Subcutaneous Adipose Tissue from Obese and Lean Adults Does Not Release Hepcidin In Vivo
Hepcidin is the main regulator of systemic iron homeostasis and is primarily produced by the liver but is also expressed, at the mRNA-level, in periphery tissues including the subcutaneous and visceral adipose tissue. Obesity is associated with elevated hepcidin concentrations and iron depletion sug...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2011/634861 |
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| author | Lisa Tussing-Humphreys Keith N. Frayn Steven R. Smith Mark Westerman A. Louise Dennis Elizabeta Nemeth Jessica Thomson Cenk Pusatcioglu |
| author_facet | Lisa Tussing-Humphreys Keith N. Frayn Steven R. Smith Mark Westerman A. Louise Dennis Elizabeta Nemeth Jessica Thomson Cenk Pusatcioglu |
| author_sort | Lisa Tussing-Humphreys |
| collection | DOAJ |
| description | Hepcidin is the main regulator of systemic iron homeostasis and is primarily produced by the liver but is also expressed, at the mRNA-level, in periphery tissues including the subcutaneous and visceral adipose tissue. Obesity is associated with elevated hepcidin concentrations and iron depletion suggesting that the exaggerated fat mass in obesity could contribute significantly to circulating hepcidin levels consequently altering iron homeostasis. The objective of this study was to determine if abdominal subcutaneous adipose tissue (AbScAT) releases hepcidin in vivo and if release is modified by obesity. Arterio-venous differences in concentrations of hepcidin were measured across AbScAT in 9 obese and 9 lean adults. Overall (n=18), mean plasma hepcidin concentrations were significantly higher in arterialized compared to AbScAT venous samples [mean difference (arterialized-AbScAT venous plasma hepcidin) = 4.9±9.6 ng/mL, P=0.04]. Net regional release was not calculated because mean venous plasma hepcidin concentrations were lower than mean arterialized concentrations indicating no net release. Significant correlations between AbScAT venous and arterialized plasma hepcidin concentrations with anthropometric variables were not observed. Findings from this vein drainage study suggest there is no net release of hepcidin from the AbScAT depot and thereby no ability to signal systemically, even in obesity. |
| format | Article |
| id | doaj-art-b6248774e862411db8559a171b5bf751 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-b6248774e862411db8559a171b5bf7512025-02-03T01:28:45ZengWileyThe Scientific World Journal1537-744X2011-01-01112197220610.1100/2011/634861634861Subcutaneous Adipose Tissue from Obese and Lean Adults Does Not Release Hepcidin In VivoLisa Tussing-Humphreys0Keith N. Frayn1Steven R. Smith2Mark Westerman3A. Louise Dennis4Elizabeta Nemeth5Jessica Thomson6Cenk Pusatcioglu7USDA Agricultural Research Service, Louisiana State University AgCenter, Knapp Hall, Baton Rouge, LA 70803, USAOxford Center for Diabetes, Endocrinology and Metabolism, University of Oxford, Churchill Hospital, Oxford OX3 7LJ, UKThe Translational Research Institute for Metabolism and Diabetes 2566 Lee Rd, Winter Park, FL 32789, USAIntrinsic LifeSciences LLC, La Jolla, CA 92037, USAUniversity of Oxford and NIHR Oxford Biomedical Research Centre, Oxford OX3 7LJ, UKDivision of Pulmonary and Critical Care Medicine, David Geffen School of Medicine, University of California at Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095, USAUSDA Agricultural Research Service, Louisiana State University AgCenter, Knapp Hall, Baton Rouge, LA 70803, USADepartment of Kinesiology and Nutrition, University of Illinois at Chicago, 1919 W. Taylor Street, Room 650, Chicago, IL 60612, USAHepcidin is the main regulator of systemic iron homeostasis and is primarily produced by the liver but is also expressed, at the mRNA-level, in periphery tissues including the subcutaneous and visceral adipose tissue. Obesity is associated with elevated hepcidin concentrations and iron depletion suggesting that the exaggerated fat mass in obesity could contribute significantly to circulating hepcidin levels consequently altering iron homeostasis. The objective of this study was to determine if abdominal subcutaneous adipose tissue (AbScAT) releases hepcidin in vivo and if release is modified by obesity. Arterio-venous differences in concentrations of hepcidin were measured across AbScAT in 9 obese and 9 lean adults. Overall (n=18), mean plasma hepcidin concentrations were significantly higher in arterialized compared to AbScAT venous samples [mean difference (arterialized-AbScAT venous plasma hepcidin) = 4.9±9.6 ng/mL, P=0.04]. Net regional release was not calculated because mean venous plasma hepcidin concentrations were lower than mean arterialized concentrations indicating no net release. Significant correlations between AbScAT venous and arterialized plasma hepcidin concentrations with anthropometric variables were not observed. Findings from this vein drainage study suggest there is no net release of hepcidin from the AbScAT depot and thereby no ability to signal systemically, even in obesity.http://dx.doi.org/10.1100/2011/634861 |
| spellingShingle | Lisa Tussing-Humphreys Keith N. Frayn Steven R. Smith Mark Westerman A. Louise Dennis Elizabeta Nemeth Jessica Thomson Cenk Pusatcioglu Subcutaneous Adipose Tissue from Obese and Lean Adults Does Not Release Hepcidin In Vivo The Scientific World Journal |
| title | Subcutaneous Adipose Tissue from Obese and Lean Adults Does Not Release Hepcidin In Vivo |
| title_full | Subcutaneous Adipose Tissue from Obese and Lean Adults Does Not Release Hepcidin In Vivo |
| title_fullStr | Subcutaneous Adipose Tissue from Obese and Lean Adults Does Not Release Hepcidin In Vivo |
| title_full_unstemmed | Subcutaneous Adipose Tissue from Obese and Lean Adults Does Not Release Hepcidin In Vivo |
| title_short | Subcutaneous Adipose Tissue from Obese and Lean Adults Does Not Release Hepcidin In Vivo |
| title_sort | subcutaneous adipose tissue from obese and lean adults does not release hepcidin in vivo |
| url | http://dx.doi.org/10.1100/2011/634861 |
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