Rotational Dynamics of Optically Trapped Human Spermatozoa
Introduction. Optical trapping is a laser-based method for probing the physiological and mechanical properties of cells in a noninvasive manner. As sperm motility is an important criterion for assessing the male fertility potential, this technique is used to study sperm cell motility behavior and ro...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/154367 |
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| author | Elavarasan Subramani Himanish Basu Shyam Thangaraju Sucheta Dandekar Deepak Mathur Koel Chaudhury |
| author_facet | Elavarasan Subramani Himanish Basu Shyam Thangaraju Sucheta Dandekar Deepak Mathur Koel Chaudhury |
| author_sort | Elavarasan Subramani |
| collection | DOAJ |
| description | Introduction. Optical trapping is a laser-based method for probing the physiological and mechanical properties of cells in a noninvasive manner. As sperm motility is an important criterion for assessing the male fertility potential, this technique is used to study sperm cell motility behavior and rotational dynamics. Methods and Patients. An integrated optical system with near-infrared laser beam has been used to analyze rotational dynamics of live sperm cells from oligozoospermic and asthenozoospermic cases and compared with controls. Results. The linear, translational motion of the sperm is converted into rotational motion on being optically trapped, without causing any adverse effect on spermatozoa. The rotational speed of sperm cells from infertile men is observed to be significantly less as compared to controls. Conclusions. Distinguishing normal and abnormal sperm cells on the basis of beat frequency above 5.6 Hz may be an important step in modern reproductive biology to sort and select good quality spermatozoa. The application of laser-assisted technique in biology has the potential to be a valuable tool for assessment of sperm fertilization capacity for improving assisted reproductive technology. |
| format | Article |
| id | doaj-art-b64b19280b3e4b65ab9a00566fcbf820 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-b64b19280b3e4b65ab9a00566fcbf8202025-02-03T01:01:00ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/154367154367Rotational Dynamics of Optically Trapped Human SpermatozoaElavarasan Subramani0Himanish Basu1Shyam Thangaraju2Sucheta Dandekar3Deepak Mathur4Koel Chaudhury5School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721 302, IndiaTata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, IndiaSchool of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721 302, IndiaDepartment of Biochemistry, Seth GS Medical College and KEM Hospital, Mumbai 400 012, IndiaTata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, IndiaSchool of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721 302, IndiaIntroduction. Optical trapping is a laser-based method for probing the physiological and mechanical properties of cells in a noninvasive manner. As sperm motility is an important criterion for assessing the male fertility potential, this technique is used to study sperm cell motility behavior and rotational dynamics. Methods and Patients. An integrated optical system with near-infrared laser beam has been used to analyze rotational dynamics of live sperm cells from oligozoospermic and asthenozoospermic cases and compared with controls. Results. The linear, translational motion of the sperm is converted into rotational motion on being optically trapped, without causing any adverse effect on spermatozoa. The rotational speed of sperm cells from infertile men is observed to be significantly less as compared to controls. Conclusions. Distinguishing normal and abnormal sperm cells on the basis of beat frequency above 5.6 Hz may be an important step in modern reproductive biology to sort and select good quality spermatozoa. The application of laser-assisted technique in biology has the potential to be a valuable tool for assessment of sperm fertilization capacity for improving assisted reproductive technology.http://dx.doi.org/10.1155/2014/154367 |
| spellingShingle | Elavarasan Subramani Himanish Basu Shyam Thangaraju Sucheta Dandekar Deepak Mathur Koel Chaudhury Rotational Dynamics of Optically Trapped Human Spermatozoa The Scientific World Journal |
| title | Rotational Dynamics of Optically Trapped Human Spermatozoa |
| title_full | Rotational Dynamics of Optically Trapped Human Spermatozoa |
| title_fullStr | Rotational Dynamics of Optically Trapped Human Spermatozoa |
| title_full_unstemmed | Rotational Dynamics of Optically Trapped Human Spermatozoa |
| title_short | Rotational Dynamics of Optically Trapped Human Spermatozoa |
| title_sort | rotational dynamics of optically trapped human spermatozoa |
| url | http://dx.doi.org/10.1155/2014/154367 |
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