-κB pathway, inhibited the proliferation and migration of MM cells, and caused retardation and apoptosis of MM cells in the G0/G1 phase.Discussion: In this study, the anti-cancer activity of asparagus against MM was demonstrated using network pharmacology, and potential pharmacological mechanisms were inferred using in vitro experimental data.https://www.frontiersin.org/articles/10.3389/fphar.2023.1076815/fullmultiple myelomaasparagusnetwork pharmacologymolecular dockingPI3K/Akt/NF-κB signaling pathway |
| spellingShingle | Yanju Li Xu Yang Feiqing Wang Feiqing Wang Jianing Zhao Chike Zhang Dan Wu Bo Yang Rui Gao Peng Zhao Yun Zan Min Su Zhixu He Yang Liu Yang Liu Yang Liu Jishi Wang Dongxin Tang RETRACTED: Mechanism of action of Asparagus officinalis extract against multiple myeloma using bioinformatics tools, in silico and in vitro study Frontiers in Pharmacology multiple myeloma asparagus network pharmacology molecular docking PI3K/Akt/NF-κB signaling pathway |
| title | RETRACTED: Mechanism of action of Asparagus officinalis extract against multiple myeloma using bioinformatics tools, in silico and in vitro study |
| title_full | RETRACTED: Mechanism of action of Asparagus officinalis extract against multiple myeloma using bioinformatics tools, in silico and in vitro study |
| title_fullStr | RETRACTED: Mechanism of action of Asparagus officinalis extract against multiple myeloma using bioinformatics tools, in silico and in vitro study |
| title_full_unstemmed | RETRACTED: Mechanism of action of Asparagus officinalis extract against multiple myeloma using bioinformatics tools, in silico and in vitro study |
| title_short | RETRACTED: Mechanism of action of Asparagus officinalis extract against multiple myeloma using bioinformatics tools, in silico and in vitro study |
| title_sort | retracted mechanism of action of asparagus officinalis extract against multiple myeloma using bioinformatics tools in silico and in vitro study |
| topic | multiple myeloma asparagus network pharmacology molecular docking PI3K/Akt/NF-κB signaling pathway |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1076815/full |
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