Fecal microbiota transplantation alleviates radiation enteritis by modulating gut microbiota and metabolite profiles
This study investigates the safety and underlying mechanisms of fecal microbiota transplantation (FMT) in treating radiation enteritis (RE). A rat model of RE was established with six groups: NC, RT, H-FMT, modified FMT (M-FMT), L-FMT, and BTAC. The therapeutic effects of FMT were assessed using th...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2025-02-01
|
| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://bjbms.org/ojs/index.php/bjbms/article/view/11835 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823864916721795072 |
|---|---|
| author | Qin Ding Jing Xue Nan Li Zhihui Hu Jianbo Song |
| author_facet | Qin Ding Jing Xue Nan Li Zhihui Hu Jianbo Song |
| author_sort | Qin Ding |
| collection | DOAJ |
| description |
This study investigates the safety and underlying mechanisms of fecal microbiota transplantation (FMT) in treating radiation enteritis (RE). A rat model of RE was established with six groups: NC, RT, H-FMT, modified FMT (M-FMT), L-FMT, and BTAC. The therapeutic effects of FMT were assessed using the Disease Activity Index (DAI), histological analysis, and biochemical tests, including ink-propelling, xylitol exclusion, and enzyme-linked immunosorbent assay (ELISA). Gut microbiota alterations and fecal metabolism were analyzed via 16S rDNA sequencing and targeted metabolomics. The results demonstrated that FMT, particularly in the M-FMT group, effectively alleviated RE by reducing DAI scores, histological damage, and inflammatory markers while enhancing enzyme activity, superoxide dismutase (SOD) levels, and intestinal absorption. FMT also modulated gut microbiota composition, increasing beneficial species, such as Blautia wexlerae and Romboutsia timonensis while decreasing Enterococcus ratti. Metabolomics analysis revealed that FMT influenced niacin, nicotinamide, and starch metabolism, with notable changes in pantothenic acid and fatty acid levels. Spearman correlation analysis further indicated that these microbial shifts were associated with improved metabolic profiles. Overall, FMT mitigates RE by regulating gut microbiota and metabolites, with pantothenic acid and fatty acids emerging as potential therapeutic targets. Further research is needed to explore the underlying mechanisms in greater detail.
|
| format | Article |
| id | doaj-art-b635b184d161416996bb41669c47e94c |
| institution | Kabale University |
| issn | 2831-0896 2831-090X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj-art-b635b184d161416996bb41669c47e94c2025-02-08T16:42:39ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2025-02-0110.17305/bb.2025.11835Fecal microbiota transplantation alleviates radiation enteritis by modulating gut microbiota and metabolite profilesQin Ding0Jing Xue1https://orcid.org/0009-0001-3726-4048Nan Li2Zhihui Hu3Jianbo Song4Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, ChinaThird Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan, ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, ChinaThird Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan, China; Shanxi Provincial Key Laboratory for Translational Nuclear Medicine and Precision Protection, Taiyuan, China This study investigates the safety and underlying mechanisms of fecal microbiota transplantation (FMT) in treating radiation enteritis (RE). A rat model of RE was established with six groups: NC, RT, H-FMT, modified FMT (M-FMT), L-FMT, and BTAC. The therapeutic effects of FMT were assessed using the Disease Activity Index (DAI), histological analysis, and biochemical tests, including ink-propelling, xylitol exclusion, and enzyme-linked immunosorbent assay (ELISA). Gut microbiota alterations and fecal metabolism were analyzed via 16S rDNA sequencing and targeted metabolomics. The results demonstrated that FMT, particularly in the M-FMT group, effectively alleviated RE by reducing DAI scores, histological damage, and inflammatory markers while enhancing enzyme activity, superoxide dismutase (SOD) levels, and intestinal absorption. FMT also modulated gut microbiota composition, increasing beneficial species, such as Blautia wexlerae and Romboutsia timonensis while decreasing Enterococcus ratti. Metabolomics analysis revealed that FMT influenced niacin, nicotinamide, and starch metabolism, with notable changes in pantothenic acid and fatty acid levels. Spearman correlation analysis further indicated that these microbial shifts were associated with improved metabolic profiles. Overall, FMT mitigates RE by regulating gut microbiota and metabolites, with pantothenic acid and fatty acids emerging as potential therapeutic targets. Further research is needed to explore the underlying mechanisms in greater detail. https://bjbms.org/ojs/index.php/bjbms/article/view/11835Fecal microbiota transplantationFMTradiation enteritisREgut microbiotametabolite profiles |
| spellingShingle | Qin Ding Jing Xue Nan Li Zhihui Hu Jianbo Song Fecal microbiota transplantation alleviates radiation enteritis by modulating gut microbiota and metabolite profiles Biomolecules & Biomedicine Fecal microbiota transplantation FMT radiation enteritis RE gut microbiota metabolite profiles |
| title | Fecal microbiota transplantation alleviates radiation enteritis by modulating gut microbiota and metabolite profiles |
| title_full | Fecal microbiota transplantation alleviates radiation enteritis by modulating gut microbiota and metabolite profiles |
| title_fullStr | Fecal microbiota transplantation alleviates radiation enteritis by modulating gut microbiota and metabolite profiles |
| title_full_unstemmed | Fecal microbiota transplantation alleviates radiation enteritis by modulating gut microbiota and metabolite profiles |
| title_short | Fecal microbiota transplantation alleviates radiation enteritis by modulating gut microbiota and metabolite profiles |
| title_sort | fecal microbiota transplantation alleviates radiation enteritis by modulating gut microbiota and metabolite profiles |
| topic | Fecal microbiota transplantation FMT radiation enteritis RE gut microbiota metabolite profiles |
| url | https://bjbms.org/ojs/index.php/bjbms/article/view/11835 |
| work_keys_str_mv | AT qinding fecalmicrobiotatransplantationalleviatesradiationenteritisbymodulatinggutmicrobiotaandmetaboliteprofiles AT jingxue fecalmicrobiotatransplantationalleviatesradiationenteritisbymodulatinggutmicrobiotaandmetaboliteprofiles AT nanli fecalmicrobiotatransplantationalleviatesradiationenteritisbymodulatinggutmicrobiotaandmetaboliteprofiles AT zhihuihu fecalmicrobiotatransplantationalleviatesradiationenteritisbymodulatinggutmicrobiotaandmetaboliteprofiles AT jianbosong fecalmicrobiotatransplantationalleviatesradiationenteritisbymodulatinggutmicrobiotaandmetaboliteprofiles |