Immunological Aspects in Late Phase of Living Donor Liver Transplant Patients: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity
Aim. To evaluate whether the combination of the peripheral blood CD4+ adenosine triphosphate activity (ATP) assay (ImmuKnow assay: IMK assay) and cytochrome P450 3A5 (CYP3A5) genotype assay is useful for monitoring of immunological aspects in the patient followup of more than one year after living d...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/982163 |
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| author | Shugo Mizuno Yuichi Muraki Kaname Nakatani Akihiro Tanemura Naohisa Kuriyama Ichiro Ohsawa Yoshinori Azumi Masashi Kishiwada Masanobu Usui Hiroyuki Sakurai Masami Tabata Norihiko Yamamoto Tomomi Yamada Katsuya Shiraki Yoshiyuki Takei Tsutomu Nobori Masahiro Okuda Shuji Isaji |
| author_facet | Shugo Mizuno Yuichi Muraki Kaname Nakatani Akihiro Tanemura Naohisa Kuriyama Ichiro Ohsawa Yoshinori Azumi Masashi Kishiwada Masanobu Usui Hiroyuki Sakurai Masami Tabata Norihiko Yamamoto Tomomi Yamada Katsuya Shiraki Yoshiyuki Takei Tsutomu Nobori Masahiro Okuda Shuji Isaji |
| author_sort | Shugo Mizuno |
| collection | DOAJ |
| description | Aim. To evaluate whether the combination of the peripheral blood CD4+ adenosine triphosphate activity (ATP) assay (ImmuKnow assay: IMK assay) and cytochrome P450 3A5 (CYP3A5) genotype assay is useful for monitoring of immunological aspects in the patient followup of more than one year after living donor liver transplantation (LDLT). Methods. Forty-nine patients, who underwent LDLT more than one year ago, were randomly screened by using IMK assay from January 2010 to December 2011, and the complete medical records of each patient were obtained. The CYP3A5 genotypes were examined in thirty-nine patients of them. Results. The mean ATP level of the IMK assay was significantly lower in the patients with infection including recurrence of hepatitis C (HCV) () than in those without infection (): 185 versus 350 ng/mL (), while it was significantly higher in the patients with rejection () than in those without rejection (): 663 versus 306 ng/mL (). The IMK assay showed favorable sensitivity/specificity for infection (0.909/0.842) as well as acute rejection (1.0/0.911). CYP3A5 genotypes in both recipient and donor did not affect incidence of infectious complications. Conclusions. In the late phase of LDLT patients, the IMK assay is very useful for monitoring immunological aspects including bacterial infection, recurrence of HCV, and rejection. |
| format | Article |
| id | doaj-art-b611ed07ef3d4001b404b4f6d8978841 |
| institution | OA Journals |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-b611ed07ef3d4001b404b4f6d89788412025-08-20T02:23:19ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/982163982163Immunological Aspects in Late Phase of Living Donor Liver Transplant Patients: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate ActivityShugo Mizuno0Yuichi Muraki1Kaname Nakatani2Akihiro Tanemura3Naohisa Kuriyama4Ichiro Ohsawa5Yoshinori Azumi6Masashi Kishiwada7Masanobu Usui8Hiroyuki Sakurai9Masami Tabata10Norihiko Yamamoto11Tomomi Yamada12Katsuya Shiraki13Yoshiyuki Takei14Tsutomu Nobori15Masahiro Okuda16Shuji Isaji17Department of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Pharmacy, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Molecular and Laboratory Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Gastroenterology and Hepatology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanTranslational Medical Science, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Gastroenterology and Hepatology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Gastroenterology and Hepatology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Molecular and Laboratory Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Pharmacy, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanAim. To evaluate whether the combination of the peripheral blood CD4+ adenosine triphosphate activity (ATP) assay (ImmuKnow assay: IMK assay) and cytochrome P450 3A5 (CYP3A5) genotype assay is useful for monitoring of immunological aspects in the patient followup of more than one year after living donor liver transplantation (LDLT). Methods. Forty-nine patients, who underwent LDLT more than one year ago, were randomly screened by using IMK assay from January 2010 to December 2011, and the complete medical records of each patient were obtained. The CYP3A5 genotypes were examined in thirty-nine patients of them. Results. The mean ATP level of the IMK assay was significantly lower in the patients with infection including recurrence of hepatitis C (HCV) () than in those without infection (): 185 versus 350 ng/mL (), while it was significantly higher in the patients with rejection () than in those without rejection (): 663 versus 306 ng/mL (). The IMK assay showed favorable sensitivity/specificity for infection (0.909/0.842) as well as acute rejection (1.0/0.911). CYP3A5 genotypes in both recipient and donor did not affect incidence of infectious complications. Conclusions. In the late phase of LDLT patients, the IMK assay is very useful for monitoring immunological aspects including bacterial infection, recurrence of HCV, and rejection.http://dx.doi.org/10.1155/2013/982163 |
| spellingShingle | Shugo Mizuno Yuichi Muraki Kaname Nakatani Akihiro Tanemura Naohisa Kuriyama Ichiro Ohsawa Yoshinori Azumi Masashi Kishiwada Masanobu Usui Hiroyuki Sakurai Masami Tabata Norihiko Yamamoto Tomomi Yamada Katsuya Shiraki Yoshiyuki Takei Tsutomu Nobori Masahiro Okuda Shuji Isaji Immunological Aspects in Late Phase of Living Donor Liver Transplant Patients: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity Clinical and Developmental Immunology |
| title | Immunological Aspects in Late Phase of Living Donor Liver Transplant Patients: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity |
| title_full | Immunological Aspects in Late Phase of Living Donor Liver Transplant Patients: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity |
| title_fullStr | Immunological Aspects in Late Phase of Living Donor Liver Transplant Patients: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity |
| title_full_unstemmed | Immunological Aspects in Late Phase of Living Donor Liver Transplant Patients: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity |
| title_short | Immunological Aspects in Late Phase of Living Donor Liver Transplant Patients: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity |
| title_sort | immunological aspects in late phase of living donor liver transplant patients usefulness of monitoring peripheral blood cd4 adenosine triphosphate activity |
| url | http://dx.doi.org/10.1155/2013/982163 |
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