Peanut Growth Promotion and Biocontrol of Blight by <i>Sclerotium rolfsii</i> with Rhizosphere Bacterium, <i>Bacillus siamensis</i> YB-1632

Peanut Growth Promotion and Biocontrol of Blight by <i>Sclerotium rolfsii</i> with Rhizosphere Bacterium, <i>Bacillus siamensis</i> YB-1632

A total of 34 strains of bacteria were isolated from peanut rhizosphere soil, and all showed some in vitro inhibition of the pathogen <i>Sclerotium rolfsii</i> in co-culture. Strain YB-1632 produced the highest level of inhibition and also produced relatively high levels of biofilm in cu...

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Bibliographic Details
Main Authors: Yinghang Chang, Qianqian Dong, Limei Zhang, Paul H. Goodwin, Wen Xu, Mingcong Xia, Jie Zhang, Runhong Sun, Chao Wu, Kun Wu, Shuxia Xu, Lirong Yang
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Agronomy
Subjects:
induced systemic resistance
peanut sclerotium blight
plant growth-promoting rhizobacterium
rhizosphere
Online Access:https://www.mdpi.com/2073-4395/15/3/568
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Summary:A total of 34 strains of bacteria were isolated from peanut rhizosphere soil, and all showed some in vitro inhibition of the pathogen <i>Sclerotium rolfsii</i> in co-culture. Strain YB-1632 produced the highest level of inhibition and also produced relatively high levels of biofilm in culture. Cell-free culture extracts and volatiles from it were also inhibitory to <i>S. rolfsii</i>. Based on 16S rDNA, <i>gyrA</i>, and <i>gyrB</i> sequences, it was identified as <i>Bacillus siamensis</i>. In the greenhouse, seed treatment resulted in a level of control of peanut sclerotium blight (PSB) comparable to that of a standard fungicide seed treatment. In addition to its antifungal activity, YB-1632 could induce disease resistance in peanut seedlings based on increasing peanut defense enzyme activities and gene expression. The priming of defense gene expression against a necrotrophic pathogen is consistent with Induced Systemic Resistance (ISR). In addition, YB-1632 produced enzyme activities in culture associated with root colonization and plant growth promotion. In the greenhouse, it increased peanut seedling growth, indicating the YB-1632 is a plant growth-promoting rhizobacterium (PGPR). In summary, YB-1632 is a promising novel PSB biocontrol agent and PGPR of peanut.
ISSN:2073-4395

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