Novel genetic loci and a functional gene Gm18GRSC3 conferring SMV-SC3 resistance in soybean
Soybean mosaic virus (SMV) is a worldwide disease significantly decreasing soybean yield and seed quality. In this study, a genome-wide association study (GWAS) for SMV-SC3 resistance was conducted by using a deep re-sequencing dataset of 547 soybean accessions. A total of 11,405 SNPs and 1566 InDel...
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KeAi Communications Co., Ltd.
2025-08-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514125001394 |
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| author | Jiahao Chu Wenlong Li Zhenqi Shao Zhanwu Yang Xinzhu Xing Hua Zhang Rui Tian Huantao Zhang Xihuan Li Caiying Zhang |
| author_facet | Jiahao Chu Wenlong Li Zhenqi Shao Zhanwu Yang Xinzhu Xing Hua Zhang Rui Tian Huantao Zhang Xihuan Li Caiying Zhang |
| author_sort | Jiahao Chu |
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| description | Soybean mosaic virus (SMV) is a worldwide disease significantly decreasing soybean yield and seed quality. In this study, a genome-wide association study (GWAS) for SMV-SC3 resistance was conducted by using a deep re-sequencing dataset of 547 soybean accessions. A total of 11,405 SNPs and 1566 InDels were significantly associated with disease index (DI) at seedling stage and eight yield- and seed quality-related traits to SC3 resistance under eight environments. Among these genetic loci, 952 SNPs and 118 InDels were firstly identified to control SC3 resistance, and 52.42% and 42.37% of them were pleiotropic loci across multiple environments. Notably, the 8.47–8.89 Mb genomic region on chromosome 18 was firstly discovvered to associate with DI at seedling stage and four related traits at adult stage across multiple environments. Furthermore, the causal gene Gm18GRSC3 was identified and validated in this stable and pleiotropic locus for resistance to SC3 via positive and negative transgenic strategies. Overexpression of Gm18GRSC3 significantly decreased the accumulation of SC3 in transgenic soybean hairy roots, while silencing of Gm18GRSC3 significantly increased SC3 accumulation in soybean leaves. A functional marker, FM18GSC3, was developed based on the allelic variation of Gm18GRSC3, and the detection efficiency reached to 76% in another 100 soybean accessions. These findings provide valuable genetic loci and a functional gene for the improvement of SMV resistance in soybean. |
| format | Article |
| id | doaj-art-b6478c7837aa4c5bbc1aa2175a7e08ea |
| institution | Kabale University |
| issn | 2214-5141 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj-art-b6478c7837aa4c5bbc1aa2175a7e08ea2025-08-22T04:56:32ZengKeAi Communications Co., Ltd.Crop Journal2214-51412025-08-011341127113610.1016/j.cj.2025.05.012Novel genetic loci and a functional gene Gm18GRSC3 conferring SMV-SC3 resistance in soybeanJiahao Chu0Wenlong Li1Zhenqi Shao2Zhanwu Yang3Xinzhu Xing4Hua Zhang5Rui Tian6Huantao Zhang7Xihuan Li8Caiying Zhang9State Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding 071001, Hebei, China; Hebei Key Laboratory of Crop Stress Biology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, Hebei, ChinaState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding 071001, Hebei, ChinaState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding 071001, Hebei, ChinaState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding 071001, Hebei, ChinaState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding 071001, Hebei, ChinaState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding 071001, Hebei, ChinaState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding 071001, Hebei, ChinaState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding 071001, Hebei, ChinaState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding 071001, Hebei, China; Corresponding authors.State Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Hebei Agricultural University, Baoding 071001, Hebei, China; Corresponding authors.Soybean mosaic virus (SMV) is a worldwide disease significantly decreasing soybean yield and seed quality. In this study, a genome-wide association study (GWAS) for SMV-SC3 resistance was conducted by using a deep re-sequencing dataset of 547 soybean accessions. A total of 11,405 SNPs and 1566 InDels were significantly associated with disease index (DI) at seedling stage and eight yield- and seed quality-related traits to SC3 resistance under eight environments. Among these genetic loci, 952 SNPs and 118 InDels were firstly identified to control SC3 resistance, and 52.42% and 42.37% of them were pleiotropic loci across multiple environments. Notably, the 8.47–8.89 Mb genomic region on chromosome 18 was firstly discovvered to associate with DI at seedling stage and four related traits at adult stage across multiple environments. Furthermore, the causal gene Gm18GRSC3 was identified and validated in this stable and pleiotropic locus for resistance to SC3 via positive and negative transgenic strategies. Overexpression of Gm18GRSC3 significantly decreased the accumulation of SC3 in transgenic soybean hairy roots, while silencing of Gm18GRSC3 significantly increased SC3 accumulation in soybean leaves. A functional marker, FM18GSC3, was developed based on the allelic variation of Gm18GRSC3, and the detection efficiency reached to 76% in another 100 soybean accessions. These findings provide valuable genetic loci and a functional gene for the improvement of SMV resistance in soybean.http://www.sciencedirect.com/science/article/pii/S2214514125001394SoybeanSMV-SC3GWASGenetic lociFunctional marker |
| spellingShingle | Jiahao Chu Wenlong Li Zhenqi Shao Zhanwu Yang Xinzhu Xing Hua Zhang Rui Tian Huantao Zhang Xihuan Li Caiying Zhang Novel genetic loci and a functional gene Gm18GRSC3 conferring SMV-SC3 resistance in soybean Crop Journal Soybean SMV-SC3 GWAS Genetic loci Functional marker |
| title | Novel genetic loci and a functional gene Gm18GRSC3 conferring SMV-SC3 resistance in soybean |
| title_full | Novel genetic loci and a functional gene Gm18GRSC3 conferring SMV-SC3 resistance in soybean |
| title_fullStr | Novel genetic loci and a functional gene Gm18GRSC3 conferring SMV-SC3 resistance in soybean |
| title_full_unstemmed | Novel genetic loci and a functional gene Gm18GRSC3 conferring SMV-SC3 resistance in soybean |
| title_short | Novel genetic loci and a functional gene Gm18GRSC3 conferring SMV-SC3 resistance in soybean |
| title_sort | novel genetic loci and a functional gene gm18grsc3 conferring smv sc3 resistance in soybean |
| topic | Soybean SMV-SC3 GWAS Genetic loci Functional marker |
| url | http://www.sciencedirect.com/science/article/pii/S2214514125001394 |
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