Effect of IL-2 T-330G polymorphism on markers of systemic inflammation, intestinal permeability and vascular regulation in post-COVID patients
Recent studies suggest a role of genetic factors, i.e., polymorphisms of genes controlling the main immune components of antiviral response, and the risk of COVID-19 infection, severe course and lethal outcomes of the disease, as well as development of clinical or laboratory changes in the post-COVI...
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2025-03-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/3024 |
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| author | I. A. Yatskov V. A. Beloglazov E. S. Ageeva R. N. Ablaeva A. A. Zhukova I. G. Onuchina |
| author_facet | I. A. Yatskov V. A. Beloglazov E. S. Ageeva R. N. Ablaeva A. A. Zhukova I. G. Onuchina |
| author_sort | I. A. Yatskov |
| collection | DOAJ |
| description | Recent studies suggest a role of genetic factors, i.e., polymorphisms of genes controlling the main immune components of antiviral response, and the risk of COVID-19 infection, severe course and lethal outcomes of the disease, as well as development of clinical or laboratory changes in the post-COVID period. Interleukin-2 (IL-2) plays an important role in immunopathology of COVID-19. However, there are only few data on the impact of single nucleotide polymorphisms, e.g., IL-2 330T/G (rs2069762) gene variant on molecular changes during the post-COVID period. The aim of our study was to investigate differences in systemic inflammation markers, intestinal permeability and vascular regulation in patients with post-COVID syndrome, and single nucleotide polymorphisms (SNPs) of the IL-2 330T/G gene. Fiftyfour patients (28 females (51.85%) and 26 males (48.15%), mean age 45.6±6.14 years) who suffered with COVID-19 were included into the study. The patients were tested for IL-2 T-330G polymorphism by allelespecific polymerase chain reaction (PCR) with electrophoretic detection of products. PCR was performed using the IL-2 T-330G kit (LLC "Litech", Russia). The contents of C-reactive protein (CRP, mg/L), lipopolysaccharide-binding protein (LBP, ng/mL), tissue plasminogen activator (tPA, ng/mL), zonulin (ng/ mL), endothelin-1 (pg/mL), and angiotensin-2 (pg/mL) in blood plasma were determined by ELISA test manufactured by Cloud Clone Corp. (Wuhan, Hubei, China). The patients harboring a homozygous GG variant of T-330G IL-2 gene polymorphism showed significantly lower CRP levels than in the heterozygous TG group (p = 0.013), and TT homozygous group (p = 0.039). The tPA levels were significantly higher in the GG homozygote group compared to TT homozygotes (p = 0.017). The highest zonulin values were recorded in the TG heterozygote group, compared to TT homozygotes (p = 0.013). The highest angiotensin-2 values were found in the homozygous TT group (p < 0.05). No significant variations of LBP and endothelin-1 were registered between the studied groups. The T-330G polymorphism of IL-2 gene is, therefore, associated with some molecular changes in the post-COVID period, which may potentially influence both clinical manifestations of post-COVID syndrome, and its long-term consequences in future. Further in-depth studies of T-330G effects upon activity of the IL-2 gene and related molecular events is necessary in order to understand the clinical aspects of post-COVID syndrome. |
| format | Article |
| id | doaj-art-b656ac5326834e25944d705ab0f0a7b8 |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2025-03-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
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| series | Медицинская иммунология |
| spelling | doaj-art-b656ac5326834e25944d705ab0f0a7b82025-08-20T03:38:01ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2025-03-0127241742210.15789/1563-0625-EOI-30241979Effect of IL-2 T-330G polymorphism on markers of systemic inflammation, intestinal permeability and vascular regulation in post-COVID patientsI. A. Yatskov0V. A. Beloglazov1E. S. Ageeva2R. N. Ablaeva3A. A. Zhukova4I. G. Onuchina5S. Georgievsky Medical Institute, V. Vernadsky Crimean Federal UniversityS. Georgievsky Medical Institute, V. Vernadsky Crimean Federal UniversityS. Georgievsky Medical Institute, V. Vernadsky Crimean Federal UniversityS. Georgievsky Medical Institute, V. Vernadsky Crimean Federal UniversityS. Georgievsky Medical Institute, V. Vernadsky Crimean Federal UniversityS. Georgievsky Medical Institute, V. Vernadsky Crimean Federal UniversityRecent studies suggest a role of genetic factors, i.e., polymorphisms of genes controlling the main immune components of antiviral response, and the risk of COVID-19 infection, severe course and lethal outcomes of the disease, as well as development of clinical or laboratory changes in the post-COVID period. Interleukin-2 (IL-2) plays an important role in immunopathology of COVID-19. However, there are only few data on the impact of single nucleotide polymorphisms, e.g., IL-2 330T/G (rs2069762) gene variant on molecular changes during the post-COVID period. The aim of our study was to investigate differences in systemic inflammation markers, intestinal permeability and vascular regulation in patients with post-COVID syndrome, and single nucleotide polymorphisms (SNPs) of the IL-2 330T/G gene. Fiftyfour patients (28 females (51.85%) and 26 males (48.15%), mean age 45.6±6.14 years) who suffered with COVID-19 were included into the study. The patients were tested for IL-2 T-330G polymorphism by allelespecific polymerase chain reaction (PCR) with electrophoretic detection of products. PCR was performed using the IL-2 T-330G kit (LLC "Litech", Russia). The contents of C-reactive protein (CRP, mg/L), lipopolysaccharide-binding protein (LBP, ng/mL), tissue plasminogen activator (tPA, ng/mL), zonulin (ng/ mL), endothelin-1 (pg/mL), and angiotensin-2 (pg/mL) in blood plasma were determined by ELISA test manufactured by Cloud Clone Corp. (Wuhan, Hubei, China). The patients harboring a homozygous GG variant of T-330G IL-2 gene polymorphism showed significantly lower CRP levels than in the heterozygous TG group (p = 0.013), and TT homozygous group (p = 0.039). The tPA levels were significantly higher in the GG homozygote group compared to TT homozygotes (p = 0.017). The highest zonulin values were recorded in the TG heterozygote group, compared to TT homozygotes (p = 0.013). The highest angiotensin-2 values were found in the homozygous TT group (p < 0.05). No significant variations of LBP and endothelin-1 were registered between the studied groups. The T-330G polymorphism of IL-2 gene is, therefore, associated with some molecular changes in the post-COVID period, which may potentially influence both clinical manifestations of post-COVID syndrome, and its long-term consequences in future. Further in-depth studies of T-330G effects upon activity of the IL-2 gene and related molecular events is necessary in order to understand the clinical aspects of post-COVID syndrome.https://www.mimmun.ru/mimmun/article/view/3024polymorphismsars-cov-2new coronavirus infectionil-2t-330g polymorphismcrppermeability |
| spellingShingle | I. A. Yatskov V. A. Beloglazov E. S. Ageeva R. N. Ablaeva A. A. Zhukova I. G. Onuchina Effect of IL-2 T-330G polymorphism on markers of systemic inflammation, intestinal permeability and vascular regulation in post-COVID patients Медицинская иммунология polymorphism sars-cov-2 new coronavirus infection il-2 t-330g polymorphism crp permeability |
| title | Effect of IL-2 T-330G polymorphism on markers of systemic inflammation, intestinal permeability and vascular regulation in post-COVID patients |
| title_full | Effect of IL-2 T-330G polymorphism on markers of systemic inflammation, intestinal permeability and vascular regulation in post-COVID patients |
| title_fullStr | Effect of IL-2 T-330G polymorphism on markers of systemic inflammation, intestinal permeability and vascular regulation in post-COVID patients |
| title_full_unstemmed | Effect of IL-2 T-330G polymorphism on markers of systemic inflammation, intestinal permeability and vascular regulation in post-COVID patients |
| title_short | Effect of IL-2 T-330G polymorphism on markers of systemic inflammation, intestinal permeability and vascular regulation in post-COVID patients |
| title_sort | effect of il 2 t 330g polymorphism on markers of systemic inflammation intestinal permeability and vascular regulation in post covid patients |
| topic | polymorphism sars-cov-2 new coronavirus infection il-2 t-330g polymorphism crp permeability |
| url | https://www.mimmun.ru/mimmun/article/view/3024 |
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