Effect of Wall Shear Stress Gradient on Cells: Distribution of Deformation and Rotation
The effect of wall shear stress gradient on the deformation and rotation of each cell was investigated <em>in vitro</em>. To make a Couette-type of shear flow, the culture medium fluid was sandwiched with a constant gap between parallel walls: a lower stationary culture disk, and an uppe...
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| Format: | Article |
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International Institute of Informatics and Cybernetics
2022-04-01
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| Series: | Journal of Systemics, Cybernetics and Informatics |
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| Online Access: | http://www.iiisci.org/Journal/PDV/sci/pdfs/ZA155DQ22.pdf
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| author | Hiroki Yonezawa Shigehiro Hashimoto Ryuya Ono |
| author_facet | Hiroki Yonezawa Shigehiro Hashimoto Ryuya Ono |
| author_sort | Hiroki Yonezawa |
| collection | DOAJ |
| description | The effect of wall shear stress gradient on the deformation and rotation of each cell was investigated <em>in vitro</em>. To make a Couette-type of shear flow, the culture medium fluid was sandwiched with a constant gap between parallel walls: a lower stationary culture disk, and an upper rotating disk. Mouse fat precursor cells (3T3-L1) were used in this experiment. After cultivation without flow for 24 hours for adhesion of cells on the lower plate, a shear stress of less than 2 Pa was continuously applied to cells for 24 hours in the incubator. The behavior (deformation and major axis angle) of each single cell was tracked using time-lapse images observed by an inverted phase contrast microscope placed in the incubator. For wall shear stresses of less than 1.9 Pa, each cell exhibited active behavior: migration, deformation, and rotation. Whereas, the cells transformed into spheres when wall shear stress was higher than 1.9 Pa. In addition, the cells were observed to tilt against for a wall shear stress gradient approaching 50 Pa/m. |
| format | Article |
| id | doaj-art-b5ebd8ff79f74299bc990551ae78a84a |
| institution | OA Journals |
| issn | 1690-4524 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | International Institute of Informatics and Cybernetics |
| record_format | Article |
| series | Journal of Systemics, Cybernetics and Informatics |
| spelling | doaj-art-b5ebd8ff79f74299bc990551ae78a84a2025-08-20T01:47:51ZengInternational Institute of Informatics and CyberneticsJournal of Systemics, Cybernetics and Informatics1690-45242022-04-012023339Effect of Wall Shear Stress Gradient on Cells: Distribution of Deformation and RotationHiroki YonezawaShigehiro HashimotoRyuya OnoThe effect of wall shear stress gradient on the deformation and rotation of each cell was investigated <em>in vitro</em>. To make a Couette-type of shear flow, the culture medium fluid was sandwiched with a constant gap between parallel walls: a lower stationary culture disk, and an upper rotating disk. Mouse fat precursor cells (3T3-L1) were used in this experiment. After cultivation without flow for 24 hours for adhesion of cells on the lower plate, a shear stress of less than 2 Pa was continuously applied to cells for 24 hours in the incubator. The behavior (deformation and major axis angle) of each single cell was tracked using time-lapse images observed by an inverted phase contrast microscope placed in the incubator. For wall shear stresses of less than 1.9 Pa, each cell exhibited active behavior: migration, deformation, and rotation. Whereas, the cells transformed into spheres when wall shear stress was higher than 1.9 Pa. In addition, the cells were observed to tilt against for a wall shear stress gradient approaching 50 Pa/m.http://www.iiisci.org/Journal/PDV/sci/pdfs/ZA155DQ22.pdf biomedical engineeringdeformation and 3t3-l1 cellsshear stress field |
| spellingShingle | Hiroki Yonezawa Shigehiro Hashimoto Ryuya Ono Effect of Wall Shear Stress Gradient on Cells: Distribution of Deformation and Rotation Journal of Systemics, Cybernetics and Informatics biomedical engineering deformation and 3t3-l1 cells shear stress field |
| title | Effect of Wall Shear Stress Gradient on Cells: Distribution of Deformation and Rotation |
| title_full | Effect of Wall Shear Stress Gradient on Cells: Distribution of Deformation and Rotation |
| title_fullStr | Effect of Wall Shear Stress Gradient on Cells: Distribution of Deformation and Rotation |
| title_full_unstemmed | Effect of Wall Shear Stress Gradient on Cells: Distribution of Deformation and Rotation |
| title_short | Effect of Wall Shear Stress Gradient on Cells: Distribution of Deformation and Rotation |
| title_sort | effect of wall shear stress gradient on cells distribution of deformation and rotation |
| topic | biomedical engineering deformation and 3t3-l1 cells shear stress field |
| url | http://www.iiisci.org/Journal/PDV/sci/pdfs/ZA155DQ22.pdf
|
| work_keys_str_mv | AT hirokiyonezawa effectofwallshearstressgradientoncellsdistributionofdeformationandrotation AT shigehirohashimoto effectofwallshearstressgradientoncellsdistributionofdeformationandrotation AT ryuyaono effectofwallshearstressgradientoncellsdistributionofdeformationandrotation |