Optimization, purification and antioxidant potential of polyphenol ultrasonic-assisted extraction from pecan 'Shaoxing' green husk
Abstract In this study, ultrasonic-assisted extraction method was used to extract polyphenols from pecan 'Shaoxing' green husk. The optimization of extraction technology involved both single-factor and response surface methodology, while the purification technology was determined to refine...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-03-01
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| Series: | Food Production, Processing and Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43014-024-00296-8 |
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| Summary: | Abstract In this study, ultrasonic-assisted extraction method was used to extract polyphenols from pecan 'Shaoxing' green husk. The optimization of extraction technology involved both single-factor and response surface methodology, while the purification technology was determined to refine the crude polyphenol extract. Assessment of radical scavenging activity of pecan 'Shaoxing' green husk polyphenols on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydroxyl radical (•OH), and superoxide anion (O 2 •− ) was conducted with L-ascorbic acid (Vc) as the control. Results showed that optimal extraction conditions, including ethanol solvent, material-liquid ratio of 1:15, 58% ethanol volume fraction, 60 min ultrasonic time, 160 W ultrasonic power, and 57 ℃ ultrasonic temperature, yielded a polyphenol content of 218.62 mg/g. Macroporous resin D-101 was selected for polyphenol purification with optimized parameters: 2 mg/mL loading concentration, pH = 4, 2 mL/min loading flow rate, elution with 70% ethanol volume fraction, and 3 mL/min elution flow rate. The purity of polyphenols increased from 31.45 to 69.34%. At the pecan 'Shaoxing' green husk polyphenol concentration of 0.9 mg/mL, DPPH, ABTS, O 2 •− , and •OH radical scavenging activity were measured at 95.36, 99.4, 50.92, and 51.89%, respectively, indicating significant antioxidant activity. LC–MS analysis detected 24 polyphenol components in pecan 'Shaoxing' green husk, with relatively higher rutin and proanthocyanidin B2 contents compared to other components. Graphical Abstract |
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| ISSN: | 2661-8974 |