Cytospora paraplurivora sp. nov. isolated from orchards with fruit tree decline syndrome in Ontario, Canada.
A new species of Cytospora was isolated from cankered wood of Prunus spp. during a survey of orchards exhibiting symptoms of fruit tree decline syndrome in southern Ontario, Canada. We found isolates that are morphologically similar to species in the Cytosporaceae family, which is characterized by s...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0279490&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849683021078200320 |
|---|---|
| author | Evgeny Ilyukhin Hai D T Nguyen Alan J Castle Walid Ellouze |
| author_facet | Evgeny Ilyukhin Hai D T Nguyen Alan J Castle Walid Ellouze |
| author_sort | Evgeny Ilyukhin |
| collection | DOAJ |
| description | A new species of Cytospora was isolated from cankered wood of Prunus spp. during a survey of orchards exhibiting symptoms of fruit tree decline syndrome in southern Ontario, Canada. We found isolates that are morphologically similar to species in the Cytosporaceae family, which is characterized by single or labyrinthine locules, filamentous conidiophores or clavate to elongate obovoid asci and allantoid, hyaline conidia. Multi-gene phylogenetic analysis of ITS, LSU, act and tef1- α showed that the isolates form a distinct clade, sister to Cytospora plurivora. Morphologically, our isolates showed differences in the length of conidia and culture characteristics compared to C. plurivora, suggesting the establishment of a new species. The species is described as Cytospora paraplurivora sp. nov. and placed in the family Cytosporaceae of Diaporthales. Additionally, we sequenced, assembled and characterized the genome of the representative isolate for this new species. The phylogenomic analysis confirms the species order and family level classification. C. paraplurivora sp. nov. has the potential to severely affect stone fruits production, causing cankers and dieback in stressed trees, and eventually leads to tree decline. Pathogenicity tests show that the species is pathogenic to Prunus persica var. persica. |
| format | Article |
| id | doaj-art-b622ff6fec95461ea0df075f72985bd9 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b622ff6fec95461ea0df075f72985bd92025-08-20T03:24:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01181e027949010.1371/journal.pone.0279490Cytospora paraplurivora sp. nov. isolated from orchards with fruit tree decline syndrome in Ontario, Canada.Evgeny IlyukhinHai D T NguyenAlan J CastleWalid EllouzeA new species of Cytospora was isolated from cankered wood of Prunus spp. during a survey of orchards exhibiting symptoms of fruit tree decline syndrome in southern Ontario, Canada. We found isolates that are morphologically similar to species in the Cytosporaceae family, which is characterized by single or labyrinthine locules, filamentous conidiophores or clavate to elongate obovoid asci and allantoid, hyaline conidia. Multi-gene phylogenetic analysis of ITS, LSU, act and tef1- α showed that the isolates form a distinct clade, sister to Cytospora plurivora. Morphologically, our isolates showed differences in the length of conidia and culture characteristics compared to C. plurivora, suggesting the establishment of a new species. The species is described as Cytospora paraplurivora sp. nov. and placed in the family Cytosporaceae of Diaporthales. Additionally, we sequenced, assembled and characterized the genome of the representative isolate for this new species. The phylogenomic analysis confirms the species order and family level classification. C. paraplurivora sp. nov. has the potential to severely affect stone fruits production, causing cankers and dieback in stressed trees, and eventually leads to tree decline. Pathogenicity tests show that the species is pathogenic to Prunus persica var. persica.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0279490&type=printable |
| spellingShingle | Evgeny Ilyukhin Hai D T Nguyen Alan J Castle Walid Ellouze Cytospora paraplurivora sp. nov. isolated from orchards with fruit tree decline syndrome in Ontario, Canada. PLoS ONE |
| title | Cytospora paraplurivora sp. nov. isolated from orchards with fruit tree decline syndrome in Ontario, Canada. |
| title_full | Cytospora paraplurivora sp. nov. isolated from orchards with fruit tree decline syndrome in Ontario, Canada. |
| title_fullStr | Cytospora paraplurivora sp. nov. isolated from orchards with fruit tree decline syndrome in Ontario, Canada. |
| title_full_unstemmed | Cytospora paraplurivora sp. nov. isolated from orchards with fruit tree decline syndrome in Ontario, Canada. |
| title_short | Cytospora paraplurivora sp. nov. isolated from orchards with fruit tree decline syndrome in Ontario, Canada. |
| title_sort | cytospora paraplurivora sp nov isolated from orchards with fruit tree decline syndrome in ontario canada |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0279490&type=printable |
| work_keys_str_mv | AT evgenyilyukhin cytosporaparaplurivoraspnovisolatedfromorchardswithfruittreedeclinesyndromeinontariocanada AT haidtnguyen cytosporaparaplurivoraspnovisolatedfromorchardswithfruittreedeclinesyndromeinontariocanada AT alanjcastle cytosporaparaplurivoraspnovisolatedfromorchardswithfruittreedeclinesyndromeinontariocanada AT walidellouze cytosporaparaplurivoraspnovisolatedfromorchardswithfruittreedeclinesyndromeinontariocanada |