Virulence and Antimicrobial Resistance in Campylobacter spp. from a Peruvian Pediatric Cohort
The presence of virulence factors (VFs) and mechanisms of quinolones and macrolide resistance was analyzed in Campylobacter spp. from a pediatric cohort study in Lima. In 149 isolates (39 Campylobacter jejuni and 24 Campylobacter coli from diarrheic cases; 57 C. jejuni and 29 C. coli from controls),...
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Wiley
2017-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2017/7848926 |
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| author | Angela Lluque Maribel Riveros Ana Prada Theresa J. Ochoa Joaquim Ruiz |
| author_facet | Angela Lluque Maribel Riveros Ana Prada Theresa J. Ochoa Joaquim Ruiz |
| author_sort | Angela Lluque |
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| description | The presence of virulence factors (VFs) and mechanisms of quinolones and macrolide resistance was analyzed in Campylobacter spp. from a pediatric cohort study in Lima. In 149 isolates (39 Campylobacter jejuni and 24 Campylobacter coli from diarrheic cases; 57 C. jejuni and 29 C. coli from controls), the presence of the cdtABC and cadF genes and iam marker was established. Nalidixic acid, ciprofloxacin, erythromycin, and azithromycin susceptibilities were established in 115 isolates and tetracycline-susceptibility was established in 100 isolates. The presence of mutations in the gyrA, parC, and 23S rRNA genes was determined. The cadF gene and all genes from the cdtABC operon were significantly more frequent among C. jejuni (P<0.0001); the iam marker was more frequent in C. coli (P<0.0001). No differences were observed in VFs between cases and controls. Almost all isolates were tetracycline-resistant; nalidixic acid and ciprofloxacin resistance reached levels of 90.4% and 88.7%, respectively. Resistance to macrolides was 13% (C. jejuni 4.3%; C. coli 26.1%). Resistance to ciprofloxacin was related to GyrA Thr86 substitutions, while 13 of 15 macrolide-resistant isolates possessed a 23S rRNA mutation (A2075G). Differences in the presence of VFs and alarming levels of resistance to tested antimicrobial agents were observed among C. jejuni and C. coli. |
| format | Article |
| id | doaj-art-b64e84b357eb42af933e5024af34cb32 |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2017-01-01 |
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| spelling | doaj-art-b64e84b357eb42af933e5024af34cb322025-02-03T06:05:58ZengWileyScientifica2090-908X2017-01-01201710.1155/2017/78489267848926Virulence and Antimicrobial Resistance in Campylobacter spp. from a Peruvian Pediatric CohortAngela Lluque0Maribel Riveros1Ana Prada2Theresa J. Ochoa3Joaquim Ruiz4Instituto de Medicina Tropical, Universidad Peruana Cayetano Heredia, Lima, PeruInstituto de Medicina Tropical, Universidad Peruana Cayetano Heredia, Lima, PeruInstituto de Medicina Tropical, Universidad Peruana Cayetano Heredia, Lima, PeruInstituto de Medicina Tropical, Universidad Peruana Cayetano Heredia, Lima, PeruISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic, Universitat de Barcelona, Barcelona, SpainThe presence of virulence factors (VFs) and mechanisms of quinolones and macrolide resistance was analyzed in Campylobacter spp. from a pediatric cohort study in Lima. In 149 isolates (39 Campylobacter jejuni and 24 Campylobacter coli from diarrheic cases; 57 C. jejuni and 29 C. coli from controls), the presence of the cdtABC and cadF genes and iam marker was established. Nalidixic acid, ciprofloxacin, erythromycin, and azithromycin susceptibilities were established in 115 isolates and tetracycline-susceptibility was established in 100 isolates. The presence of mutations in the gyrA, parC, and 23S rRNA genes was determined. The cadF gene and all genes from the cdtABC operon were significantly more frequent among C. jejuni (P<0.0001); the iam marker was more frequent in C. coli (P<0.0001). No differences were observed in VFs between cases and controls. Almost all isolates were tetracycline-resistant; nalidixic acid and ciprofloxacin resistance reached levels of 90.4% and 88.7%, respectively. Resistance to macrolides was 13% (C. jejuni 4.3%; C. coli 26.1%). Resistance to ciprofloxacin was related to GyrA Thr86 substitutions, while 13 of 15 macrolide-resistant isolates possessed a 23S rRNA mutation (A2075G). Differences in the presence of VFs and alarming levels of resistance to tested antimicrobial agents were observed among C. jejuni and C. coli.http://dx.doi.org/10.1155/2017/7848926 |
| spellingShingle | Angela Lluque Maribel Riveros Ana Prada Theresa J. Ochoa Joaquim Ruiz Virulence and Antimicrobial Resistance in Campylobacter spp. from a Peruvian Pediatric Cohort Scientifica |
| title | Virulence and Antimicrobial Resistance in Campylobacter spp. from a Peruvian Pediatric Cohort |
| title_full | Virulence and Antimicrobial Resistance in Campylobacter spp. from a Peruvian Pediatric Cohort |
| title_fullStr | Virulence and Antimicrobial Resistance in Campylobacter spp. from a Peruvian Pediatric Cohort |
| title_full_unstemmed | Virulence and Antimicrobial Resistance in Campylobacter spp. from a Peruvian Pediatric Cohort |
| title_short | Virulence and Antimicrobial Resistance in Campylobacter spp. from a Peruvian Pediatric Cohort |
| title_sort | virulence and antimicrobial resistance in campylobacter spp from a peruvian pediatric cohort |
| url | http://dx.doi.org/10.1155/2017/7848926 |
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