Identification of the "Collagen-Macrophage" sub-category of patients with colorectal cancer as an extension of the CMS4 subtype with THBS2 as a therapeutic target
Abstract We identified a subset of patients with colorectal cancer (CRC) enriched with “collagen-TAMs,” designated the CM class, using large integrated colon cancer transcriptome and single-cell transcriptome datasets. This classification system could be used as an extension of the traditional CMS c...
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| Format: | Article |
| Language: | English |
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BMC
2025-05-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-025-03918-8 |
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| author | Shuwen Chen Zhaoyan Jiang Wanxuan Song Chuqiao Lu Yanbing Lin Shiyao Xu Kunxin Xie Li Wan Xiaoqin Yuan |
| author_facet | Shuwen Chen Zhaoyan Jiang Wanxuan Song Chuqiao Lu Yanbing Lin Shiyao Xu Kunxin Xie Li Wan Xiaoqin Yuan |
| author_sort | Shuwen Chen |
| collection | DOAJ |
| description | Abstract We identified a subset of patients with colorectal cancer (CRC) enriched with “collagen-TAMs,” designated the CM class, using large integrated colon cancer transcriptome and single-cell transcriptome datasets. This classification system could be used as an extension of the traditional CMS classification system for CRC to guide more accurate classification and treatment. We also screened CAF-derived THBS2 as a potential biomarker for CM and found that it plays an important role in CRC disease models in vitro and in vivo, promoting tumor development and metastasis as well as TAM recruitment. Targeting THBS2 combined with PD-1 therapy effectively improved the therapeutic effect of immunotherapy in vivo. The CM classification provides a new perspective for CRC treatment, and THBS2, which is highly expressed in CM cases, can be used as a new potential combined target for immunotherapy. |
| format | Article |
| id | doaj-art-b618a15c932d4c0fb616b68fca86851b |
| institution | DOAJ |
| issn | 1471-230X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj-art-b618a15c932d4c0fb616b68fca86851b2025-08-20T03:09:19ZengBMCBMC Gastroenterology1471-230X2025-05-0125111810.1186/s12876-025-03918-8Identification of the "Collagen-Macrophage" sub-category of patients with colorectal cancer as an extension of the CMS4 subtype with THBS2 as a therapeutic targetShuwen Chen0Zhaoyan Jiang1Wanxuan Song2Chuqiao Lu3Yanbing Lin4Shiyao Xu5Kunxin Xie6Li Wan7Xiaoqin Yuan8Department of Anatomy, Histology and Embryology, Nanjing Medical UniversityDepartment of Anatomy, Histology and Embryology, Nanjing Medical UniversityDepartment of Clinical Medicine, First Clinical Medicine College, Nanjing Medical UniversityDepartment of Clinical Medicine, First Clinical Medicine College, Nanjing Medical UniversityResearch Center for Environment and Female Reproductive Health, the Eighth Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Clinical Medicine, First Clinical Medicine College, Nanjing Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical UniversityDepartment of Oncology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityDepartment of Anatomy, Histology and Embryology, Nanjing Medical UniversityAbstract We identified a subset of patients with colorectal cancer (CRC) enriched with “collagen-TAMs,” designated the CM class, using large integrated colon cancer transcriptome and single-cell transcriptome datasets. This classification system could be used as an extension of the traditional CMS classification system for CRC to guide more accurate classification and treatment. We also screened CAF-derived THBS2 as a potential biomarker for CM and found that it plays an important role in CRC disease models in vitro and in vivo, promoting tumor development and metastasis as well as TAM recruitment. Targeting THBS2 combined with PD-1 therapy effectively improved the therapeutic effect of immunotherapy in vivo. The CM classification provides a new perspective for CRC treatment, and THBS2, which is highly expressed in CM cases, can be used as a new potential combined target for immunotherapy.https://doi.org/10.1186/s12876-025-03918-8Colorectal cancerImmunotherapyClassification systemTHBS2 |
| spellingShingle | Shuwen Chen Zhaoyan Jiang Wanxuan Song Chuqiao Lu Yanbing Lin Shiyao Xu Kunxin Xie Li Wan Xiaoqin Yuan Identification of the "Collagen-Macrophage" sub-category of patients with colorectal cancer as an extension of the CMS4 subtype with THBS2 as a therapeutic target BMC Gastroenterology Colorectal cancer Immunotherapy Classification system THBS2 |
| title | Identification of the "Collagen-Macrophage" sub-category of patients with colorectal cancer as an extension of the CMS4 subtype with THBS2 as a therapeutic target |
| title_full | Identification of the "Collagen-Macrophage" sub-category of patients with colorectal cancer as an extension of the CMS4 subtype with THBS2 as a therapeutic target |
| title_fullStr | Identification of the "Collagen-Macrophage" sub-category of patients with colorectal cancer as an extension of the CMS4 subtype with THBS2 as a therapeutic target |
| title_full_unstemmed | Identification of the "Collagen-Macrophage" sub-category of patients with colorectal cancer as an extension of the CMS4 subtype with THBS2 as a therapeutic target |
| title_short | Identification of the "Collagen-Macrophage" sub-category of patients with colorectal cancer as an extension of the CMS4 subtype with THBS2 as a therapeutic target |
| title_sort | identification of the collagen macrophage sub category of patients with colorectal cancer as an extension of the cms4 subtype with thbs2 as a therapeutic target |
| topic | Colorectal cancer Immunotherapy Classification system THBS2 |
| url | https://doi.org/10.1186/s12876-025-03918-8 |
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