A Turn-On Fluorescence Sensor Based on Guest-Induced Luminescence Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 for the Detection of Organophosphorus Pesticides

Luminescent metal–organic frameworks (MOFs) are used for the detection of organophosphorus pesticides (OPs) due to their large surface area and pore volume as well as their special optical properties. However, most self-luminescent MOFs are not only complex to synthesize and unstable in water but al...

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Main Authors: Jun Li, Jianlan Deng, Qian Tao, Chenyu Yan, Yuxuan Liu, Jianxiao Yang, Zhong Cao
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/30/15/3130
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author Jun Li
Jianlan Deng
Qian Tao
Chenyu Yan
Yuxuan Liu
Jianxiao Yang
Zhong Cao
author_facet Jun Li
Jianlan Deng
Qian Tao
Chenyu Yan
Yuxuan Liu
Jianxiao Yang
Zhong Cao
author_sort Jun Li
collection DOAJ
description Luminescent metal–organic frameworks (MOFs) are used for the detection of organophosphorus pesticides (OPs) due to their large surface area and pore volume as well as their special optical properties. However, most self-luminescent MOFs are not only complex to synthesize and unstable in water but also feature a “turn-off” sensing system, which has highly restricted their practical applications in OP detection. Herein, a “turn-on” fluorescence sensor based on the guest-induced luminescence MOF Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 was constructed, which realized the sensitive detection of OPs through a dual-enzyme system for the first time. Compared with self-luminescent MOFs, Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 was not only more easily synthesized but also had higher chemical and photostability in water. In this strategy, by means of the hydrolysis of AChE and ChOx, H<sub>2</sub>O<sub>2</sub> will be produced, which can oxidize Fe<sup>2+</sup> to Fe<sup>3+</sup>, thereby quenching the fluorescence of Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66. In the presence of OPs, the activity of AChE can be inhibited, resulting in the inability to generate H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>, which will turn on the fluorescence signal of Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66. As a result, the Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 sensing system not only had high sensitivity for OPs detection but also possessed a satisfactory detection recovery rate for parathion-methyl in real samples, which provides a new approach for OP detection in food safety as well as environmental monitoring.
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spelling doaj-art-b63d2257bef84ab8af3a32d5e604521c2025-08-20T03:36:35ZengMDPI AGMolecules1420-30492025-07-013015313010.3390/molecules30153130A Turn-On Fluorescence Sensor Based on Guest-Induced Luminescence Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 for the Detection of Organophosphorus PesticidesJun Li0Jianlan Deng1Qian Tao2Chenyu Yan3Yuxuan Liu4Jianxiao Yang5Zhong Cao6Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Pharmaceutical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaHunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Pharmaceutical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaHunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Pharmaceutical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaHunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Pharmaceutical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaHunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Pharmaceutical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaHunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology, College of Materials Science and Engineering, Hunan University, Changsha 410082, ChinaHunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Pharmaceutical Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaLuminescent metal–organic frameworks (MOFs) are used for the detection of organophosphorus pesticides (OPs) due to their large surface area and pore volume as well as their special optical properties. However, most self-luminescent MOFs are not only complex to synthesize and unstable in water but also feature a “turn-off” sensing system, which has highly restricted their practical applications in OP detection. Herein, a “turn-on” fluorescence sensor based on the guest-induced luminescence MOF Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 was constructed, which realized the sensitive detection of OPs through a dual-enzyme system for the first time. Compared with self-luminescent MOFs, Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 was not only more easily synthesized but also had higher chemical and photostability in water. In this strategy, by means of the hydrolysis of AChE and ChOx, H<sub>2</sub>O<sub>2</sub> will be produced, which can oxidize Fe<sup>2+</sup> to Fe<sup>3+</sup>, thereby quenching the fluorescence of Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66. In the presence of OPs, the activity of AChE can be inhibited, resulting in the inability to generate H<sub>2</sub>O<sub>2</sub> and Fe<sup>3+</sup>, which will turn on the fluorescence signal of Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66. As a result, the Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 sensing system not only had high sensitivity for OPs detection but also possessed a satisfactory detection recovery rate for parathion-methyl in real samples, which provides a new approach for OP detection in food safety as well as environmental monitoring.https://www.mdpi.com/1420-3049/30/15/3130organophosphorus pesticidesguest-induced luminescent MOF“turn-on” fluorescence sensor
spellingShingle Jun Li
Jianlan Deng
Qian Tao
Chenyu Yan
Yuxuan Liu
Jianxiao Yang
Zhong Cao
A Turn-On Fluorescence Sensor Based on Guest-Induced Luminescence Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 for the Detection of Organophosphorus Pesticides
Molecules
organophosphorus pesticides
guest-induced luminescent MOF
“turn-on” fluorescence sensor
title A Turn-On Fluorescence Sensor Based on Guest-Induced Luminescence Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 for the Detection of Organophosphorus Pesticides
title_full A Turn-On Fluorescence Sensor Based on Guest-Induced Luminescence Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 for the Detection of Organophosphorus Pesticides
title_fullStr A Turn-On Fluorescence Sensor Based on Guest-Induced Luminescence Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 for the Detection of Organophosphorus Pesticides
title_full_unstemmed A Turn-On Fluorescence Sensor Based on Guest-Induced Luminescence Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 for the Detection of Organophosphorus Pesticides
title_short A Turn-On Fluorescence Sensor Based on Guest-Induced Luminescence Ru(bpy)<sub>3</sub><sup>2+</sup>@UiO-66 for the Detection of Organophosphorus Pesticides
title_sort turn on fluorescence sensor based on guest induced luminescence ru bpy sub 3 sub sup 2 sup uio 66 for the detection of organophosphorus pesticides
topic organophosphorus pesticides
guest-induced luminescent MOF
“turn-on” fluorescence sensor
url https://www.mdpi.com/1420-3049/30/15/3130
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