Development of an Integrated Computational Pipeline for PARP‐1 Inhibitor Screening Using Hybrid Virtual Screening and Molecular Dynamics Simulations

Abstract Despite the promising anticancer properties of PARP‐1 inhibitors, their clinical use is hindered by side effects. It is crucial to explore new structural variants of these inhibitors to increase efficacy and minimize side effects, enhancing their clinical viability and therapeutic scope. In...

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Main Authors: Guan Wang, Jingjing Guo, Feng Xu, Mingjuan Ji
Format: Article
Language:English
Published: Wiley-VCH 2025-08-01
Series:ChemistryOpen
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Online Access:https://doi.org/10.1002/open.202500021
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author Guan Wang
Jingjing Guo
Feng Xu
Mingjuan Ji
author_facet Guan Wang
Jingjing Guo
Feng Xu
Mingjuan Ji
author_sort Guan Wang
collection DOAJ
description Abstract Despite the promising anticancer properties of PARP‐1 inhibitors, their clinical use is hindered by side effects. It is crucial to explore new structural variants of these inhibitors to increase efficacy and minimize side effects, enhancing their clinical viability and therapeutic scope. In this study, we developed a virtual screening workflow that synergistically integrates the capabilities of TransFoxMol, KarmaDock, and AutoDock Vina. This workflow not only streamlines the identification of potential inhibitors but also ensures a systematic approach to prioritizing candidates. Through structural clustering, we identified ten promising PARP‐1 inhibitors. Additionally, molecular dynamics simulations and MM/PBSA were employed to elucidate the binding modes of compounds 1, 3, 6, and 9 with PARP‐1, providing valuable insights into their interaction mechanisms and supporting future drug development efforts. This workflow serves as a versatile tool for early‐stage drug discovery, offering a strategic foundation for the rational design of new PARP‐1 inhibitors.
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institution Kabale University
issn 2191-1363
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spelling doaj-art-b62246f20f964df39736b7cb802a3fc22025-08-21T12:05:32ZengWiley-VCHChemistryOpen2191-13632025-08-01148n/an/a10.1002/open.202500021Development of an Integrated Computational Pipeline for PARP‐1 Inhibitor Screening Using Hybrid Virtual Screening and Molecular Dynamics SimulationsGuan Wang0Jingjing Guo1Feng Xu2Mingjuan Ji3Taizhou Vocational and Technical College School of Medicine and Pharmaceutical Engineering Chemical Pharmaceutical Research Institute Taizhou ChinaTaizhou Vocational and Technical College School of Medicine and Pharmaceutical Engineering Chemical Pharmaceutical Research Institute Taizhou ChinaTaizhou Vocational and Technical College School of Medicine and Pharmaceutical Engineering Chemical Pharmaceutical Research Institute Taizhou ChinaZhejiang University of Technology College of pharmacy Hangzhou ChinaAbstract Despite the promising anticancer properties of PARP‐1 inhibitors, their clinical use is hindered by side effects. It is crucial to explore new structural variants of these inhibitors to increase efficacy and minimize side effects, enhancing their clinical viability and therapeutic scope. In this study, we developed a virtual screening workflow that synergistically integrates the capabilities of TransFoxMol, KarmaDock, and AutoDock Vina. This workflow not only streamlines the identification of potential inhibitors but also ensures a systematic approach to prioritizing candidates. Through structural clustering, we identified ten promising PARP‐1 inhibitors. Additionally, molecular dynamics simulations and MM/PBSA were employed to elucidate the binding modes of compounds 1, 3, 6, and 9 with PARP‐1, providing valuable insights into their interaction mechanisms and supporting future drug development efforts. This workflow serves as a versatile tool for early‐stage drug discovery, offering a strategic foundation for the rational design of new PARP‐1 inhibitors.https://doi.org/10.1002/open.202500021PARP-1Hybrid Virtual ScreeningMolecular Dynamics
spellingShingle Guan Wang
Jingjing Guo
Feng Xu
Mingjuan Ji
Development of an Integrated Computational Pipeline for PARP‐1 Inhibitor Screening Using Hybrid Virtual Screening and Molecular Dynamics Simulations
ChemistryOpen
PARP-1
Hybrid Virtual Screening
Molecular Dynamics
title Development of an Integrated Computational Pipeline for PARP‐1 Inhibitor Screening Using Hybrid Virtual Screening and Molecular Dynamics Simulations
title_full Development of an Integrated Computational Pipeline for PARP‐1 Inhibitor Screening Using Hybrid Virtual Screening and Molecular Dynamics Simulations
title_fullStr Development of an Integrated Computational Pipeline for PARP‐1 Inhibitor Screening Using Hybrid Virtual Screening and Molecular Dynamics Simulations
title_full_unstemmed Development of an Integrated Computational Pipeline for PARP‐1 Inhibitor Screening Using Hybrid Virtual Screening and Molecular Dynamics Simulations
title_short Development of an Integrated Computational Pipeline for PARP‐1 Inhibitor Screening Using Hybrid Virtual Screening and Molecular Dynamics Simulations
title_sort development of an integrated computational pipeline for parp 1 inhibitor screening using hybrid virtual screening and molecular dynamics simulations
topic PARP-1
Hybrid Virtual Screening
Molecular Dynamics
url https://doi.org/10.1002/open.202500021
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AT fengxu developmentofanintegratedcomputationalpipelineforparp1inhibitorscreeningusinghybridvirtualscreeningandmoleculardynamicssimulations
AT mingjuanji developmentofanintegratedcomputationalpipelineforparp1inhibitorscreeningusinghybridvirtualscreeningandmoleculardynamicssimulations