Transcriptomic Profiling of Thermotolerant <i>Sarcomyxa edulis</i> PQ650759 Reveals the Key Genes and Pathways During Fruiting Body Formation
<i>Sarcomyxa edulis</i> is a characteristic low-temperature, edible mushroom in Northeast China. It has a delicious taste and rich nutritional and medicinal value. <i>S. edulis</i> can undergo explosive fruiting, neat fruiting, and unified harvesting, making it suitable for f...
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2025-06-01
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| author | Zitong Liu Minglei Li Hongyu Ma Fei Wang Lei Shi Jinhe Wang Chunge Sheng Peng Zhang Haiyang Yu Jing Zhao Yanfeng Wang |
| author_facet | Zitong Liu Minglei Li Hongyu Ma Fei Wang Lei Shi Jinhe Wang Chunge Sheng Peng Zhang Haiyang Yu Jing Zhao Yanfeng Wang |
| author_sort | Zitong Liu |
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| description | <i>Sarcomyxa edulis</i> is a characteristic low-temperature, edible mushroom in Northeast China. It has a delicious taste and rich nutritional and medicinal value. <i>S. edulis</i> can undergo explosive fruiting, neat fruiting, and unified harvesting, making it suitable for factory production. The molecular mechanisms underlying fruiting body development in <i>S. edulis</i> remain poorly understood. This study employed transcriptome analysis to compare the post-ripening mycelium (NPM) and primordial fruiting bodies (PRMs) of the thermostable <i>S. edulis</i> strain PQ650759, which uniquely forms primordia under constant temperature. A total of 4862 differentially expressed genes (DEGs) (|log2(fold change)| ≥ 1) were identified and found to be predominantly enriched in biological processes such as cell wall organization, DNA replication, and carbohydrate metabolism. KEGG pathway analysis revealed significant enrichment in 20 metabolic pathways, including mismatch repair, yeast cell cycle, and starch/sucrose metabolism. Ten candidate genes (e.g., SKP1, MRE11, GPI) linked to cell cycle regulation, DNA repair, and energy metabolism were randomly selected and prioritized for functional analysis. Quantitative PCR validation confirmed the reliability of transcriptome data, with expression trends consistent across both methods. Our findings provide critical insights into the molecular regulation of fruiting body development in <i>S. edulis</i> and establish a foundation for future mechanistic studies and strain optimization in industrial cultivation. |
| format | Article |
| id | doaj-art-b63cbb8cc6b6434ca638accdd96e4b27 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-06-01 |
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| series | Journal of Fungi |
| spelling | doaj-art-b63cbb8cc6b6434ca638accdd96e4b272025-08-20T03:58:30ZengMDPI AGJournal of Fungi2309-608X2025-06-0111748410.3390/jof11070484Transcriptomic Profiling of Thermotolerant <i>Sarcomyxa edulis</i> PQ650759 Reveals the Key Genes and Pathways During Fruiting Body FormationZitong Liu0Minglei Li1Hongyu Ma2Fei Wang3Lei Shi4Jinhe Wang5Chunge Sheng6Peng Zhang7Haiyang Yu8Jing Zhao9Yanfeng Wang10Mudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaCenter of Edible Fungi, Northwest A&F University, Yangling 712100, ChinaMudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaMudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaMudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaMudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaMudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaMudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaMudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaMudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, ChinaMudanjiang Branch, Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157000, China<i>Sarcomyxa edulis</i> is a characteristic low-temperature, edible mushroom in Northeast China. It has a delicious taste and rich nutritional and medicinal value. <i>S. edulis</i> can undergo explosive fruiting, neat fruiting, and unified harvesting, making it suitable for factory production. The molecular mechanisms underlying fruiting body development in <i>S. edulis</i> remain poorly understood. This study employed transcriptome analysis to compare the post-ripening mycelium (NPM) and primordial fruiting bodies (PRMs) of the thermostable <i>S. edulis</i> strain PQ650759, which uniquely forms primordia under constant temperature. A total of 4862 differentially expressed genes (DEGs) (|log2(fold change)| ≥ 1) were identified and found to be predominantly enriched in biological processes such as cell wall organization, DNA replication, and carbohydrate metabolism. KEGG pathway analysis revealed significant enrichment in 20 metabolic pathways, including mismatch repair, yeast cell cycle, and starch/sucrose metabolism. Ten candidate genes (e.g., SKP1, MRE11, GPI) linked to cell cycle regulation, DNA repair, and energy metabolism were randomly selected and prioritized for functional analysis. Quantitative PCR validation confirmed the reliability of transcriptome data, with expression trends consistent across both methods. Our findings provide critical insights into the molecular regulation of fruiting body development in <i>S. edulis</i> and establish a foundation for future mechanistic studies and strain optimization in industrial cultivation.https://www.mdpi.com/2309-608X/11/7/484<i>Sarcomyxa edulis</i>fruiting body developmenttranscriptome analysis |
| spellingShingle | Zitong Liu Minglei Li Hongyu Ma Fei Wang Lei Shi Jinhe Wang Chunge Sheng Peng Zhang Haiyang Yu Jing Zhao Yanfeng Wang Transcriptomic Profiling of Thermotolerant <i>Sarcomyxa edulis</i> PQ650759 Reveals the Key Genes and Pathways During Fruiting Body Formation Journal of Fungi <i>Sarcomyxa edulis</i> fruiting body development transcriptome analysis |
| title | Transcriptomic Profiling of Thermotolerant <i>Sarcomyxa edulis</i> PQ650759 Reveals the Key Genes and Pathways During Fruiting Body Formation |
| title_full | Transcriptomic Profiling of Thermotolerant <i>Sarcomyxa edulis</i> PQ650759 Reveals the Key Genes and Pathways During Fruiting Body Formation |
| title_fullStr | Transcriptomic Profiling of Thermotolerant <i>Sarcomyxa edulis</i> PQ650759 Reveals the Key Genes and Pathways During Fruiting Body Formation |
| title_full_unstemmed | Transcriptomic Profiling of Thermotolerant <i>Sarcomyxa edulis</i> PQ650759 Reveals the Key Genes and Pathways During Fruiting Body Formation |
| title_short | Transcriptomic Profiling of Thermotolerant <i>Sarcomyxa edulis</i> PQ650759 Reveals the Key Genes and Pathways During Fruiting Body Formation |
| title_sort | transcriptomic profiling of thermotolerant i sarcomyxa edulis i pq650759 reveals the key genes and pathways during fruiting body formation |
| topic | <i>Sarcomyxa edulis</i> fruiting body development transcriptome analysis |
| url | https://www.mdpi.com/2309-608X/11/7/484 |
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