Nanoemulsified Corn Oil in Lactating Barki Nutrition: Effect on Intake, Nutrient Digestibility, Rumen Fermentation Characteristics, and Microbial Population

Nanoemulsified corn oil was tested on twenty-one multiparous lactating Barki ewes (mean ± SD: 3 ± 0.4 parity, 44.3 ± 1.9 kg body weight, 30 ± 2.7 months of age, and 402 ± 23 g/d of prior milk production) randomly allocated to the following treatments (<i>n</i> = 7 ewes/group): Control—a...

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Main Authors: Min Gao, Rong-Qing Li, Mostafa S. A. Khattab, Ahmed M. Abd El Tawab, Yong-Bin Liu, Mohamed El-Sherbiny
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/10/1424
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author Min Gao
Rong-Qing Li
Mostafa S. A. Khattab
Ahmed M. Abd El Tawab
Yong-Bin Liu
Mohamed El-Sherbiny
author_facet Min Gao
Rong-Qing Li
Mostafa S. A. Khattab
Ahmed M. Abd El Tawab
Yong-Bin Liu
Mohamed El-Sherbiny
author_sort Min Gao
collection DOAJ
description Nanoemulsified corn oil was tested on twenty-one multiparous lactating Barki ewes (mean ± SD: 3 ± 0.4 parity, 44.3 ± 1.9 kg body weight, 30 ± 2.7 months of age, and 402 ± 23 g/d of prior milk production) randomly allocated to the following treatments (<i>n</i> = 7 ewes/group): Control—a basal diet consisting of 50% concentrate mixtures and 50% berseem clover; CO—the Control diet + 3% of corn oil; NCO—the Control diet + 3% of nanoemulsified corn oil. A completely randomized design of 25 days of adaptation and 5 days of sampling was employed with seven ewes per treatment. Despite feeding oil according to the recommended values, CO decreased the dry matter intake by 8.3% and 6.7% compared to the Control and NCO, respectively. The negative impact of CO extended to reducing the concentrations of ammonia and total volatile fatty acids in the rumen. On the other hand, NCO had less effect on the biohydrogenation intermediates profile compared to CO; noticeably, higher proportions of unsaturated fatty acid (UFA) were associated with NCO; these results were also supported by an increase in the rumen microbial population with NCO compared to CO, especially the biohydrogenation bacteria, which showed higher abundance with NCO despite the low presence of biohydrogenation intermediates. In conclusion, the NCO demonstrated the ability to decrease the transformation of unsaturated fatty acids into saturated fatty acids in the biohydrogenation environment. This effect was not associated with decreased dry matter intake, changes in nutrient digestibility, or alterations in fermentation patterns.
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spelling doaj-art-b601fd9b43b64420acf863ad21cd8db22025-08-20T01:56:55ZengMDPI AGAnimals2076-26152025-05-011510142410.3390/ani15101424Nanoemulsified Corn Oil in Lactating Barki Nutrition: Effect on Intake, Nutrient Digestibility, Rumen Fermentation Characteristics, and Microbial PopulationMin Gao0Rong-Qing Li1Mostafa S. A. Khattab2Ahmed M. Abd El Tawab3Yong-Bin Liu4Mohamed El-Sherbiny5State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010021, ChinaDepartment of Dairy Science, National Research Centre, 33 Bohouth St., Dokki, Giza 12622, EgyptDepartment of Dairy Science, National Research Centre, 33 Bohouth St., Dokki, Giza 12622, EgyptState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010021, ChinaDepartment of Dairy Science, National Research Centre, 33 Bohouth St., Dokki, Giza 12622, EgyptNanoemulsified corn oil was tested on twenty-one multiparous lactating Barki ewes (mean ± SD: 3 ± 0.4 parity, 44.3 ± 1.9 kg body weight, 30 ± 2.7 months of age, and 402 ± 23 g/d of prior milk production) randomly allocated to the following treatments (<i>n</i> = 7 ewes/group): Control—a basal diet consisting of 50% concentrate mixtures and 50% berseem clover; CO—the Control diet + 3% of corn oil; NCO—the Control diet + 3% of nanoemulsified corn oil. A completely randomized design of 25 days of adaptation and 5 days of sampling was employed with seven ewes per treatment. Despite feeding oil according to the recommended values, CO decreased the dry matter intake by 8.3% and 6.7% compared to the Control and NCO, respectively. The negative impact of CO extended to reducing the concentrations of ammonia and total volatile fatty acids in the rumen. On the other hand, NCO had less effect on the biohydrogenation intermediates profile compared to CO; noticeably, higher proportions of unsaturated fatty acid (UFA) were associated with NCO; these results were also supported by an increase in the rumen microbial population with NCO compared to CO, especially the biohydrogenation bacteria, which showed higher abundance with NCO despite the low presence of biohydrogenation intermediates. In conclusion, the NCO demonstrated the ability to decrease the transformation of unsaturated fatty acids into saturated fatty acids in the biohydrogenation environment. This effect was not associated with decreased dry matter intake, changes in nutrient digestibility, or alterations in fermentation patterns.https://www.mdpi.com/2076-2615/15/10/1424oilnanoemulsionvolatile fatty acidunsaturated fatty acidbiohydrogenationrumen bacteria
spellingShingle Min Gao
Rong-Qing Li
Mostafa S. A. Khattab
Ahmed M. Abd El Tawab
Yong-Bin Liu
Mohamed El-Sherbiny
Nanoemulsified Corn Oil in Lactating Barki Nutrition: Effect on Intake, Nutrient Digestibility, Rumen Fermentation Characteristics, and Microbial Population
Animals
oil
nanoemulsion
volatile fatty acid
unsaturated fatty acid
biohydrogenation
rumen bacteria
title Nanoemulsified Corn Oil in Lactating Barki Nutrition: Effect on Intake, Nutrient Digestibility, Rumen Fermentation Characteristics, and Microbial Population
title_full Nanoemulsified Corn Oil in Lactating Barki Nutrition: Effect on Intake, Nutrient Digestibility, Rumen Fermentation Characteristics, and Microbial Population
title_fullStr Nanoemulsified Corn Oil in Lactating Barki Nutrition: Effect on Intake, Nutrient Digestibility, Rumen Fermentation Characteristics, and Microbial Population
title_full_unstemmed Nanoemulsified Corn Oil in Lactating Barki Nutrition: Effect on Intake, Nutrient Digestibility, Rumen Fermentation Characteristics, and Microbial Population
title_short Nanoemulsified Corn Oil in Lactating Barki Nutrition: Effect on Intake, Nutrient Digestibility, Rumen Fermentation Characteristics, and Microbial Population
title_sort nanoemulsified corn oil in lactating barki nutrition effect on intake nutrient digestibility rumen fermentation characteristics and microbial population
topic oil
nanoemulsion
volatile fatty acid
unsaturated fatty acid
biohydrogenation
rumen bacteria
url https://www.mdpi.com/2076-2615/15/10/1424
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