Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old rats
Background: Free radicals in excessive concentrations damages cells and accelerate the aging process. Antioxidants found in Centella asiatica (CA) and Acalypha indica (AI) have the potential to prevent oxidative cellular damage. Objective: This study aimed to determine the effect of CA and AI on...
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| Format: | Article |
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Indonesian Society for Biochemistry and Molecular Biology
2022-06-01
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| Series: | Acta Biochimica Indonesiana |
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| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/79 |
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| author | Ninik Mudjihartini Reni Paramita Astrid Mariam Khairani Siregar Estiana Filzadiyanti Pungguri Ayu Nega Sarsanti Erni Purwaningsih |
| author_facet | Ninik Mudjihartini Reni Paramita Astrid Mariam Khairani Siregar Estiana Filzadiyanti Pungguri Ayu Nega Sarsanti Erni Purwaningsih |
| author_sort | Ninik Mudjihartini |
| collection | DOAJ |
| description |
Background: Free radicals in excessive concentrations damages cells and accelerate the aging process. Antioxidants found in Centella asiatica (CA) and Acalypha indica (AI) have the potential to prevent oxidative cellular damage.
Objective: This study aimed to determine the effect of CA and AI on carbonyl and glutathione levels in the brain of older rats.
Methods: 18-month age rats were treated using either AI, CA, or vitamin E. In addition, 18-month age and 2-month age untreated rats were used as a negative control. The brain carbonyl and glutathione levels were measured by Agustyanak and the Elmann method, respectively.
Results: Treatment with CA significantly decreased brain carbonyl levels (2.87 nmol/mL) than the control rats (4.54 nmol/mL). Furthermore, treating AI did not reduce the brain carbonyl and GSH levels in aged brain rats.
Conclusion: Centella asiatica can reduce the protein destruction that occurs with increasing age.
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| format | Article |
| id | doaj-art-b62be05f31d64b00902a5353553c5083 |
| institution | Kabale University |
| issn | 2654-6108 2654-3222 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj-art-b62be05f31d64b00902a5353553c50832025-02-08T03:06:03ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222022-06-015110.32889/actabioina.79Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old ratsNinik Mudjihartini0Reni Paramita1Astrid Mariam Khairani Siregar2Estiana Filzadiyanti3Pungguri Ayu Nega Sarsanti4Erni Purwaningsih5Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia. Jakarta. 10440. IndonesiaDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia. Jakarta. 10440. IndonesiaUndergraduate Programme, Faculty of Medicine, Universitas Indonesia. Jakarta. 10440. IndonesiaUndergraduate Programme, Faculty of Medicine, Universitas Indonesia. Jakarta. 10440. IndonesiaNeuroscience and Brain Development Cluster, Indonesian Medical Education and Research Institute. Jakarta. 10440. IndonesiaDepartement of Pharmacy, Faculty of Medicine, Universitas Indonesia. Jakarta. 10440. Indonesia Background: Free radicals in excessive concentrations damages cells and accelerate the aging process. Antioxidants found in Centella asiatica (CA) and Acalypha indica (AI) have the potential to prevent oxidative cellular damage. Objective: This study aimed to determine the effect of CA and AI on carbonyl and glutathione levels in the brain of older rats. Methods: 18-month age rats were treated using either AI, CA, or vitamin E. In addition, 18-month age and 2-month age untreated rats were used as a negative control. The brain carbonyl and glutathione levels were measured by Agustyanak and the Elmann method, respectively. Results: Treatment with CA significantly decreased brain carbonyl levels (2.87 nmol/mL) than the control rats (4.54 nmol/mL). Furthermore, treating AI did not reduce the brain carbonyl and GSH levels in aged brain rats. Conclusion: Centella asiatica can reduce the protein destruction that occurs with increasing age. https://pbbmi.org/newjurnal/index.php/actabioina/article/view/79Acalypha indicaCentella asiaticacarbonylGSHold rat brain |
| spellingShingle | Ninik Mudjihartini Reni Paramita Astrid Mariam Khairani Siregar Estiana Filzadiyanti Pungguri Ayu Nega Sarsanti Erni Purwaningsih Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old rats Acta Biochimica Indonesiana Acalypha indica Centella asiatica carbonyl GSH old rat brain |
| title | Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old rats |
| title_full | Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old rats |
| title_fullStr | Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old rats |
| title_full_unstemmed | Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old rats |
| title_short | Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old rats |
| title_sort | comparing the effect of centella asiatica l and acalypha indica l treatment to carbonyl and glutathione level in the brains of old rats |
| topic | Acalypha indica Centella asiatica carbonyl GSH old rat brain |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/79 |
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