Effects of Fucoidan and Fucoidan Oligosaccharides in Growth and Quorum Sensing Mediated Virulence Factor of <i>Campylobacter Jejuni</i>
Fucoidan is a sulfated fucan marine polysaccharide with potential therapeutic applications, including antibacterial activity and the control of virulence factors associated with quorum sensing. This study investigates the bioactivity of fucoidan derived from the brown algae <i>Ascophyllum nodo...
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MDPI AG
2025-03-01
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| Series: | Polysaccharides |
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| Online Access: | https://www.mdpi.com/2673-4176/6/2/24 |
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| author | Sharon Palafox Félix Giovanna Sandoval Larios Rosina Cabrera Alfonso García-Galaz José Ángel Huerta-Ocampo Ana María Guzmán-Partida Rosa Idalia Armenta Corral Jose Andrei Sarabia-Sainz Gabriela Ramos Clamont Montfort |
| author_facet | Sharon Palafox Félix Giovanna Sandoval Larios Rosina Cabrera Alfonso García-Galaz José Ángel Huerta-Ocampo Ana María Guzmán-Partida Rosa Idalia Armenta Corral Jose Andrei Sarabia-Sainz Gabriela Ramos Clamont Montfort |
| author_sort | Sharon Palafox Félix |
| collection | DOAJ |
| description | Fucoidan is a sulfated fucan marine polysaccharide with potential therapeutic applications, including antibacterial activity and the control of virulence factors associated with quorum sensing. This study investigates the bioactivity of fucoidan derived from the brown algae <i>Ascophyllum nodosum</i>, as well as their fucoidan oligosaccharides (OFuc; <3 kDa), on the growth, motility, biofilm formation, and adhesion of <i>Campylobacter jejuni</i>, the leading cause of bacterial gastroenteritis worldwide. The results showed that fucoidan decreased the growth rate of <i>C. jejuni</i> at concentrations greater than 25 µg/mL, while no effect was observed with different concentrations (5–100 µg/mL) of OFuc. Neither compound affected bacterial motility. Both fucoidan and OFuc inhibited abiotic biofilm formation and diminished pathogen adhesion in a concentration-dependent manner. The study also found that <i>C. jejuni</i> recognized the fucoidan molecule through an enzyme-like lectin assay (ELLA) showing a lectin-like adhesin-carbohydrate recognition. Overall, these results suggest the potential of fucoidan from <i>A. nodosum</i> for controlling abiotic biofilm formation in the food industry, and they open new avenues for research into the use of fucoidan as a molecule aimed at blocking infections caused by <i>C. jejuni</i>. |
| format | Article |
| id | doaj-art-b5fe87757bdf4c44acb975a60899606f |
| institution | Kabale University |
| issn | 2673-4176 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polysaccharides |
| spelling | doaj-art-b5fe87757bdf4c44acb975a60899606f2025-08-20T03:27:25ZengMDPI AGPolysaccharides2673-41762025-03-01622410.3390/polysaccharides6020024Effects of Fucoidan and Fucoidan Oligosaccharides in Growth and Quorum Sensing Mediated Virulence Factor of <i>Campylobacter Jejuni</i>Sharon Palafox Félix0Giovanna Sandoval Larios1Rosina Cabrera2Alfonso García-Galaz3José Ángel Huerta-Ocampo4Ana María Guzmán-Partida5Rosa Idalia Armenta Corral6Jose Andrei Sarabia-Sainz7Gabriela Ramos Clamont Montfort8Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán # 46, la Victoria, Hermosillo 83304, MexicoCoordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán # 46, la Victoria, Hermosillo 83304, MexicoSECIHTI-Centro de Investigación en Alimentación y Desarrollo A.C. Subsede Hidalgo, Blvd. Santa Catarina, San Agustín Tlaxcala, Hidalgo 42163, MexicoCoordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán # 46, la Victoria, Hermosillo 83304, MexicoCoordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán # 46, la Victoria, Hermosillo 83304, MexicoCoordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán # 46, la Victoria, Hermosillo 83304, MexicoCoordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán # 46, la Victoria, Hermosillo 83304, MexicoDepartamento de Investigación en Física, Universidad de Sonora, Hermosillo 83190, MexicoCoordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán # 46, la Victoria, Hermosillo 83304, MexicoFucoidan is a sulfated fucan marine polysaccharide with potential therapeutic applications, including antibacterial activity and the control of virulence factors associated with quorum sensing. This study investigates the bioactivity of fucoidan derived from the brown algae <i>Ascophyllum nodosum</i>, as well as their fucoidan oligosaccharides (OFuc; <3 kDa), on the growth, motility, biofilm formation, and adhesion of <i>Campylobacter jejuni</i>, the leading cause of bacterial gastroenteritis worldwide. The results showed that fucoidan decreased the growth rate of <i>C. jejuni</i> at concentrations greater than 25 µg/mL, while no effect was observed with different concentrations (5–100 µg/mL) of OFuc. Neither compound affected bacterial motility. Both fucoidan and OFuc inhibited abiotic biofilm formation and diminished pathogen adhesion in a concentration-dependent manner. The study also found that <i>C. jejuni</i> recognized the fucoidan molecule through an enzyme-like lectin assay (ELLA) showing a lectin-like adhesin-carbohydrate recognition. Overall, these results suggest the potential of fucoidan from <i>A. nodosum</i> for controlling abiotic biofilm formation in the food industry, and they open new avenues for research into the use of fucoidan as a molecule aimed at blocking infections caused by <i>C. jejuni</i>.https://www.mdpi.com/2673-4176/6/2/24<i>Campylobacter jejuni</i>virulencefucose-based compoundsanti-biofilmligand mimicry |
| spellingShingle | Sharon Palafox Félix Giovanna Sandoval Larios Rosina Cabrera Alfonso García-Galaz José Ángel Huerta-Ocampo Ana María Guzmán-Partida Rosa Idalia Armenta Corral Jose Andrei Sarabia-Sainz Gabriela Ramos Clamont Montfort Effects of Fucoidan and Fucoidan Oligosaccharides in Growth and Quorum Sensing Mediated Virulence Factor of <i>Campylobacter Jejuni</i> Polysaccharides <i>Campylobacter jejuni</i> virulence fucose-based compounds anti-biofilm ligand mimicry |
| title | Effects of Fucoidan and Fucoidan Oligosaccharides in Growth and Quorum Sensing Mediated Virulence Factor of <i>Campylobacter Jejuni</i> |
| title_full | Effects of Fucoidan and Fucoidan Oligosaccharides in Growth and Quorum Sensing Mediated Virulence Factor of <i>Campylobacter Jejuni</i> |
| title_fullStr | Effects of Fucoidan and Fucoidan Oligosaccharides in Growth and Quorum Sensing Mediated Virulence Factor of <i>Campylobacter Jejuni</i> |
| title_full_unstemmed | Effects of Fucoidan and Fucoidan Oligosaccharides in Growth and Quorum Sensing Mediated Virulence Factor of <i>Campylobacter Jejuni</i> |
| title_short | Effects of Fucoidan and Fucoidan Oligosaccharides in Growth and Quorum Sensing Mediated Virulence Factor of <i>Campylobacter Jejuni</i> |
| title_sort | effects of fucoidan and fucoidan oligosaccharides in growth and quorum sensing mediated virulence factor of i campylobacter jejuni i |
| topic | <i>Campylobacter jejuni</i> virulence fucose-based compounds anti-biofilm ligand mimicry |
| url | https://www.mdpi.com/2673-4176/6/2/24 |
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